Skip to main content
Log in

Chemical composition and antifungal effect of anise (Pimpinella anisum L.) fruit oil at ripening stage

  • Physiology and Metabolism
  • Original Articles
  • Published:
Annals of Microbiology Aims and scope Submit manuscript

Abstract

The composition of the essential oil ofPimpinella anisum L fruit is determined by GC and GC-MS. The volatile oil content obtained by hydrodistillation was 1.91%. Ten compounds representing 98.3% of the oil was identified. The main constituents of he oil obtained from dried fruits were trans-a nethole (93.9%) and estragole (2.4%). The olfactorially valuable constituents that were found with concentration higher than 0.06% were (E)-methyeugenol, α-cuparene, α-himachalene, β-bisabolene, p-anisaldehyde and cis-anethole. Also, the different concentrations of anise oil exerted varying levels of inhibitory effects on the mycelial growth off/ternaria alternata, Aspergillus niger andAspergillus parasiticus used in experimental. The results showed that the most effected fungus from anise oil wasA. parasiticus, which is followed byA. niger andA. alternata. Individual of this plant oil may provide a useful to achive adequate shelf-life of foods.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Aboutabl E.A., Sokar N.M., Megid R.M.A. (1995). Composition and antimicrobial activity ofOtostegia fruticosa Forssk. J. Essent. Oil Res., 7: 299–303.

    CAS  Google Scholar 

  • Adams R. (2001). Essential Oil Components by Quadrupole GC/MS Allured Publishing Corp., Carol Stream, IL, USA.

    Google Scholar 

  • Agrios G.N. (1997). Plant Pathology, 4th edn., Academic Pres, London.

    Google Scholar 

  • Akgül A., Kivanç M., Sert S. (1991). Effect of carvacrol on growth and toxin production byAspergillus flavus andAspergillus parasiticus. Sci. Aliment., 11: 361–370.

    Google Scholar 

  • Ashraf M., Siddiqui M.A., Bhatty M.K. (1980). Studies on the essential oils of the Pakistan species of the family Umbelliferae. Pakistan J. Sci. Ind. Res., 23: 211–212.

    CAS  Google Scholar 

  • Askari F., Sefidkon F., Mirza M. (1998). Quantitative and qualitative study of components in essential oil ofPimpinella anisum L. Journal of Pajouhes-Va-Sazandegi, 38: 70–77.

    Google Scholar 

  • Askari F., Sefidkon F., Mozafarian V. (2005). Essential oil composition ofPimpinella aurea D.C. from Iran. Flavour Fragr. J., 20: 115–117.

    Article  CAS  Google Scholar 

  • Anonymous (2005a). Available in: http//www.braingencyclopedia.com/anise.html.

  • Azzouz M.A, Bullerman L.B. (1982). Comparative antimycotic effects of selected herbs, spices, plant components and commercial antifungal agents. J. Food Protect., 45: 1298–1301.

    Google Scholar 

  • Başer K.H.C., Özek T. (1996). Essential oil ofPimpinella aromatica Bieb. from Turkey. J. Essent. Oil Res., 8: 463–464.

    Google Scholar 

  • Baytop T. (1984). Treatment with Plants in Turkey, Istanbul Univ. Publ. Nu. 3255, Istanbul Turkey (in Turkish).

  • Bown D. (1995). Encyclopedia of Herbs and their Uses. Dorling Kindersley, London.

    Google Scholar 

  • Boyraz N., Özcan M. (2006). Inhibition of phytopathogenic fungi by essential oil, hydrosol, ground material and extract of summer savor (Satureja hortensis L.) growing wild in Turkey. Int. J. Food Microbiol., 107: 238–242.

    Article  CAS  PubMed  Google Scholar 

  • Caccioni D.R.L., Guizzardi M. (1998). Inhibition of germination and growth of fruit and vegetable postharvest pathogenic fungi by essential oil components. J. Essent. Oil Res., 6: 173–179.

    Google Scholar 

  • Chevallier A. (1996). The Encyclopedia of Medicinal Plants. Wolfe Publishing Ltd., London.

    Google Scholar 

  • Deans S.G., Svoboda K.P. (1990). The antimicrobial properties of marjoram (Origanum majorana L.) volatile oil. Flavour Fragr. J., 5: 187–190.

    Article  Google Scholar 

  • Dizdaroĝlu T., Balkan C. (1996). Anise: its production, marketing and constrains in Izmir province. Anadolu, 6: 36–53.

    Google Scholar 

  • Dorman H.J.D., Deans S.G. 2000. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of Applied Microbiology 88: 308–316.

    Article  CAS  PubMed  Google Scholar 

  • Embong M.B., Hadzie D., Molnar S. (1977). Essential oil in Alberta anise oil (Pimpinella anisum L.). Can. J. Plant Sci., 59: 681–688.

    Article  Google Scholar 

  • Erkmen O., Özcan M. (2001). Antimicrobial activites of essential oils from Turkish spices. Eur. Food Res. Technol., 212 (6): 658–660.

    Article  Google Scholar 

  • Farag R.S., Daw Z.Y., Abo-Raya S.H. (1989). Influence of some spice essential oils onAspergillus parasiticus growth and production of aflatoxins in a synthetic medium. J. Food Protect., 54: 74–76.

    CAS  Google Scholar 

  • Fawcet C.H., Spencer D.M. (1970). Plant chemoteraphy with natural products. Ann. Rev. Phytopathol., 403–418.

  • Hedrick U.P. (1972). Sturtevant’s Edible Plants of the World. Dover Publications.

  • Ivanic R., Savin K., Robinson F.V. (1983). Essential oil fromPimpinella serbica fruits. Planta Med., 48 (1): 60–61.

    Article  CAS  PubMed  Google Scholar 

  • Kubo I., Schilcher H., Philipsan J.D., Loew A. (1993). Anethol, a synergist of polygodial and wrburganal againstCandida albicans fungi. Acta Horticulture, 332: 191–197.

    Google Scholar 

  • Lawrence D.M. (1984). Progres in essential oils [Anise oil, Calamus oil, Cardamom oils]. Perfumer and Flavorist, 8: 63–65.

    Google Scholar 

  • Omidbaigi R., Hadjiakhoondi A., Saharkhiz M. (2003). Changes in content and chemical composition ofPimpinella anisum oil at various harvest time. J. Essent. Oil Bearing Plants, 6 (1): 46–50.

    CAS  Google Scholar 

  • Özcan M. (1998). Inhibitory effects of spice extracts on the growth ofAspergillus parasiticus NRRL 2999 strain. Z. Lebensm. Unters. Forsch. A, 207: 253–255.

    Article  Google Scholar 

  • Özcan M. (2003). Effect of essential oils of some plants used as thyme on the growth ofAspergillus parasiticus NRRL 2999 strain. J. Essent. Oil Bearing Plants 6(1): 55–59.

    Google Scholar 

  • Özcan M. (2005). Effect of spice hydrosols on the growth ofAspergillus parasiticus NRRL 2999 strain. J. Med. Food, 8 (2): 275–278.

    Article  PubMed  Google Scholar 

  • Phillips R., Foy N. (1990). Herbs. Pan Books Ltd, London.

    Google Scholar 

  • Püskülcü H., Ikiz F. (1989). Introduction to Statistics. Bilgehan Press, Bornova-Izmir, Turkey.

    Google Scholar 

  • Riotte H. (1978). Companion Planting for Succesful Gardening. Garden Way, Vermont, USA.

    Google Scholar 

  • Rodrigues V.M., Rosa P.T.V., Marques M.O.M., Petenale A.S., Meireles M.A.A. (2003). Supercriticial extraction of essential oil from aniseed (Pimpinella anisum L.) using CO2 solubility, kinetics and composition data. J. Agric. Food Chem., 51: 1518–1523.

    Article  CAS  PubMed  Google Scholar 

  • Singh S.P., Rao G.P., Upadyaya P.P. (1998). Fungitoxicity of essential oils of some aromatic plant against sugar cane pathogens. Sugar Cane, 2: 14–17.

    Google Scholar 

  • Sokoviç M., Tzakou O., Pitarokili D., Couladis M. (2002). Antifungal activities of selected aromatic plants growing wild in Greece. Nahrung-Food, 46 (5): 317–320.

    Article  Google Scholar 

  • Shelef L.A. (1983). Antimicrobial effects of spices. J. Food Sci., 6: 29–44.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Mehmet Musa Özcan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Özcan, M.M., Chalchat, J.C. Chemical composition and antifungal effect of anise (Pimpinella anisum L.) fruit oil at ripening stage. Ann. Microbiol. 56, 353–358 (2006). https://doi.org/10.1007/BF03175031

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03175031

Key words

Navigation