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Dopamine receptors status after unilateral nigral 6-OHDA lesion

Autoradiographic andin situ hybridization study in the rat brain

  • Published:
Molecular and Chemical Neuropathology

Abstract

The physiological effects of dopamine (DA) are mediated by several distinct receptor subtypes. The effects of unilateral nigral 6-hydroxydopamine (6-OHDA) lesions on DA receptors were investigated by receptor autoradiography using the D1 selective ligand [3H]SCH 23390 as well as the D2 ligand [3H]spiroperidol. mRNA distribution was studied byin situ hybridization.

Lesioned rats were sacrificed at different time intervals. Receptor binding studies were performed on tissue sections using selective ligands. [35S]UTP labeled RNA probes were prepared from the different cDNA (D1, D2, D3) and used forin situ hybridization. A specific loss of receptor binding sites and mRNA hybridization was found in the lesioned substantia nigra pars compacta (SNc) at all times examined. Receptor binding studies revealed a different time-dependent increase in both D1 and D2 receptors.In situ hybridization showed that only D2 receptor mRNA increased in the caudate-putamen (CPu) of the lesioned side 15 d after 6-OHDA. No changes were observed in D1 and D3 receptor mRNA during the entire time-course.

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Authors and Affiliations

  1. Department of Neurobiology, Farmitalia Carlo Erba-Erbamont Group, 20014 Nerviano, Milan, Italy

    Maria Gioia Fornaretto, Carla Caccia & Ruggero G. Fariello

  2. Department of Cell Biology, Duke University Medical Center, Durham, NC

    Maria Gioia Fornaretto & Marc G. Caron

Authors
  1. Maria Gioia Fornaretto
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  2. Carla Caccia
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  3. Marc G. Caron
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  4. Ruggero G. Fariello
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Fornaretto, M.G., Caccia, C., Caron, M.G. et al. Dopamine receptors status after unilateral nigral 6-OHDA lesion. Molecular and Chemical Neuropathology 19, 147–162 (1993). https://doi.org/10.1007/BF03160175

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  • DOI: https://doi.org/10.1007/BF03160175

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