Abstract
A cDNA encoding glyceraldehyde-3-phosphate dehydrogenase (GPD) was isolated from the fission yeastSchizosaccharomyces pombe. The nucleotide and the predicted amino acid sequences were determined. A very high degree of homology with different GPD sequences present in the SWISS-PROT data bank was observed in accordance with the evolutionary conservation of this enzyme. Northern analysis and the comparison with other fungal GPD sequences suggest that the cDNA corresponds to an activek expressed gene encoding a functional GPD protein. Finally, codon usage analysis revealed the same codor bias observed tor three highly expressed genes inS. pombe.
Riassunto
Un cDNA che codifica per 1a gliceraldeide-3-rostato deidrogenasi (GPD) è stato isolato dal lievito a tissioneSchizosaccharomyces pombe. Sono state determinate sia 1a sequenza nucleotidica che quella amminoacidica predetta. Un alto grado di omologia è stato riscon-trato con diverse sequenze di GPD presenti nella banca dati SWISS-PROT in accordo con 1a conservativita evolutiva di questo enzima. L’analisi di Northern ed il confronto con altre sequenze di GPD fungine sugge-riscono che il cDNA corrisponde ad un gene espresso attivamente codificante per una GPD funzidnale. In fine, l’analisi dell’uso dei codoni rivela 1a stessa scelta preterenziale osserrata per tre geni altamente espressi inS. pombe.
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Nella seduta dell’11 maggio 1996.
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Orlandi, I., Popolo, L., Cavadini, P. et al. Cloning and characterization of a cDNA encoding glyceral-dehyde-3-phosphate dehydrogenase from the fission yeast Schizosaccharomyces pombe. Rend. Fis. Acc. Lincei 7, 315–322 (1996). https://doi.org/10.1007/BF03002248
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DOI: https://doi.org/10.1007/BF03002248