Abstract
Diabetes is known to involve oxidative stress and changes in lipid metabolism. Many secondary plant metabolites have been shown to possess antioxidant activities, improving the effects of oxidative stress on diabetes. This study evaluated the effects of extracts fromGongronema latifolium leaves on antioxidant enzymes and lipid profile in a rat model of non insulin dependent diabetes mellitus (NIDDM). The results confirmed that the untreated diabetic rats were subjected to oxidative stress as indicated by significantly abnormal activities of their scavenging enzymes (low superoxide dismutase and glutathione peroxide activities), compared to treated diabetic rats, and in the extent of lipid peroxidation (high malondialdehyde levels) present in the hepatocytes. The ethanolic extract ofG. latifolium leaves possessed antioxidant activity as shown by increased superoxide dismutase and glutathione peroxidase activities and decreases in malondialdehyde levels. High levels of triglycerides and total cholesterol, which are typical of the diabetic condition, were also found in our rat models of diabetes. The ethanolic extract also significantly decreased triglyceride levels and normalized total cholesterol concentration.
Similar content being viewed by others
Abbreviations
- CAT:
-
Catalase
- GPx:
-
glutathione peroxidase
- MDA:
-
malondialdehyde
- NIDDM:
-
non insulin dependent diabetes mellitus
- ROS:
-
reactive oxygen species
- SOD:
-
superoxide dismutase
- STZ:
-
streptozotocin
- TG:
-
triglyceride
References
Aebi H 1984 Catalasein vitro;Methods Enzymol. 105 121–126
Akhtar M S and Ali M R 1984 Study of antidiabetic effect of a compound medicinal plant prescription in normal and diabetic rabbits;J. Pakistan. Med. Assoc. 34 239–244
Aydin A, Orhan H, Sayal A, Ozata M, Sahin G and Isimer A 2001 Oxidative stress and nitric oxide related parameters in type II diabetes mellitus: effect of glycemic control;Clin. Biochem. 34 65–70
Betteridge D J 1994 Diabetic dyslipidemia;Am. J. Med. (Suppl. 6A)96 25S-31S
Bierman E L 1992 Atherogenesis in diabetes;Arterioscler. Thromb. 12 647–656
Ceriello A, Quatraro A and Giugliano D 1992 New insights on non-enzymatic glycosylation may lead to therapeutic approaches for the prevention of the diabetic complications;Diabet. Med. 9 297–299
Cho S-Y, Park J-Y, Park E M, Choi M S, Lee M-Y, Jeon S M, Jang M-K, Kim M J and Park Y-B 2002 Alternation of hepatic antioxidant enzyme activities and lipid profile in streptozotocin-induced diabetic rats by supplementation of dandelion water extract;Clin. Chim. Acta 317 109–117
Hartnett E M, Stratton R D, Browne R W, Rosner B A, Lanham R J and Armstrong D 2000 Serum Markers of oxidative stress and severity of diabetic retinopathy;Diabet. Care 23 234–240
Hodgson E K and Fridovich I 1975 The interaction of bovine erythrocyte superoxide dismutase with hydrogen peroxide: inactivation of the enzyme;Biochemistry 14 5294–5299
Izawa S, Inoue Y and Kimura A 1996 Importance of catalase in the adaptive response to hydrogen peroxide: analysis ofacatalasaemic Saccharomyces cerevisiae;Biochem. J. (Pt 1) 320 61–67
Kesavulu M M, Giri R, Kameswara R B and Apparao C 2000 Lipid peroxidation and antioxidant enzyme levels in type 2 diabetic with microvascular complications;Diabet. Metabol. 26 387–392
Levy U, Zaltzber H, Ben-Amotz A, Kanter Y and Aviram M 1999 β-Carotene affects antioxidant status in non-insulin dependent diabetes mellitus;Pathophysiology 6 157–161
Morebise O, Fafunso M A, Makinde J M, Olajide O A and Awe E O 2002 Anti-inflammatory property of the leaves ofGongronema latifolium;Phytother. Res. 16 S75-S77
Okafor J C 1981Woody plants of nutritional importance in traditional systems of the Nigerian humid tropics, Ph D thesis, University of Ibadan, Ibadan
Okafor J C 1987Identification and conservation of plants used in traditional medicines (Lead lecture presented at the International Workshop on Evaluation of Traditional Medicine, University of Nigeria, Nsukka)
Okafor J C 1989Tropical plants in health-care delivery (Guest lecture delivered to Pharmacognosy Society of Nigeria at the University of Nigeria, Nsukka)
Orchard T J 1990 Dyslipoproteinemia and diabetes;Endocrinol. Metab. Clin. North Am. 19 361–379
Paglia D and Valentine W 1967 Studies on the quantitative and qualitative characterization of erythtrocyte glutathione peroxidase;J. Lab. Clin. Med. 70 158–169
Scoppola A, Montecchi F R, Mezinger G and Lala A 2001 Urinary mevalonate excretion rate in type 2 diabetes: role of metabolic control;Atherosclerosis 156 357–361
Searle A J and Willson R L 1980 Glutathione peroxidase: effect of superoxide, hydroxyl and bromine free radicals on enzyme activity;Int. J. Radiant. Biol. 37 213–217
Sozmen E Y, Sozmen B, Delen Y and Onat T 2001 Catalase/superoxide dismutase (SOD) and catalase/paraoxonase (PON) ratios may implicate poor glycemic control;Arch. Med. Res. 32 283–287
Sunderman F W Jr, Marzouk A, Hopfer S M, Zaharia O and Reid M C 1985 Increased lipid peroxidation in tissues of nickel chloride-treated rats;Ann. Clin. Lab. Sci. 15 229–236
Uchimura K, Nagasaka A, Hayashi R, Makino M, Nagata M, Kakizawa H, Kobayashi T, Fujiwara K, Kato T, Iwase K, Shinohara R, Kato K and Itoh M 1999 Changes in superoxide dismutase activities and concentrations and myeloperoxidase activities in leukocytes from patients with diabetes mellitus;J. Diabet. Complications 13 264–270
Ukeda H, Maeda S, Ishii T and Sawamura M 1997 Spectrophotometric assay for superoxide dismutase based on tetrazolium salt 3′-l-(phenylamino)-carbonyl-3,4-tetrazolium-bis (4-methoxy-6-nitro) benzenesulfonic acid hydrate reduction by xanthine-xanthine oxidase;Anal. Biochem. 251 206–209
Wolff S P, Jiang Z Y and Shunt J V 1991 Protein glycation and oxidative stress in diabetes mellitus and aging;Free Rad. Biol. Med. 10 339–352
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ugochukwu, N.H., Babady, N.E., Cobourne, M. et al. The effect ofGongronema latifolium extracts on serum lipid profile and oxidative stress in hepatocytes of diabetic rats. J. Biosci. 28, 1–5 (2003). https://doi.org/10.1007/BF02970124
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02970124