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Intratumoral pyrimidine nucleoside phosphorylase (pynpase) activity predicts a selective effect of adjuvant 5’-deoxy-5-fluorouridine (5’ dfur) on breast cancer

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Abstract

Background

Pyrimidine nucleoside phosphorylase (PyNPase) is the enzyme that converts 5’-deoxy-5-fluorouracil (5’DFUR) to 5-fluorouracil (5FU). Its activity in cancer tissue may correlate with the selective antitumor activity of 5’DFUR in breast cancer.

Methods

Two hundred and sixteen T2 breast cancer patients were treated consecutively with surgery followed by 5’DFUR (600 mg/body/day)+tamoxifen (20 mg/body/day) for 2 years. PyNPase activity in breast cancer tissue, determined by high-performance liquid chromatography, ranged from 4.2-626.0 μg FU/mg protein/hr (mean±SD, 203.5±122.4), and the examined patients were divided into two groups: group A (high PyNPase group), cases with the PyNPase activity equal to or more than the mean value of 203.5 μg FU/mg protein/hr, and group B (low PyNPase group), cases with activity less than the mean value.

Results

Although there was no difference in relapse-free survival (RFS) between groups A and B, among node-positive patients (n=83) those in group A tended to have a longer RFS. When divided into subgroups according to estrogen receptor (ER) status, among node-positive and ER-positive tumors (n = 49), the RFS was significantly better in group A than in group B (p<0.05).

Conclusion

Intratumoral PyNPase activity might be of use as a predictor of the effect of adjuvant 5’DFUR on breast cancer.

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Abbreviations

PyNPase:

Pyrimidine nucleoside phosphorylase

5’DFUR:

5’-Deoxy-5-fluorouracil

5FU:

5-Fluorouracil

PD-ECGF:

Platelet-derived endothelial cell growth factor

ER:

Estrogen receptor

PR:

Progesterone receptor

RFS:

Relapse-free survival

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Authors and Affiliations

  1. Sapporo Social Insurance General Hospital, Japan

    Yoshinobu Hata

  2. Hokkaido University School of Medicine, Japan

    Hiromasa Takahashi & Fumiaki Sasaki

  3. National Sapporo Hospital, Hokkaido Cancer Center, Japan

    Masami Ogita

  4. Kushiro Rosai Hospital, Japan

    Junichi Uchino

  5. Otaru Municipal Hospital, Japan

    Masanori Yoshimoto

  6. Konan Hospital, Japan

    Yoshinobu Akasaka

  7. Sapporo Municipal Hospital, Japan

    Yoshimi Nakanishi

  8. Tenshi Hospital, Japan

    Yasuo Sawada

Authors
  1. Yoshinobu Hata
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  2. Hiromasa Takahashi
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  3. Fumiaki Sasaki
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  4. Masami Ogita
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  5. Junichi Uchino
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  6. Masanori Yoshimoto
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  7. Yoshinobu Akasaka
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  8. Yoshimi Nakanishi
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  9. Yasuo Sawada
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Hata, Y., Takahashi, H., Sasaki, F. et al. Intratumoral pyrimidine nucleoside phosphorylase (pynpase) activity predicts a selective effect of adjuvant 5’-deoxy-5-fluorouridine (5’ dfur) on breast cancer. Breast Cancer 7, 37–41 (2000). https://doi.org/10.1007/BF02967186

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  • DOI: https://doi.org/10.1007/BF02967186

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