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An animal model to evaluate the protective efficacy ofHaemophilus influenzae type b conjugate vaccines

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Abstract

An efficacy test of PRP (polyribosylribitol phosphate)-TT (Tetanus toxoid) conjugate vaccines was carried out using BALB/c mice as an animal model by inoculatingHaemophilus influenzae type b (Hib) with a virulence enhancement factor (VEF). Three administrations of the conjugate vaccines at 2-week intervals elicited a significantly high level of PRP antibodies (P<0.0001). The protective activity of the PRP immunization was challenged with either Hib with iron dextran (Hibi) or with a combination of mucin and hemoglobin (Hibmh) as a VEF. The medium lethal dose (LD50) for Hibmh and Hibi was measured as 10 CFU (Colony Forming Unit) and 2.5×108 CFU respectively. Each immunized animal was challenged with five or ten times the LD50 level of bacteria with a VEF. A significant difference in mortality between the immunized and control mice (P<0.01) was observed with the Hibmh challenge inoculation but not with the Hibi challenge inoculation. These results show that a combination of mucin and hemoglobin was able to ehance the virulence of Hib in BALB/c mice to cause a lethal infection, thus suggesting that BALB/c mice introduced to this method can be an effective model animal for testing the protective efficacy ofH. influenzae conjugate vaccines.

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Authors and Affiliations

  1. Green Cross Vaccine Corporation, 449-903, Yongin, Korea

    Hyun Sung Kim, Tae Hyeon Yoo, Yang Suk Jang, Hun Kim, Jin Yong Park & Jong Su Kim

  2. Department of Biotechnology and Bioengineering, Inha University, 402-751, Incheon, Korea

    Hyun Sung Kim & Byung-Ki Hur

  3. Department of Molecular Science and Technology, Ajou University, 442-749, Suwon, Korea

    Yang Suk Jang, Jin Yong Park & Yeon-Woo Ryu

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  1. Hyun Sung Kim
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  2. Tae Hyeon Yoo
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  3. Yang Suk Jang
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  4. Hun Kim
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  5. Jin Yong Park
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  8. Jong Su Kim
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Correspondence to Hyun Sung Kim.

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Kim, H.S., Yoo, T.H., Jang, Y.S. et al. An animal model to evaluate the protective efficacy ofHaemophilus influenzae type b conjugate vaccines. Biotechnol. Bioprocess Eng. 9, 490–494 (2004). https://doi.org/10.1007/BF02933491

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  • DOI: https://doi.org/10.1007/BF02933491

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