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Empirical evaluation of cellulase on enzymatic hydrolysis of waste office paper

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Abstract

Enzymatic hydrolysis of waste office paper was evaluated using three commercial cellulases, Acremonium cellulase, Meicelase, and Cellulosin T2. Varying the enzyme loading from 1 to 10% (w/w) conversion of waste office paper to reducing sugar was investigated. The conversion increased with the increase in the enzyme loading: in the case of enzyme loading of 10% (w/w), Acremonium cellulase yielded 79% conversion of waste office paper, which was 17% higher compared to Meicelase, 13% higher than that of Cellulosin T2. Empirical model for the conversion (%) of waste office paper to reducing sugar (x) was derived from experimental results as follow,x=kE m t (aE+b) wherek, m, a, and d denote empirical constants.E indicates initial enzyme concentration.

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Authors and Affiliations

  1. Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, 836 Ohya, 422-8529, Shizuoka, Japan

    Enoch Y. Park & Yuko Ikeda

  2. Technical Developments Department, Tsukishima Kikai Co., Ltd., 17-15, Tsukuda 2-Chome, 104-0051, Chuo-ku, Japan

    Naoyuki Okuda

Authors
  1. Enoch Y. Park
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  2. Yuko Ikeda
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  3. Naoyuki Okuda
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Correspondence to Enoch Y. Park.

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Park, E.Y., Ikeda, Y. & Okuda, N. Empirical evaluation of cellulase on enzymatic hydrolysis of waste office paper. Biotechnol. Bioprocess Eng. 7, 268–274 (2002). https://doi.org/10.1007/BF02932835

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  • DOI: https://doi.org/10.1007/BF02932835

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