Skip to main content
SpringerLink
Log in

Identification and characterization ofThermoplasma acidophilum 2-keto-3-deoxy-D-gluconate kinase: A new class of sugar kinases

  • Published:
Biotechnology and Bioprocess Engineering Aims and scope Submit manuscript

Abstract

The thermoacidophilic archaeonThermoplasma acidophilum has long been known to utilized-glucosevia the non-phosphorylated Entner-Doudoroff (nED) pathway. We now report the identification of a gene encoding 2-keto-3-deoxy-d-gluconate (KDG) kinase. The discovery of this gene implies the presence of a glycolysis pathway, other than the nED pathway. It was found that Ta0122 in theT. acidophilum genome corresponded to KDG kinase. This enzyme shares no similarity with known KDG kinases, and belongs to a novel class of sugar kinases. Of the five sugars tested only KDG was utilized as a substrate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Verhees, C. H., S. W. Kengen, J. E. Tuininga, G. J. Schut, M. W. Adams, W. M. De Vos, and J. Van der Oost (2003) The unique features of glycolytic pathways in archaea.Biochem. J. 373: 231–246.

    Article  Google Scholar 

  2. Lamble, H. J., N. I. Heyer, S. D. Bull, D. W. Hough, and M. J. Danson (2003) Metabolic pathway promiscuity in the archaeonSulfolobus solfataricus revealed by studies on glucose dehydrogenase and 2-keto-3-deoxygluconate aldolase.J. Biol. Chem. 278: 34066–34072.

    Article  CAS  Google Scholar 

  3. De Rosa, M., A. Gambacorta, B. Nicolaus, P. Giardina, E. Poerio, and V. Buonocore (1984) Glucose metabolism in the extreme thermoacidophilic archaebacteriumSulfolobus solfataricus.Biochem. J. 224: 407–414.

    Google Scholar 

  4. Budgen, N. and M. J. Danson (1986) Metabolism of glucosevia a modified Entner-Doudoroff pathway in the thermoacidophilic archaebacteriumThemoplasma acidophilum.FEBS Lett. 196: 207–210.

    Article  CAS  Google Scholar 

  5. Smith, L. D., N. Budgen, S. J. Bungard, M. J. Danson, and D. W. Hough (1989) Purification and characterization of glucose dehydrogenase from the thermoacidophilic archaebacteriumThermoplasma acidophilum.Biochem. J. 261: 973–977.

    CAS  Google Scholar 

  6. Giardina, P., M. G. De Biasi, M. De Rosa, A. Gambacorta, and V. Buonocore (1986) Glucose dehydrogenase from the thermoacidophilic archaebacteriumSulfolobus solfataricus.Biochem. J. 239: 517–522.

    CAS  Google Scholar 

  7. Kim S. and S. B. Lee (2005) Identification and characterization ofSulfolobus solfataricus D-gluconate dehydratase: A key enzyme in the nonphosphorylated Entner-Doudoroff pathway.Biochem. J. 387: 271–280.

    Article  CAS  Google Scholar 

  8. Lamble, H. J., C. C. Milburn, G. L. Taylor, D. W. Hough, and M. J. Danson (2004) Gluconate dehydratase from the promiscuous Entner-Doudoroff pathway inSulfolobus solfataricus.FEBS Lett. 576: 133–136.

    Article  CAS  Google Scholar 

  9. Buchanan, C. L., H. Connaris, M. J. Danson, C. D. Reeve, and D. W. Hough (1999) An extremely thermostable aldolase fromSulfolobus solfataricus with specificity for nonphosphorylated substrates.Biochem. J. 343: 563–570.

    Article  CAS  Google Scholar 

  10. Noh, M. (2005)Identification and Characterization of Glycerate Kinase from the Thermoacidophilic Archaeon Thermoplasma acidophilum. Master Thesis, Pohang, University of Science and Technology, Pohang, Korea.

    Google Scholar 

  11. Szymona, M. and M. Doudoroff (1958) Carbohydrate metabolism inRhodopseudomonas sphreoides.J. Gen. Microbiol. 22: 167–183.

    Google Scholar 

  12. Bender, R., J. R. Andreesen, and G. Gottschalk (1971) 2-Keto-3-deoxygluconate, an intermediate in the fermentation of gluconate by clostridia.J. Bacteriol. 107: 570–573.

    CAS  Google Scholar 

  13. She, Q., R. K. Singh, F. Confalonieri, Y. Zivanovic, G. Allard, M. J. Awayez, C. C. Chan-Weiher, I. G. Clausen, B. A. Curtis, A. De Moors, G. Erauso, C. Fletcher, P. M. Gordon, I. Heikamp-de Jong, A. C. Jeffries, C. J. Kozera, N. Medina, X. Peng, H. P. Thi-Ngoc, P. Redder, M. E. Schenk, C. Theriault, N. Tolstrup, R. L. Charlebois, W. F. Doolittle, M. Duguet, T. Gaasterland, R. A. Garrett, M. A. Ragan, C. W. Sensen, and J. Van der Oost (2001) The complete genome of the crenarchaeonSulfolobus solfataricus P2.Proc. Natl. Acad. Sci. USA 98: 7833–7840.

    Article  Google Scholar 

  14. Ahmed, H., T. J. Ettema, B. Tjaden, A. C. Geerling, J. Van Der Oost, and B. Siebers (2005) The semi-phosphorylative Entner-Doudoroff pathway in hyperthermophilic archaea: a re-evaluation.Biochem. J. 390: 529–540.

    Article  CAS  Google Scholar 

  15. Searey, D. G. (1976) Thermoplasma acidophilum: intracellular pH and potassium concentration.Biochim. Biophys. Acta 451: 278–286.

    Google Scholar 

  16. Conway, T. (1992) The Entner-Doudoroff pathway: history, physiology and molecular biology.FEMS Microbiol. Rev. 103: 1–27.

    Article  CAS  Google Scholar 

  17. Cynkin, M. A. and G. Ashwell (1960) Uronic acid metabolism in bacteria. IV. Purification and properties of 2-keto-3-deoxy-D-gluconokinase inEscherichia coli.J. Biol. Chem. 255: 1576–1579.

    Google Scholar 

  18. Ohshima, N., E. Inagaki, K. Yasuike, K. Takio, and T. H. Tahirov (2004) Structure ofThermus thermophilus 2-keto-3-deoxygluconate kinase: evidence for recognition of an open chain substrate.J. Mol. Biol. 340: 477–489.

    Article  CAS  Google Scholar 

  19. Berger, M., H. Chen, W. Reutter, and S. Hinderlich (2002) Structure and function of N-acetylglucosamine kinase. Identification of two active site cysteines.Eur. J. Biochem. 269: 4212–4218.

    Article  CAS  Google Scholar 

  20. Bork, P., C. Sander, and A. Valencia (1992) An ATPase domain common to prokaryotic cell cycle proteins, sugar kinases, actin, and hsp70 heat shock proteins.Proc. Natl. Acad. Sci. USA 89: 7290–7294.

    Article  CAS  Google Scholar 

  21. Rost, B., G. Yachdav, and J. Liu (2003) The PredictProtein Server.Nucleic Acids Res. 32: W321-W326.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemical Engineering and Division of Molecular Life and Sciences, Pohang University of Science and Technology, 790-784, Pohang, Korea

    Jin Hwa Jung & Sun Bok Lee

Authors
  1. Jin Hwa Jung
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sun Bok Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sun Bok Lee.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jung, J.H., Lee, S.B. Identification and characterization ofThermoplasma acidophilum 2-keto-3-deoxy-D-gluconate kinase: A new class of sugar kinases. Biotechnol. Bioprocess Eng. 10, 535–539 (2005). https://doi.org/10.1007/BF02932290

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02932290

Keywords

Search

Navigation