Abstract
Pure pyocyanin was prepared from 120 strains ofPseudomonas aeruginosa isolated from different clinical specimens. The modified method used increased the purification 425-fold. The spectra of pyocyanin of all strains were found to be identical with a single major peak at 363 nm. The only difference between these spectra appeared to be the level value absorbance. Strains recovered from different clinical specimens differ in their ability for pigment production. The strains isolated from urine produced higher amounts of pyocyanin than other isolates.
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Al-Shibib A.S.: P.aeruginosa and pyocin. Microbiological, biochemical and genetical studiesPhD Thesis, Baghdad University 1982.
Al-Shibib A., Hassan F.K., Al-Ani F.Y., Al-Mudhaffar S.: Pyocin typing ofPseudomonas aeruginosa isolates from hospitals of Iraq.Indian J.Comp.Microbiol.Immunol.Infect.Dis. 4, 196–197 (1983).
Al-Shibib A., Al-Mudhaffar S., Al-Ani F., Hassan F.: Purification and characterization of pyocins fromPseudomonas aeruginosa.Folia Microbiol. 30, 25–29 (1985).
Ayoub N.S.: Epidemiological markers forP. aeruginosa in general hospitals.MSc Thesis, Baghdad University 1981.
Curtin J.A., Petersdorf R.G., Bennett I.L.: Pseudomonas bacteremia: review of ninety-one cases.Ann.Intern.Med. 54, 1077–1107 (1961).
Darell J.H., Wahba A.H.: Pyocin typing of hospital strains ofP. aeruginosa.J.Clin.Path. 17, 236–242 (1964).
Dcube B., Shriniwas:Pseudomonas pyocyaneous infections in Varanasi.J.Indian Med.Ass. 45, 532–536 (1965).
Habs I.Z.: Untersuchungen über die O-Antigen vonP. aeruginosa.Z.Hyg.Infect.Kr. 144, 218–228 (1957).
Ito S., Kageyama M., Egami E.: Isolation and characterization of pyocins from several strains ofP.aeruginosa.J.Gen.Appl.Microbiol. 16, 205–214 (1970).
Kageyama M.: Studies of pyocin. 1. Physical and chemical properties.J.Biochem. 55, 49–53 (1964).
Knight M., Hartman Z., Young V.M.: A new method of preparation of pyocyanin and demonstration of an unusual bacterial sensitivity.Analyt.Biochem. 95, 19–23 (1979).
Kuroda K., Kageyama M.: Biochemical properties of a new flexous bacteriocins. Pyocin F1 produced byP. aeruginosa.J.Biochem. 85, 7–19 (1979).
Kuroda K., Kageyama M.: Comparative study on F-type pyocins ofP. aeruginosa.J.Biochem. 89, 1721–1736 (1981).
Lee E.G., Walden C.C.: Biosynthesis of pyocyanin by paraffin in hydrocarbon oxidizing strains ofP. aeruginosa.Appl.Microbiol. 17, 520–523 (1969).
Lindberg R.C., Latta R.L., Brame R., Monticrife J.A.: Definitive bacteriophage typing system forP. aeruginosa.Bact.Proc. 15, 81–95 (1964).
Palumbo S.A.: Role of iron and sulfur in pigment and slime formation byP. aeruginosa.J.Bacteriol. 11, 430–436 (1972).
Sano Y., Kageyama M.: Purification and properties of an S-type pyocin AP41.J.Bacteriol. 146, 733–739 (1981).
Subramanian K.A., Prakash A., Shriniwas, Bhujwala R.A.: Post-operative wound infection.Indian J.Surg. 35, 57–64 (1973).
Von Saltza M.H., Last J.A., Stapleton P.G., Rathnum M.L., Neidleman S.L.: Cyanomycin, its identity with pyocyanine.J.Antibiot. 22, 49–54 (1969).
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Al-Ani, F.Y., Al-Shibib, A.S., Khammas, K.M. et al. Pyocyanin preparation fromPseudomonas aeruginosa isolated from heterogeneous clinical materials. Folia Microbiol 31, 215–219 (1986). https://doi.org/10.1007/BF02928003
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DOI: https://doi.org/10.1007/BF02928003