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Acetobacter cellulose pellicle as a temporary skin substitute

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Abstract

A bacterial strain with morphological and biochemical properties close toAcetobacter xylinum has been cultured in nonagitated, inverted sucrose- and yeast water-based medium for the production of thick, smooth, and floating cellulosic pellicles. The cellulose content (>90%, dry weight, depending on the efficiency of water washing) and theβ-d-homopolyglucan nature of these pellicles were assessed by physical, chemical, and enzymatic methods. The apyrogenic bacterial biomass, a minor component of the dried biofilm (BioFill®), is inactivated by ethylene dioxide. Once applied on exudating or bloody tissues, this biofilm displays several advantages as a biological dressing, and hence, it is valuable as a temporary skin substitute in the treatment of skin wounds, such as burns, ulcers, grafts, and as an adjuvant in dermal abrasions.

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Authors and Affiliations

  1. LQBB-Biomass Chemio/Biotechnology Lab, Department of Biochemistry, UFPR, PO Box 19046 (81.504), Curitiba-PR, Brazil

    J. D. Fontana, A. M. De Souza & I. L. Torriani

  2. LACEN-FSCMR, Curitiba-PR, Brazil

    C. K. Fontana

  3. BioFill Produtos Biotecnologicos S/A, Curitiba-PR, Brazil

    J. C. Moreschi, B. J. Gallotti, S. J. De Souza, G. P. Narcisco, J. A. Bichara & L. F. X. Farah

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  1. J. D. Fontana
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  2. A. M. De Souza
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  3. C. K. Fontana
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  4. I. L. Torriani
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  5. J. C. Moreschi
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  6. B. J. Gallotti
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  7. S. J. De Souza
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  8. G. P. Narcisco
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  9. J. A. Bichara
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  10. L. F. X. Farah
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Fontana, J.D., De Souza, A.M., Fontana, C.K. et al. Acetobacter cellulose pellicle as a temporary skin substitute. Appl Biochem Biotechnol 24, 253–264 (1990). https://doi.org/10.1007/BF02920250

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