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Adsorption of cellulases on steam-pretreated willow

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Abstract

The adsorption of cellulases on steam-pretreated willow has been measured for2, 4, and 8 wt% willow and with varying enzyme concentrations (2–100 wt% based on substrate). The enzyme concentration was measured as soluble protein, filter paper activity (FPA), CMC-ase activity, and activity toward willow. The adsorption data were modeled with a Langmuir isotherm. The maximum adsorption capability depends on the method for measurement of the enzyme concentration. The lowest value, 470 mg enzyme/g willow, was obtained for the soluble protein and the highest value, 650 mg/g, for the FPA. For technical applications, a single isotherm can be used for calculation of the adsorption capability of steam-treated willow.

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Abbreviations

Eads :

adsorbed enzyme (g/L)

eads :

Ea/So(g enzyme adsorbed/kg ODM)

eads,max :

maximum adsorbed enzyme (g enzyme/kg ODM)

Eo :

initial enzyme concentration (g/L)

Esup :

enzyme concentration in supernatant (g/L)

K:

adsorption equilibrium constant (g/L)

teSo :

substrate concentration (kg/L)

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Authors and Affiliations

  1. Chemical Engineering I, University of Lund, PO Box 124, S- 221 00, Lund, Sweden

    Mats Galbe, Robert Eklund & Guido Zacchi

Authors
  1. Mats Galbe
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  2. Robert Eklund
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  3. Guido Zacchi
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Galbe, M., Eklund, R. & Zacchi, G. Adsorption of cellulases on steam-pretreated willow. Appl Biochem Biotechnol 24, 87–101 (1990). https://doi.org/10.1007/BF02920236

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  • DOI: https://doi.org/10.1007/BF02920236

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