Skip to main content
SpringerLink
Log in

Enhancement of the immobilization and discrimination of DNA probe on a biosensor using gold nanoparticles

  • Notes
  • Published:
Chinese Science Bulletin

Abstract

Nanoparticles have been widely utilized in biological research in recent years. 1,6-Hexanedithiol was used as the medium in this work to attach Au nanoparticles on the gold plates. We studied the immobilization and hybridization of DNA probes onto the resulted plates using the quartz crystal microbalance method. Approximately 3–5 times adsorption enhancement of HS-DNA on the plates was obtained under our experimental conditions. Using this DNA sensor, the sensitivity was promoted by approximately three times to 0.35 ώg/mL.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Lyon, L. A., Musick, M. D., Smith, P. C. et al., Surface plasmon resonance of colloidal Au-modified gold film, Sensors and Actuators, Ser. B, 1999, 54: 118.

    Article  Google Scholar 

  2. Rick, R. L., Myszka, D. G., Advances in surface plasmon resonance biosensor analysis, Current Opinion in Biotechnology, 2000, 11:54.

    Article  Google Scholar 

  3. O’Sullivan, C. K., Guilbault, G. G., Commercial quartz crystal microbalances—Theory and applications, Biosensors and Bioelectronics, 1999, 14: 663.

    Article  CAS  Google Scholar 

  4. Caruso, F., Rodda, E., Furlong, D. N. et al., DNA binding and hybridization on gold and derivatized surfaces, Sensors and Actuators, Ser. B, 1997, 41: 189.

    Article  Google Scholar 

  5. Talavera, E. M., Alvarez-Pez, J. M., Ballesteros, L. et al., Fluorescein-labeled DNA probes for homogeneous hybridization assays: Application to DNAE. coli renaturation, Applied Spectroscopy, 1997, 51:401.

    Article  CAS  Google Scholar 

  6. Moser, I., Schalkhammer, T., Pitter, F. et al., Surface technique for an electrochemical DNA biosensor, Biosensors and Bioelectronics, 1997, 12: 729.

    Article  CAS  Google Scholar 

  7. Richcket, J., Brecht, A., Gopel, W. et al., Quartz crystal microbalance for quantitative biosensing and characterizing protein multilayers, Biosensor and Bioelectronics, 1997, 12: 567.

    Article  Google Scholar 

  8. Caruso, F., Furlong, D. N., Niikura, K. et al.,In situ measurement of DNA immobilization and hybridization using a 27 MHz quartz crystal microbalance, Colloids and Surfaces, Ser. B: Biointerfaces, 1998, 10: 199.

    Article  CAS  Google Scholar 

  9. Uttenenthaler, E., Koblinger, C., Drost, S. et al., Quartz crystal biosensor for detection of the African Swine Fever disease, Analytica Chimica Acta, 1998, 362: 91.

    Article  Google Scholar 

  10. Freeman, R. G., Grabar, K. C., Allison, K. J. et al., Self-assembled metal colloidal monolayers: An approach to SERS substrates, Science, 1995, 267: 1629.

    Article  PubMed  CAS  Google Scholar 

  11. Mirkin, C. A., Letsinger, R. L., Mucic, R. C. et al., A DNA-based method for rationally assembling nanoparticles into macroscopic materials, Nature, 1996, 382: 607.

    Article  PubMed  CAS  Google Scholar 

  12. Elghanian, R., Storhoff, J. J., Mucic, R. C. et al., Selective colorimetric detection of polynucleotides based on the distance dependent optical properties of gold nanoparticles, Science, 1997, 277: 1078.

    Article  PubMed  CAS  Google Scholar 

  13. Storhoff, J. J., Robert, E., Robert, C. M. et al., One-pot colorimetric differentiation of polynucleotides with single base imperfection using gold nanoparticle probes, J. Am. Chem. Soc., 1998, 120: 1959.

    Article  CAS  Google Scholar 

  14. Slot, J. W., Geuze, H. J., A new method of preparing gold probes for multiple-labeling cytochemistry, Eup. J Cell Biol., 1985, 38(1): 87.

    CAS  Google Scholar 

  15. Caruso, F., Serizawa, T., Furlong, D. N. et al., Quartz crystal microbalance and surface plasmon resonance study of surfactant adsorption onto gold and chromium oxide surface, Langmuir, 1995, 11: 1546.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Colloid and Interface Science, Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, 100080, Beijing, China

    Zhao Hongqiu, Lin Lin, Tang Ji’an & Jiang Long

  2. Deparrtment of Biological Sciences and Botechnology, Tsing Hua University, 100084, Beijing, China

    Duan Mingxing

Authors
  1. Zhao Hongqiu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lin Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tang Ji’an
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Duan Mingxing
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jiang Long
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jiang Long.

About this article

Cite this article

Hongqiu, Z., Lin, L., Ji’an, T. et al. Enhancement of the immobilization and discrimination of DNA probe on a biosensor using gold nanoparticles. Chin. Sci. Bull. 46, 1074–1077 (2001). https://doi.org/10.1007/BF02900680

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02900680

Keywords

Search

Navigation