Abstract
A cDNA library of ovine pituitary DNA in plasmid pBR322 has been constructed by conventional methods with certain modifications. The library was screened using partial cDNAs for ratα-subunit and LHβ. We have isolated cDNA clones for ovineα-subunit and LHβ. The identification of these clones was confirmed by partial sequencing. The clones bear about 80% sequence homology with the respective rat cDNAs in the sequenced regions and hybridize with the rat clones in 5 X SSC at 55°C. The ovine LHβ clone has an insert of about 650 bp and selects an RNA of about 750 bases in a northern blot. The α-subunit cDNA clone has an insert of about 550 bp; it has two internalPst I sites and thus shows restriction-based differences from ratα-subunit cDNA, which does not have anyPst I site.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- LH:
-
Luteinizing hormone
- FSH:
-
follicle stimulating hormone
- TSH:
-
thyroid stimulating hormone
- CG:
-
chorionic gonadotropin
- hCG:
-
human CG
- oLH:
-
ovine LH
- MLV:
-
murine leukemia virus
- AMV:
-
avian myloblastosis virus
References
Acher, R. (1980)Proc. R. Soc. London,B210, 21.
Birnboim, H. C. and Doly, J. (1979)Nucleic Acid Res.,7, 1513.
Chin, W. W., Kronenberg, H. M., Dee, P. C., Maloof, F. and Habener, J. F. (1981)Proc. Natl. Acad. Sci. USA,78, 5329.
Chin, W. W., Goddine, J. E., Klein, D. R., Chang, A. S., Tan, L. K. and Habener, J. F. (1983)Proc. Natl. Acad. Sci. USA,80, 4649.
Chin, W. W. (1985) inThe Pituitary Gland (ed. H. Imura) (New York: Raven Press) p. 103.
Denhadrt, D. T. (1966)Biochem. Biophys. Res. Commun.,23, 641.
Erwin, C. R., Croyle, M. L, Donelson, J. E. and Maurer, R. A. (1983)Biochemistry,22, 4856.
Fiddes, J. C. and Goodman, H. M. (1979)Nature (London),281, 351.
Gerard, G. (1985)Focus,7, 1.
Godine, J. E., Chin, W. W. and Habener, J. F. (1982)J. Biol. Chem.,257, 8368.
Gubler, U. and Hoffman, B. J. (1983)Gene,25, 263.
Kaetzel, D. M., Browne, J. K., Wondisford, F., Nett, T. M., Thomason, A. R. and Nilson, J. H. (1985)Proc. Natl. Acad. Sci. USA,82, 7280.
Lehrach, H., Diamond, D., Wozney, J. M. and Boedtker, H. (1977)Biochemistry,16, 4743.
Mandel, M. and Hige, A. (1970)J. Mol. Biol.,53, 159.
Maniatis, T., Fritsch, E. F. and Sambrook, J. (1982)Molecular Cloning, A Laboratory Manual (Cold Spring Harbor: Cold Spring Harbor Laboratory Press).
Messing, J., Crea, R. and Seeburg, P. H. (1981)Nucleic Acids Res.,9, 309.
Nilson, J. H., Thompson, A. R., Cserbak, M. T., Moneman, C. L., and Woychik, R. P. (1983)J. Biol. chem.,258, 4679.
Pierce, J. C, Faith, M. R., Giudice, L. C. and Reeve, J. R. (1976) inpolypeptide hormones: molecular and cellular aspects (Ciba Foundation Symposium 41) p. 225.
Pierce, J. G. and Parsons, T. F. (1981)Annu. Rev. Biochem.,50, 465.
Retzel, E. F., Collet, M. S. and Faras, A. J. (1980)Biochemistry,19, 513.
Rigby, P. W. J., Dieckmann, M., Rhodes, C. and Berg, P. (1977)J. Mol. Biol.,113, 237.
Sanger, F., Nicklen, S. and Coulson, A. R. (1977)Proc. Natl. Acad. Sci. USA,74, 5463.
Sharma, N. C, Singh, O., Gaur, A., Rao, D. N., Singh, V., Rao, L. V., Sehgal, S., Das, C. and Talwar, G. P. (1986) inImmunological approaches to contraception and promotion of fertility (ed. G. P. Talwar) (New York: Plenum Press) p. 37.
Southern, E. M. (1975)J. Mol. Biol.,98, 503.
Stewart, M. and Stewart, F. (1977)J. Mol. Biol.,116, 175.
Talmadge, K., Vamvakopoulous, N. C. and Fiddes, J. C. (1984)Nature (London),307, 37.
Talwar, G. P., Singh, O., Singh, V., Rao, D. N., Sharma, N. C, Das, C. and Rao, L. V. (1986)Fert. Steril.,46, 120.
Ullrich, A., Shine, J., Chirgwin, J., Pictet, R., Tischer, E., Rutter, W. J. and Goodman, H. M. (1977)Science,196, 1313.
Vaitukaitis, J. L., Ross, G. T., Braunstein, G. D. and Rayford, P. L. (1976)Rec. Prog. Horm. Res.,32, 289.
Yamamoto, K. R., Alberts, B. M., Benzinger, R., Lawhorne, L. and Treiber, G. (1970)Virology,40, 734.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jain, S.K., Chin, W.W. & Talwar, G.P. Isolation and characterization of cDNA clones for α- and β-subunits of ovine luteinizing hormone. J. Biosci. 12, 349–357 (1987). https://doi.org/10.1007/BF02898583
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02898583