Abstract
The role of endogenous ethylene in auxin-mediated tuber sprout growth inhibition was determined in potato (Solanum tuberosum L. cv. Russet Burbank) minitubers. Treatment of tubers with biologically active auxins resulted in a transient, dose-dependent increase in ethylene production and inhibition of sprout growth. Biologically inactive auxin analogs elicited neither response. Continuous exposure to > 0.001 μL L-1 exogenous ethylene inhibited sprout growth in a dose-dependent manner with complete inhibition occurring at ethylene concentrations ≥1 μL L-1. In further studies with the active auxin α-naphthalene acetic acid (NAA), the role of ethylene in auxin-induced sprout growth inhibition was determined using ethylene biosynthesis and action inhibitors. The ethylene biosynthesis inhibitor aminoethoxyvinylglycine reduced NAA-induced ethylene biosynthesis by over 80% but had no effect on sprout growth inhibition. The non-competitive ethylene action inhibitor silver thiosulfate had no effect on NAA-induced sprout growth inhibition. Similarly, both the competitive ethylene action inhibitor 2,5-norbornadiene and the irreversible action inhibitor 1-methylcyclopropene were ineffective in reducing NAA-mediated sprout growth inhibition. Collectively, these results do not support the proposal that, in the case of potato tuber sprouts, auxininduced growth inhibition is mediated by endogenous ethylene action.
Resumen
El rol del etileno endógeno en la inhibición del crecimiento del brote mediado por auxinas fue determinado en minitubérculos de papa (Solanum tuberosum L. cv. Russet Burbank). El tratamiento de tubérculos con auxinas biológicamente activas resultó en un incremento pasajero en la producción de etileno e inhibición de crecimiento del brote dependiente de la dosis. Análogos de enzimas biológicamente activas no produjeron ninguna respuesta. La contínua exposicion de etileno exógeno a una concentración > de 0.001 μL L-1 inhibió el crecimiento de brotes de mariera dependiente de la dosis, con una inhibición completa con concentraciones de etileno > a 1μL L-1. En estudios adicionales con la auxina activa α-naftaleno ácido acético (NAA), se determinó el rol del etileno en la inhibición de crecimiento del brote inducido por auxinas utilizando inhibidores de biosintesis y de accién del etileno. La aminoetoxivinilglicina, inhibidor de la biosíntesis de etileno, inducida por NAA redujo la biosíntesis de etileno en más del 80%, pero no tuvo efecto sobre la inhibición de crecimiento del brote. La acción no competitiva del tiosulfato de plata, inhibidor del etileno no tuvo efecto en la inhibición de crecimiento del brote inducida por NAA. De la misma mariera, tanto la acción competitiva del 2,5-nor-bordarieno, inhibidor del etileno, como la acción irreversible del inhibidor 1-metilcloropeno no fueron efectivos para reducir la inhibición de crecimiento del brote por mediación del NAA. Colectivamente estos resultados no apoyan la opinion de que la inhibición de crecimiento de los brotes de papa inducida por auxinas sea realizada por mediación del etileno endógeno.
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Abbreviations
- ABA:
-
Abscisic acid
- AVG:
-
aminoethoxyvinylglycine
- BNZ:
-
benazolin
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- 2,6-D:
-
2,6-dichlorophenoxyacetic acid
- DMSO:
-
dimethylsulfoxide
- IAA:
-
indole-3-acetic acid
- MCP:
-
1-methylcyclopropene
- NAA:
-
a-naphthalene acetic acid
- 2-NAA:
-
2-naphthalene acetic acid
- NBD:
-
2,5-norbornadiene
- PAA:
-
phenylacetic acid
- STS:
-
silver thiosulfate
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Suttle, J.C. Auxin-induced sprout growth inhibition: Role of endogenous ethylene. Am. J. Pot Res 80, 303–309 (2003). https://doi.org/10.1007/BF02854314
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DOI: https://doi.org/10.1007/BF02854314