Summary
To investigate the effects of estrogen (E2) on telomerase activity and its mechanism in human breast cancer cells, estrogen receptor positive MCF-7 cells were treated with different concentrations of E2. Telomerase activity was measured by using TRAP-ELISA method, the cell cycle phases analyzed by using flow cytometry, and the expression of Cyclin D1 detected by using immunohistochemistry method. The results showed that telomerase activity levels were increased in MCF-7 cells treated with 10−8 mol/L E2 during the observed period (P<0.05), and E2 increased telomerase activity levels in a dose-dependent manner (10−10-10−8 mol/L); Simultaneously, the cell cycle phases of MCF-7 cells treated with 10−8 mol/L E2 were changed significantly: G0/G1 phase decreased from 60.52% to 50.93%, S phase increased from 29.03% to 30.83%; However, the expression of Cyclin D1 was decreased. It was concluded that estrogen can upregulate telomerase activity of MCF-7 cells, and the effect can be blocked by antiestrogen tamoxifen. Its mechanism may be closely associated with modulation of cell cycle phases.
Similar content being viewed by others
References
Hiyama E, Gollahon L, Kataoka Tet al. Telomerase activity in human breast tumors. J Natl Cancer Inst, 1996, 88:116
Rhyu M S. Telomeres, telomerase, and immortality. J Natl Cancer Inst, 1995, 87:884
Zhu X, Kumar R, Mandal Met al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc Natl Acad Sci USA, 1996, 93:6091
Landerberg G, Nielsen N H, Nilsson Pet al. Telomerase activity is associated with cell cycle deregulation in human breast cancer. Cancer Res, 1997, 57:549
Author information
Authors and Affiliations
Additional information
GAO Jinbo, male, born in 1973, Doctor in Charge
Rights and permissions
About this article
Cite this article
Jinbo, G., Daoda, C., Yuan, T. et al. Effect of estrogen on telomerase activity in human breast cancer cells. Current Medical Science 23, 286–287 (2003). https://doi.org/10.1007/BF02829516
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02829516