Abstract
Seven DNA extraction protocols were used to obtain DNA from herbarium specimens ofJuncus andLuzula (Juncaceae) of various ages. DNA of historical samples is difficult to extract, and the extracts are seldom of good quality. The quality of DNA obtained was estimated by using a spectrophotometer to measure the A260/280 absorbance ratio. The total DNA yield was measured by a fluorometer. The results indicate the success of using both mixer mill grinding and a DNeasy Plant Kit. Another extraction protocol (grinding with mortar and pestle, using liquid nitrogen) yielded DNA from many samples. Modified CTAB extraction, with a lengthy precipitation, usually provided good amounts of DNA. Other protocols did not give satisfactory results.
Abbreviations
- CTAB:
-
hexadecyltrimetylammonium bromide
- EtOH:
-
ethanol
References
Bacci M, Checcuccii A, Checcuccii G, and Palandri MR (1983) Microwave drying of herbarium specimens. Taxon 34: 649–653.
Balsev H (1996) Juncaceae, Flora Neotropica, Monograph 68, pp 167, Organization for Flora Neotropica, The New York Botanical Garden, New York.
Cobb BD and Clarson JM (1994) A simple procedure for optimizing the polymerase chain reaction (PCR) using modified Taguchi methods. Nucleic Acid Res 22/18: 3801–3805.
Csaikl UM, Bastian H, Brettschneider R, Gauch S, Meir A, Schauerte M, Scholz F, Sperisen C, Vornam B, and Ziegenhagen B (1998) Comparative analysis of different DNA extraction protocols: A fast, universal maxi-preparation of high quality plant DNA for genetic evaluation and phylogenetic studies. Plant Mol Biol Rep 16: 69–86.
Doyle JJ and Doyle JL (1987) A rapid DNA isolation procedure for small quatities of fresh leaf tissue. Phytochem Bull 19: 11–15.
Doyle JJ and Doyle JL (1990): Isolation of plant DNA from fresh tissue. Focus 12/1: 13–15.
Hall DW (1981) Microwave: a method to control herbarium insects. Taxon 30: 818–819.
Hiesinger M, Löffert D, Ritt CH, and Oelmüller U (2001) The effects of phenol on nucleic acid preparation and downstream applications. Qiagen News 5: 23–26.
Hill SR (1983) Micowave and the herbarium specimen: potential dangers. Taxon 32: 614–615.
Jansen RK, Loockerman DJ, and Hyi-Gyung Kim WD (1999) DNA Sampling from Herbarium Material: A Current Perspective. In: Metsger DA and Byers SC (eds) Managing the modern herbarium, An interdisciplinary approach. Society for the preservation of natural history collections, Washington, DC, pp 277–286.
Metsger DA and Byers SC (1999) Managing the modern herbarium, an interdisciplinary approach. Society for the preservation of natural history collections, Washington DC, pp 384.
Ristaino JB, Groves CT, and Parra GR (2001) PCR amplification of the Irish potato famine pathogen from historic specimens. Nature 411(6838): 695–697.
Rogers SO and Bendich AJ (1985) Extraction of DNA from milligram amounts of fresh, herbarium, and mummified plant tissue. Plant Mol Biol 5: 69–76.
Rogers SO (1994) Phylogenetic and taxonomic information from herbarium and mumified DNA. In: Adams, RP et al. (eds) Conservation of plant genes II.: Utilization of ancient and modern DNA. Miss Bot Gard, Monogr Vol. 48.
Ŝtorchová H, Hrdlièková R, Chrtek Jr J, Tetera M, Fritze D, and Fehrer J (2000) An improved method of DNA isolation from plants collected in the field and conserved in saturated NaCl/CTAB solution. Taxon 49: 79–84.
Taberlet P, Gielly L, Pautou G, and Bouvet J (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol Biol 17: 1105–1109.
Taylor JW and Swann EC (1994): Dried samples: soft tissues, DNA from herbarium specimens. In: Herrmann, B and Hummel, S. (eds), Ancient DNA- Springer Verlag.
Wittzell H (1999) Chloroplast DNA variation and reticulate evolution in sexual and apomictic sections of dandelions. Mol Ecol 8: 2023–2035.
Ziegenhagen B and Scholz F (1998) Methods for difficult plant species/tissues. In: Karp A, Isaac PG, and Ingram DS, Molecular tools for screening biodiversity., Plants and animals. Chapman and Hall.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Drábková, L., Kirschner, J. & Vlĉek, Ĉ. Comparison of seven DNA extraction and amplification protocols in historical herbarium specimens of juncaceae. Plant Mol Biol Rep 20, 161–175 (2002). https://doi.org/10.1007/BF02799431
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02799431