Abstract
The exopolysaccharide produced byVolcaniella eurihalina, an halophilic eubacterium, under different environmental and nutritional conditions, is studied.V. eurihalina synthesizes an acidic heteropolysaccharide, composed by rhamnose, glucose, and mannose, as well as amino sugars, uronic acids, and acetyl and sulphate residues. This composition varies depending on the nutrients of culture medium. Viscosity and pseudoplasticity of the polymer solutions are also influenced by the nutritional conditions in which the microorganism was grown.
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Sutherland, I. W. (1990),Biotechnology of Microbial Exopolysaccharides, vol. 9, Cambridge University Press, Cambridge.
Bryan, B. B., Linhardt, R. L., and Daniels, L. (1986),Appl. Environ. Microbiol. 51(6), 1304–1308.
Cerning, J., Renard, C. M. G. C., Thibault, J. F., Bouillanne, C., Landon, M., Desmazeaud, M., and Topisirovic, L. (1994),Appl. Environ. Microbiol. 60(11), 3914–3919.
Kojic, M., Vujcic, M., Banina, A., Cocconcelli, P., Cerning, J., and Topisirovic, L. (1992),Appl. Environ. Microbiol. 58(12), 4086–4088.
Lohman, D. (1990), inNovel Biodegradable Microbial Polymers, Dawes, E. A., ed., Kluver Academic, Netherland, pp. 333–348.
Quesada, E., Valderrama, M. J., Bejar, V., Ventosa, A., Gutierrez, M. C., Ruiz-Berraquero, F., and Ramos-Cormenzana, A. (1990),Int. J. Syst. Bacteriol. 40(3), 261–267.
Quesada, E., Bejar, V., and Calvo, C. (1993),Experientia 49(12), 1037–1041.
Calvo, C., Ferrer, M. R., Martinez-Checa, F., Bejar, V., and Quesada, E. (1995),Appl. Biochem. Biotechnol. 55(1), 45–54.
Moraine, R. A. and Rogovin, P. (1966),Biotechn. Bioeng. 8, 511–524.
Rodriguez-Valera, F., Ruiz-Berraquero, F., and Ramos-Cormenzana, A. (1981),Microb. Ecol. 7, 235–243.
Ng, T. K. and Hu, W. S. (1989),Appl. Microbiol. Biotechnol. 31, 480–485.
Quesada, E., Del Moral, A., and Bejar, V. (1994),Biotechnology Techniques 8(10), 701–706.
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F. (1956),Anal. Chem. 28, 350–356.
Lowry, O. H., Rosebrough, N.J., Farr, A. L., and Randall, R. J. (1951),J. Biol. Chem. 193, 265–275.
Johnson, A. R. (1971),Anal. Biochem. 44, 628–635.
Bergmeyer, H. V. and Bernt, E. (1974),Methods of enzymatic analysis, vol 3, Academic, New York, pp. 1205–1215.
Blumenkrantz, N. and Asboe-Hansen, G. (1973),Anal. Biochem. 54, 484–489.
Dodgson, K. S. and Price, R. G. (1962),Biochem. J. 84, 106–110.
McComb, E. A. and Mc Cready, R. M. (1957),Anal. Chem. 29, 819–821.
Sweeley, C. C., Bentley, R., Makita, M., and Wells, W. W. (1963),Gas Chrom. Sug. 85, 2497–2507.
Dasinger, B. L., McArthur, H. A. I., Lengen, J. P., Smogowicz, A. A., Miller, J. W., O’Neill, J. J., Horton, D., and Costa, J. B. (1994),Appl. Environ. Microbiol. 60(4), 1364–1366.
De la Vega, M. G., Cejudo F. J., and Panque, A. (1991),Appl. Biochem. and Biotechnol. 30(3), 273–284.
Novak, J. S., Tanenbaum, S. W., and Nakas, J. P. (1992),Appl. Environ. Microbiol. 58(11), 3501–3507.
Anton, J., Meseguer, I., and Rodriguez-Valera, F. (1988),Appl. Environ. Microbiol. 54(10), 2381–2386.
Kang, K. K., Veeder, G. T., Mirrasoul, P. J., Kaneko, T., and Cottrell, I. W. (1982),Appl. Environ. Microbiol. 43(5), 1086–1091.
Pfiffner, S. M., McInerney, M. J., Jenneman, G. E., and Knapp, R. M. (1986),Appl. Environ. Microbiol. 51(6), 1224–1229.
Inoue, K., Korenaga, H., Tanaka, N. G., Sakamoto, N., and Kadoya, S. (1988),Carbohydr. Res. 81, 135–142.
Matsuda, M. and Worawattanamateekul, W. (1992),Nippon Susian Gakkaishi 58, 1735–1741.
Okutani, K. and Shigeta, S. (1993),Nippon Suisan Gakkaishi 59(8), 1433–1438.
Tait, M. I., Sutherland, I. W., and Clarke-Sturman, A. J. (1986),J. Gen. Microbiol. 132, 1483–1492.
Uhlinger, D. J. and White, D. C. (1983),Appl. Environ. Microbiol. 45(1), 64–70.
Sutherland, I. W. (1992),Food Biotechnol. 6(1), 75–86.
Cesáro, A., Gamini, A., and Navarini, L. (1992),Polymer 33(19), 4001–4008.
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Bejar, V., Calvo, C., Moliz, J. et al. Effect of growth conditions on the rheological properties and chemical composition ofVolcaniella eurihalina exopolysaccharide. Appl Biochem Biotechnol 59, 77–86 (1996). https://doi.org/10.1007/BF02787859
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DOI: https://doi.org/10.1007/BF02787859