Skip to main content
Log in

Characterization of the genome ofOryctes baculovirus, a viral biocide of the insect pestOryctes rhinoceros

  • Published:
Journal of Biosciences Aims and scope Submit manuscript

Abstract

Oryctes baculovirus is a viral biocide exploited for the control of the insect pestOryctes rhinoceros. We have recently established a physical map of the genome of the Indian isolate ofOryctes baculovirus (OBV-KI). Here we examine the genomic relatedness between OBV-KI and OBV-PV505, the type isolate (originally from the Philippines), by DNA reassociation kinetics and by the use of restriction endonucleases. On the basis of differences in restriction-enzyme profiles between the two genomes, and previously reported differences in protein profiles and antigenic makeup, we propose the taxonomic status of a variant ofOryctes baculovirus for the Indian isolate

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Abbreviations

OBV:

Oryctes baculovirus

OBV-KI or KI:

Kerala isolate of OBV

OBV-PV505 or PV5O5:

Philippine isolate of OBV

OBV-MI:

Malaysian isolate

References

  • Bedford G O 1981 Control of the rhinoceros beetle by baculovirus; inMicrobial control of pests and plant diseases (ed) H D Burgess (London: Academic Press) pp 418–426

    Google Scholar 

  • Burand J P and Summers M D 1982 Alteration ofAutographa californica nuclear polyhidrosis virus DNA upon serial passage in cell culture;Virology 119 223–229

    Article  CAS  Google Scholar 

  • Crawford A M and Sheehan C 1985 Replication ofOryctes baculovirus in cell culture: viral morphogenesis, infectivity and protein synthesis;J. Gen. Virol. 66 529–539

    CAS  Google Scholar 

  • Crawford A M and Zelazny 1990 Evolution inOryctes baculovirus: rate and types of genomic change;Virology 174 294–298

    Article  PubMed  CAS  Google Scholar 

  • Crawford A M, Ashbridge K, Sheehan C and Faulkner P 1985 A physical map ofOryctes baculovirus genome;J. Gen. Virol. 66 2649–2658

    CAS  Google Scholar 

  • Crawford A M, Zelazny B and Alfiler A R 1986 Genotypic variation in geographical isolates ofOryctes baculovirus;J. Gen. Virol. 67 949–952

    Google Scholar 

  • Crook N E, Spencer R A, Payne C C and Leisy D J 1985 Variation inCydia pomonella granulosis virus isolates and physical maps of the DNA from three variants;J. Gen. Virol. 66 2423–2430

    CAS  Google Scholar 

  • Fraser M J, Smith G E and Summers M D 1983 Acquisition of host cell DNA sequences by baculoviruses: Relationship between host DNA insertions and FP mutants ofAutographa californica andGalleria mellonella nuclear polyhedrosis viruses;J. Virol. 47 287–300

    PubMed  CAS  Google Scholar 

  • Fraser M J, Brusca J S, Smith G E and Summers M D 1985 Transposon-mediated mutagenesis of a baculovirus;Virology 145 356–361

    Article  PubMed  CAS  Google Scholar 

  • Huger A M 1966 A virus disease of the Indian rhinoceros beetle,Oryctes rhinoceros (Linnaeus), caused by a new type of insect virus,Rhabdionvirus Oryctes gen.n., sp.n.;J. Invertebr. Pathol. 8 38–51.

    Article  PubMed  CAS  Google Scholar 

  • Maniatis T, Fritsch E F and Sambrook J 1982Molecular cloning, A laboratory manual (Cold Spring Harbor: Cold Spring Harbor Laboratory Press) p. 85

    Google Scholar 

  • Matthews R E F 1982 Classification and nomenclature of viruses, Fourth report of the International Committee on Taxonomy of Viruses;Intervirology 17 1–199

    Google Scholar 

  • Mclntosh A H, Rice W C and Ignoffo C M 1987 Genotypic variants in wild-type populations of baculoviruses; inBiotechnology in invertebrate pathology and cell culture (ed.) K Maramorosch (London: Academic Press) pp 305–321

    Google Scholar 

  • Miller D W and Miller L K 1982 A virus mutant with an insertion of a copia-like transposable element;Nature (London) 299 562–564

    Article  CAS  Google Scholar 

  • Miller L K 1984 Exploring the genome organization of baculoviruses; inMethods in virology (eds) K Maramorosch and H Koprowski (New York: Academic Press) voI7, pp 227–258

    Google Scholar 

  • Mohan K S 1990Isolation and characterization of Oryctes baculovirus (Indian isolate, KI) and its use in the biological control of the insect pest Oryctes rhinoceros (L.), Ph.D. thesis, Indian Institute of Science, Bangalore

    Google Scholar 

  • Mohan K S and Gopinathan K P 1989a Characterization of viral proteins ofOryctes baculovirus and comparison between two geographical isolates;Arch. Virol. 109 207–222

    Article  PubMed  CAS  Google Scholar 

  • Mohan K S and Gopinathan K P 1989b Quantitation of serological cross reactivity between two geographical isolates ofOryctes baculovirus by a modified ELISA;J. Virol. Methods 24 203–214

    Article  PubMed  CAS  Google Scholar 

  • Mohan K S and Gopinathan KP 1991 Physical mapping of the genomic DNA of theOryctes rhinoceros baculovirus, KI;Gene 107 343–344

    Article  PubMed  CAS  Google Scholar 

  • Mohan K S, Jayapal S P and Pillai G B 1983 Baculovirus diseases inOryctes rhinoceros population in Kerala;J. Plant Crops 11 154–161

    Google Scholar 

  • Patel C V and Gopinathan K P 1987 Determination of trace amounts of 5-methylcytosine in DNA by reverse phase HPLC;Anal. Biochem. 164 164–169

    Article  PubMed  CAS  Google Scholar 

  • Payne C C 1974 The isolation and characterization of a virus fromOryctes rhinoceros;J. Gen, Virol. 25 105–116

    CAS  Google Scholar 

  • Purrini K 1989 BaculovirusOryctes release intoOryctes monoceros population in Tanzania, with special reference to the interaction of virus isolates used in our laboratory infection experiments;J. Invertebr. Pathol. 53 285–300

    Article  Google Scholar 

  • Rohrmann G F, McParland R H, Martignoni M E and Beaudreau G S 1978 Genetic relatedness of two nuclear polyhedrosis viruses pathogenic forOrgyia pesudotsugata;Virology 84 213–217

    Article  PubMed  CAS  Google Scholar 

  • Rigby P W J, Dieckmann M, Rhodes C and Berg P 1977 Labelling deoxyribonucleic acid to high specific activityin vitro by nick translation with DNA polymerase I;J. Mol. Biol. 113 237–251

    Article  PubMed  CAS  Google Scholar 

  • Smith IRL and Crook N E 1988In vivo isolation of baculovirus genotypes;Virology 166 240–244

    Article  PubMed  CAS  Google Scholar 

  • Wagner G W and Paschke J D 1977 A comparison of the DNAs of the “R” and “T” strains of mosquito iridescent virus;Virology 81 298–308

    Article  PubMed  CAS  Google Scholar 

  • Zelazny B 1977 Occurrence of the baculovirus disease of the coconut palm rhinoceros beetle in the Philippines and Indonesia;FAO Plant Prot. Bull. 25 73–77

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mohan, K.S., Gopinathan, K.P. Characterization of the genome ofOryctes baculovirus, a viral biocide of the insect pestOryctes rhinoceros . J Biosci 17, 421–430 (1992). https://doi.org/10.1007/BF02720097

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02720097

Keywords

Navigation