Abstract
Oryctes baculovirus is a viral biocide exploited for the control of the insect pestOryctes rhinoceros. We have recently established a physical map of the genome of the Indian isolate ofOryctes baculovirus (OBV-KI). Here we examine the genomic relatedness between OBV-KI and OBV-PV505, the type isolate (originally from the Philippines), by DNA reassociation kinetics and by the use of restriction endonucleases. On the basis of differences in restriction-enzyme profiles between the two genomes, and previously reported differences in protein profiles and antigenic makeup, we propose the taxonomic status of a variant ofOryctes baculovirus for the Indian isolate
Similar content being viewed by others
Abbreviations
- OBV:
-
Oryctes baculovirus
- OBV-KI or KI:
-
Kerala isolate of OBV
- OBV-PV505 or PV5O5:
-
Philippine isolate of OBV
- OBV-MI:
-
Malaysian isolate
References
Bedford G O 1981 Control of the rhinoceros beetle by baculovirus; inMicrobial control of pests and plant diseases (ed) H D Burgess (London: Academic Press) pp 418–426
Burand J P and Summers M D 1982 Alteration ofAutographa californica nuclear polyhidrosis virus DNA upon serial passage in cell culture;Virology 119 223–229
Crawford A M and Sheehan C 1985 Replication ofOryctes baculovirus in cell culture: viral morphogenesis, infectivity and protein synthesis;J. Gen. Virol. 66 529–539
Crawford A M and Zelazny 1990 Evolution inOryctes baculovirus: rate and types of genomic change;Virology 174 294–298
Crawford A M, Ashbridge K, Sheehan C and Faulkner P 1985 A physical map ofOryctes baculovirus genome;J. Gen. Virol. 66 2649–2658
Crawford A M, Zelazny B and Alfiler A R 1986 Genotypic variation in geographical isolates ofOryctes baculovirus;J. Gen. Virol. 67 949–952
Crook N E, Spencer R A, Payne C C and Leisy D J 1985 Variation inCydia pomonella granulosis virus isolates and physical maps of the DNA from three variants;J. Gen. Virol. 66 2423–2430
Fraser M J, Smith G E and Summers M D 1983 Acquisition of host cell DNA sequences by baculoviruses: Relationship between host DNA insertions and FP mutants ofAutographa californica andGalleria mellonella nuclear polyhedrosis viruses;J. Virol. 47 287–300
Fraser M J, Brusca J S, Smith G E and Summers M D 1985 Transposon-mediated mutagenesis of a baculovirus;Virology 145 356–361
Huger A M 1966 A virus disease of the Indian rhinoceros beetle,Oryctes rhinoceros (Linnaeus), caused by a new type of insect virus,Rhabdionvirus Oryctes gen.n., sp.n.;J. Invertebr. Pathol. 8 38–51.
Maniatis T, Fritsch E F and Sambrook J 1982Molecular cloning, A laboratory manual (Cold Spring Harbor: Cold Spring Harbor Laboratory Press) p. 85
Matthews R E F 1982 Classification and nomenclature of viruses, Fourth report of the International Committee on Taxonomy of Viruses;Intervirology 17 1–199
Mclntosh A H, Rice W C and Ignoffo C M 1987 Genotypic variants in wild-type populations of baculoviruses; inBiotechnology in invertebrate pathology and cell culture (ed.) K Maramorosch (London: Academic Press) pp 305–321
Miller D W and Miller L K 1982 A virus mutant with an insertion of a copia-like transposable element;Nature (London) 299 562–564
Miller L K 1984 Exploring the genome organization of baculoviruses; inMethods in virology (eds) K Maramorosch and H Koprowski (New York: Academic Press) voI7, pp 227–258
Mohan K S 1990Isolation and characterization of Oryctes baculovirus (Indian isolate, KI) and its use in the biological control of the insect pest Oryctes rhinoceros (L.), Ph.D. thesis, Indian Institute of Science, Bangalore
Mohan K S and Gopinathan K P 1989a Characterization of viral proteins ofOryctes baculovirus and comparison between two geographical isolates;Arch. Virol. 109 207–222
Mohan K S and Gopinathan K P 1989b Quantitation of serological cross reactivity between two geographical isolates ofOryctes baculovirus by a modified ELISA;J. Virol. Methods 24 203–214
Mohan K S and Gopinathan KP 1991 Physical mapping of the genomic DNA of theOryctes rhinoceros baculovirus, KI;Gene 107 343–344
Mohan K S, Jayapal S P and Pillai G B 1983 Baculovirus diseases inOryctes rhinoceros population in Kerala;J. Plant Crops 11 154–161
Patel C V and Gopinathan K P 1987 Determination of trace amounts of 5-methylcytosine in DNA by reverse phase HPLC;Anal. Biochem. 164 164–169
Payne C C 1974 The isolation and characterization of a virus fromOryctes rhinoceros;J. Gen, Virol. 25 105–116
Purrini K 1989 BaculovirusOryctes release intoOryctes monoceros population in Tanzania, with special reference to the interaction of virus isolates used in our laboratory infection experiments;J. Invertebr. Pathol. 53 285–300
Rohrmann G F, McParland R H, Martignoni M E and Beaudreau G S 1978 Genetic relatedness of two nuclear polyhedrosis viruses pathogenic forOrgyia pesudotsugata;Virology 84 213–217
Rigby P W J, Dieckmann M, Rhodes C and Berg P 1977 Labelling deoxyribonucleic acid to high specific activityin vitro by nick translation with DNA polymerase I;J. Mol. Biol. 113 237–251
Smith IRL and Crook N E 1988In vivo isolation of baculovirus genotypes;Virology 166 240–244
Wagner G W and Paschke J D 1977 A comparison of the DNAs of the “R” and “T” strains of mosquito iridescent virus;Virology 81 298–308
Zelazny B 1977 Occurrence of the baculovirus disease of the coconut palm rhinoceros beetle in the Philippines and Indonesia;FAO Plant Prot. Bull. 25 73–77
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mohan, K.S., Gopinathan, K.P. Characterization of the genome ofOryctes baculovirus, a viral biocide of the insect pestOryctes rhinoceros . J Biosci 17, 421–430 (1992). https://doi.org/10.1007/BF02720097
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02720097