Skip to main content
SpringerLink
Log in

Interactions of carbohydrates and proteins by fluorophore-assisted carbohydrate electrophoresis

  • Brief Communication
  • Published:
Journal of Biosciences Aims and scope Submit manuscript

Abstract

A sensitive, specific, and rapid method for the detection of carbohydrate-protein interactions is demonstrated by fluorophore-assisted carbohydrate electrophoresis (FACE). The procedure is simple and the cost is low. The advantage of this method is that carbohydrate-protein interactions can be easily displayed by FACE, and the carbohydrates do not need to be purified.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Jackson P 1994 High-resolution polyacrylamide gel electrophoresis of fluorophore-labeled reducing saccharides;Methods Enzymol. 230 250–265

    Article  PubMed  CAS  Google Scholar 

  • Jackson P 1990 The use of polyacrylamide-gel electrophoresis for the high-resolution separation of reducing saccharides labelled with the fluorophore 8-aminonaphthalene-1,3,6-trisulphonic acid;Biochem. J. 270 705–713

    PubMed  CAS  Google Scholar 

  • Stack R J and Sullivan M T 1992 Electrophoretic resolution and fluorescence detection of N-linked glycoprotein oligosaccharides after reductive amination with 8-aminonaphthalene-1,3,6-trisulphonic acid;Glycobiology 2 85–92

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, Shandong, People’s Republic of China

    Gang-Liang Huang, Xin-Ya Mei & Peng-George Wang

Authors
  1. Gang-Liang Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xin-Ya Mei
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Peng-George Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gang-Liang Huang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Huang, GL., Mei, XY. & Wang, PG. Interactions of carbohydrates and proteins by fluorophore-assisted carbohydrate electrophoresis. J Biosci 31, 219–222 (2006). https://doi.org/10.1007/BF02703914

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02703914

Keywords

Search

Navigation