Abstract
By hybridizing human adult testis cDNA microarrays with human adult and embryo testis cDNA probes, we identified a novel human testis gene, PINCH 2. PINCH 2 expression was 3.4-fold higher in adult than in fetal testis. The full length of its cDNA was 963. bp, with a 354-bp open reading frame (ORF), encoding a 117-amino acid protein. PINCH 2 was a splicing isoform of PINCH. It shared one exon, which encoded the LIM domain, with PINCH gene in human genome. Multitissue reverse transcriptase-polymerase chain reaction (RTPCR) analysis revealed that this gene was expressed variably in a wide range of tissues, with high expression levels in human adult testis. These results suggest that PINCH 2, a novel LIM domain-containing gene, may play an important role in testicular development/spermatogenesis.
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Liu, Y., Liu, J., Chen, J. et al. Molecular cloning and characterization of a novel splice variant of the LIM domain family gene, PINCH 2, in human testis. Mol Biotechnol 35, 109–118 (2007). https://doi.org/10.1007/BF02686105
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DOI: https://doi.org/10.1007/BF02686105