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DNA extraction from a previously recalcitrant plant genus

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Abstract

Numerous DNA extraction methods failed to remove contaminants that interfere with restriction digests ofCuphea DNA. The method described here removes those contaminants and maintains relatively high DNA yields. The primary purification process consists of washing the DNA with phenol while it is complexed with CTAB and dissolved in 1 M NaCl.

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Abbreviations

CTAB:

hexadecyltrimethylammonium bromide

RFLP:

restriction fragment length polymorphism

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Authors and Affiliations

  1. Department of Crop Science, Oregon State University, 97331, Corvallis, Oregon, USA

    David M. Webb & Steven J. Knapp

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  1. David M. Webb
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  2. Steven J. Knapp
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Webb, D.M., Knapp, S.J. DNA extraction from a previously recalcitrant plant genus. Plant Mol Biol Rep 8, 180–185 (1990). https://doi.org/10.1007/BF02669514

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  • DOI: https://doi.org/10.1007/BF02669514

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