Summary
This paper compares different approaches, reported in the literature, for obtaining thermodynamic statistical information from ESR and NMR measurements on the conformations of lipid bilayers. A statistical approach recently proposed by the authors has been employed to reexamine several literature values of order parameters and to calculate Helmholtz free energy and entropy. This approach was applied to eggphosphatidylcholine, cerebroside and gangliosoyds, spin labelled at different acyl chain positions, and to bilayered membranes containing phosphatidylcholine and different gangliosides (GM1, GD1a and GT1b) at increasing glycolipid mole percent. The variation of the stability of the membrane with the peroxidation is also reexamined at different intramembrane locations by various probes.
Riassunto
Si confrontano diversi procedimenti di letteratura per ricavare informazioni termodinamiche statistiche da misure ESR e NMR sulle conformazioni di doppi strati lipidici. Dal riesame di numerosi dati di letteratura sui parametri d'ordine, si calcolano energia libera di Helmholtz ed entropia ricorrendo ad un approccio statistico recentemente proposto dagli autori. Si riconsiderano fosfatidilcolina di uovo, cerebroside e gangliosoidi marcati magneticamente in diverse posizioni della catena acilica, e membrane a doppio strato contenenti fosfatidilcolina e diversi gangliosidi (GM1, GD1a e GT1b) a crescenti percentuali molari. Si riesamina anche, con diverse sonde magnetiche e in diverse posizioni interne della membrana, la variazione della stabilità della membrana stessa con la perossidazione.
Резюме
В этой статье сравниваются различые подходы, имеюшиеся в литературе для получения термодинамической статистической информации из измерений электронного спинового резонанса и ядерного магнитного резонанса на конформциях липидных бислоев. Статистический подход, предложенный недавно авторами, применяется для повторного исследования некоторых литературых данных для параметров упорядоченности и для вычисления свободной энергии Гелымгольца и энтропии. Также заново исследуется изменение стабильности мембран при перекислении при различнх внутримембранных положениях различных датчиков.
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Oliva, C., Viani, P., Marchesini, S. et al. Order and membrane stability from magnetic resonance measurements: an application of statistical thermodynamics. Il Nuovo Cimento D 10, 793–803 (1988). https://doi.org/10.1007/BF02450140
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DOI: https://doi.org/10.1007/BF02450140