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Entrapment of microbial cells within polyurethane hydrogel beads with the advantage of low toxicity

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Biotechnology Techniques

Summary

A new method is described for the entrapment of microbial cells in polyurethane (PUR) hydrogel beads. This hydrogel is produced from a hydrophilic pre-polymer blocked with bisulphite by adjusting the pH between 4 and 6.5. Bisulphite-blocked isocyanate has a substantially lower toxicity against living cells than unblocked (conventional) isocyanates. The poly(carbamoylsulfonate) (PCS) hydrogels have optimal elastic properties and therefore can be used for a matrix of biocatalysts in an agitated reactor as well as in a fluid-bed reactor. The results of ethanol fermentation ofSaccharomyces cerevisiae entrapped in PCS hydrogel beads, and of the denitrification activity of immobilizedParacoccus denitrificans are promising. In contrast, entrapped cells in conventional PUR hydrogels didn’t show any activity.

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References

  • Fukui, S., Ahmed, A., Omata, T., and Tanaka, A. (1980).Eur. J. Appl. Microbiol. Biotechnol. 10, 189–201.

    Article  Google Scholar 

  • Fukui, S., Sonomoto, K., and Tanaka, A. (1987).Methods Enzymol. 135, 230–252.

    Article  CAS  Google Scholar 

  • Klein, J., and Kluge, M. (1981).Biotechnol. Lett. 3, 65–70.

    Article  CAS  Google Scholar 

  • Klein, J., and Wagner, F. (1983).Appl. Biochem. Bioeng. 4, 11–51.

    CAS  Google Scholar 

  • Petersen, S. (1949).Liebigs Ann. Chem. 562, 205–229.

    CAS  Google Scholar 

  • Schmidt, J., Beyersdorf, J., Dirks, U., Remmers, P., and Vorlop, K.-D. (1989). Development of a process for denitrification of drinking water. In:DECHEMA Biotechnology conferences, D. Behrens and A.J. Driesel, eds. Vol. 3, pp. 833–836, Weinheim: VCH.

    Google Scholar 

  • Spiekermann, P., Vorlop, K.-D., and Klein, J. (1987). Animal cells encapsulated within Ca-alginate hollow-spheres. In:Proc. 4th Eur. Congr. Biotechnol., O.M. Neijssel, R.R. Van der Meer and K.Ch.A.M. Luyben, eds. Vol. 3, pp. 590–593. Amsterdam: Elsevier Science Publishers.

    Google Scholar 

  • Vorlop, K.-D., Becke, J.W., and Klein, J. (1983).Biotechnol. Lett. 8, 509–514.

    Article  Google Scholar 

  • Vorlop, K.-D., and Klein, J. (1983). New development in the field of cell immobilization — formation of biocatalysts by ionotropic gelation. In:Enzyme Technology, R.M. Lafferty, ed., pp. 219–235, Berlin: Springer Verlag.

    Google Scholar 

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Authors and Affiliations

  1. Technische Chemie, Univ. Oldenburg, Ammerländer Heerstr. 114-118, D-W-2900, Oldenburg

    Klaus-Dieter Vorlop & Andreas Muscat

  2. Institut für Technische Chemie, TU Braunschweig, Hans-Sommer-Str. 10, D-W-3300, Braunschweig

    Jörg Beyersdorf

Authors
  1. Klaus-Dieter Vorlop
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  2. Andreas Muscat
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  3. Jörg Beyersdorf
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Vorlop, KD., Muscat, A. & Beyersdorf, J. Entrapment of microbial cells within polyurethane hydrogel beads with the advantage of low toxicity. Biotechnol Tech 6, 483–488 (1992). https://doi.org/10.1007/BF02447818

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  • DOI: https://doi.org/10.1007/BF02447818

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