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A new RNA picorna — Like virus in the cotton pink bollwormPectinophora gossypiella (Lep.: Gelechiidae) in Egypt

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Abstract

A new virus infecting the pink bollwormPectinophora gossypiella has been detected and purified from dead larvae collected from naturally infested cotton fields. The purified icosahedric virions measured 27±1 nm in diameter and contained RNA genome. Three capsid proteins of 31.7, 32.6 and 47.4 Kd have been separated on polyacrylamide gel. The purified virus was not highly infectious to the host larvae revealed while the pupal period survived from infected larvae was significantly prolonged. The virus particles infecting the midgut cells are grouped in paracrystallin arrays. The virus was vertically transmitted through infected adults. The main characteristics of this virus place are quite relative to the Picornavirus group.

Résumé

Un nouveau virus infectant le ver rose du cotonnier,Pectinophora gossypiella, a été isolé et purifié à partir de larves mortes récoltées en champ de coton. Les virions purifiés de 27±1 nm de diamètre, ont une forme icosaédrique et contiennent un génome à ARN. Trois protéines capsidaires de 31,7, 32,6 et 47,4 Kd ont été trouvées dans les virions. Le virus purifié n'est pas hautement infectieux pour les larves, cependant la durée du stade chrysalide est supérieure chez les lots infectés artificiellement. Dans les tissues atteints, les virions sont dispersés dans le cytoplasme des cellules de l'intestin ou bien groupés en amas paracrystallins de même type que chez d'autres insectes infectés avec des picornavirus. Les adultes malades transmettent ce virus à leur descendance. Par sa forme, sa taille et ses caractéristiques chimiques, ce virus libre peut être placé près des Picornavirus d'insectes.

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Authors and Affiliations

  1. French /Egyptian Virology Lab., Fac. of Agriculture, Cairo University, Giza, Egypt

    A. Monsarrat, S. Abol-Ela, I. Abdel-Hamid, G. Fediere & J. Giannotti

  2. Station de Recherches INRA-CNRS, 30380, St Christol-les-Alès, France

    G. Kuhl

  3. Biological Control Lab., Fac. of Agriculture, Cairo University, Giza, Egypt

    M. El Husseini

Authors
  1. A. Monsarrat
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  2. S. Abol-Ela
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  3. I. Abdel-Hamid
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  4. G. Fediere
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  5. G. Kuhl
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  6. M. El Husseini
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  7. J. Giannotti
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Monsarrat, A., Abol-Ela, S., Abdel-Hamid, I. et al. A new RNA picorna — Like virus in the cotton pink bollwormPectinophora gossypiella (Lep.: Gelechiidae) in Egypt. Entomophaga 40, 47–54 (1995). https://doi.org/10.1007/BF02372680

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  • DOI: https://doi.org/10.1007/BF02372680

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