Abstract
Gonadotropin-releasing hormone (GnRH), having a highly conserved structure across mammalian species, plays a pivotal role in the control of the neuroendocrine events and the inherent sexual behaviors essential for reproductive function. Recent advances in molecular genetic technology have contributed greatly to the investigation of several aspects of GnRH physiology, particularly steroid hormone and neurotransmitter regulation of GnRH gene expression. Behavioral studies have focused on the actions of GnRH in steroid-sensitive brain regions to understand better its role in the facilitation of mating behavior. To date, however, there are no published reports which directly correlate GnRH gene expression and reproductive behavior. The intent of this article is to review the current understanding of the way in which changes in GnRH gene expression, and modifications of GnRH neuronal activity, may ultimately influence reproductive behavior.
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Ackland, J. F., Nikolics, K., Seeburg, P. H., and Jackson, I. M. D. (1988). Molecular forms of gonadotropin-releasing hormone associated peptide (GAP): Changes within the rat hypothalamus and release from hypothalamic cellsin vitro.Neuroendocrinology 48:376–386.
Adams, L. A., and Steiner, R. A. (1988). Puberty.Oxf. Rev. Reprod. Biol. 10:1–52.
Adelman, J. P., Mason, A. J., Hayflick, J. S., and Seeburg, P. H. (1986). Isolation of the gene and hypothalamic cDNA for the common precursor of gonadotropin-releasing hormone and prolactin release-inhibiting factor in human and rat.Proc. Natl. Acad. Sci. USA 83:179–183.
Ahima, R. S., and Harlan, R. E. (1992). Glucocorticoid receptors in LHRH neurons.Neuroendocrinology 56:845–850.
Ambach, G., Kivovics, P., and Palkovits, M. (1978). The arterial and venous blood supply of the preoptic region in the rat.Acta Morph. Hung. 26:21–41.
Amoss, M., Burges, R., Blackwell, R., Vale, W., Fellows, R., and Gulliemin, R. (1971). Purification, amino acid composition and n-terminus of the hypothalamic luteinizing hormone releasing hormone factor (LRF) of ovine origin.Biochem. Biophys. Res. Commun. 44:205–210.
Badger, T. M., Wilcox, C. E., Meyer, E. R., Bell, R. D., and Cicero, T. J. (1978). Simultaneous changes in tissue and serum levels of luteinizing hormone, follicle-stimulating hormone, and luteinizing hormone/follicle-stimulating hormone releasing factor after castration in the male rat.Endocrinology 102:136–141.
Badr, M., and Pelletier, G. (1987). Characterization and autoradiographic localization of LHRH receptors in the rat brain.Synapse 1:567–571.
Barraclough, C. A., and Wise, P. M. (1982). The role of catecholamines in the regulation of pituitary luteinizing hormone and follicle-stimulating hormone secretion.Endocrine Rev. 3:91–119.
Barraclough, C. A., Wise, P. M., and Selmanoff, M. K. (1984). A role for hypothalamic catecholamines in the regulation of gonadotropin secretion.Rec. Prog. Horm. Res. 40:486–529.
Barry, J. (1979). Immunohistochemistry of luteinizing hormone releasing hormone-producing neurons of the vertebrates.Int. Rev. Cytol. 60:179–219.
Barry, J., Hoffman, G. E., and Wray, S. (1985). LHRH-containing systems.Hand. Chem. Neuroanat. 4:166–215.
Baulieu, E. E. (1991). Neurosteroids: A function of the brain. In Costa, E., and Paul, S. M. (eds.)Neurosteroids and Brain Function, Fidia Res. Found. Symp.8:63–76.
Beauvillain, J. C., Tramu, G., and DuBois, M. P. (1981). Ultrastructural immunocytochemical evidence of the presence of a peptide related to ACTH in granules of LHRH nerve terminals in the median eminence of the guinea pig.Cell Tissue Res. 218:1–6.
Becker, I. I., Jakubowski, M., Tsutsumi, M., Flanagan, C. A., Sealfon, S., Millar, R. P., and Dutlow, C. M. (1994). GnRH receptor mRNA, GnRH and GnRH mRNA responses to castration and testosterone replacement in the male rat.Endocrine Soc. Abstr. 1185, p. 497.
Bennett-Clarke, C., and Joseph, S. A. (1982). Immunocytochemical distribution of LHRH neurons and processes in the rat: Hypothalamic and extrahypothalamic locations.Cell Tissue Res. 221:493–504.
Bergen, H. T., Hejtmancik, J. F., and Pfaff, D. W. (1991). Effects of gamma-aminobutyric acid receptor agonists and antagonist on LHRH-synthesizing neurons as detected by immunocytochemistry andin situ hybridization.Exp. Brain Res.87:46–56.
Beyer, C., Gomora, P., Canchola, E., and Sandoval, Y. (1982). Pharmacological evidence that LH-RH action on lordosis behavior is mediated through a rise in cAMP.Horm. Behav. 16:107–112.
Bond, C. T., Hayflick, J. S., Seeburg, P. H., and Adelman, J. P. (1989). The rat gonadotropin-releasing hormone SH locus: Structure and hypothalamic expression.Mol. Endocrinol. 3:1257–1262.
Brar, A. K., McNeilly, A. S., and Fink, G. (1985). Effects of hyperprolactinemia and testosterone on the release of LH-releasing hormone and the gonadotropins in intact and castrated rats.J. Endocrinol. 104:35–43.
Brown, D., Herbison, A. E., Robinson, J. E., Marrs, R. W., and Leng, G. (1994). Modelling the luteinizing hormonereleasing hormone pulse generator.Neuroscience 63:869–879.
Buma, P. (1989). Characterization of luteinizing hormone-releasing hormone fibers in the mesencephalic central grey substance of the rat.Neuroendocrinology 49:623–630.
Burbach, J. P. H., DeHoop, M. J., Schmale, H., Richter, D., de Kloet, E. R., Ten Haaf, J. A., and de Wied, D. (1984). Differential responses to osmotic stress of vasopressin-neurophysin mRNA in hypothalamic nuclei.Neuroendocrinology 39:582–584.
Caraty, A., and Locatelli, A. (1988). Effect of time after castration on secretion of LH-RH and LH in the ram.J. Reprod. Fert. 82:263–269.
Caraty, A., Locatelli, A., and Martin, G. B. (1989). Biphasic response in the secretion of gonadotropin-releasing hormone in ovariectomized ewes injected with estradiol.J. Endocrinol. 123:374–382.
Carmel, P. W., Araki, S., and Ferin, M. (1976). Pituitary stalk portal blood collection in rhesus monkeys: Evidence for pulsatile release of gonadotropin-releasing hormone (GnRH).Endocrinology 99:243–248.
Cattanach, H. M., Iddon, C. A., Charlton, H. M., Chiappa, S. A., and Fink, G. (1977). Gonadotropin-releasing hormone deficiency in a mutant mouse with hypogonadism.Nature 269:338–340.
Chan-Palay, V., and Palay S. (eds.) (1984).Coexistence of Neuroactive Substances in Neurons, John Wiley and Sons, New York.
Chandran, U. R., Attardi, B., Friedman, R., Dong, K. W., Roberts, J. L., and DeFrance, D. B. (1994). Glucocorticoid receptor mediated repression of GnRH gene promoter activity in GT1 hypothalamic cell lines.Endocrinology 134: 1467–1474.
Charlton, H. M. (1987). Putting genes into hypogonadal mice.Trends Neurosci. 10:229–231.
Charnay, Y., Bouras, C., Vallet, P. G., Golaz, J., Guntern, R., and Constantinidis, J. (1989). Immunohistochemical colocalization of DSIP and LHRH in rabbit brain neurons.Neuroscience 31:495–505.
Chen, W.-P., Witkin, J. W., and Silverman, J.-A. (1989a). Gonadotropin releasing hormone (GnRH) neurons are directly innervated by catecholamine terminals.Synapse 3: 288–290.
Chen, W.-P., Witkin, J. W., and Silverman, J.-A. (1989b). Beta-endorphin and gonadotropin-releasing hormone synaptic input to gonadotropin-releasing hormone neurosecretory cells in the male rat.J. Comp. Neurol. 286: 85–95.
Chen, W. P., Witkin, J. W., and Silverman, A. J. (1990). Sexual dimorphism in the synaptic input to gonadotropin releasing hormone neurons.Endocrinology 126:695–702.
Childs, G. V. (1990). Localization of gonadotropin-releasing hormone receptors.Meth. Enzymol. 184:395–404.
Clarke, I. J., and Cummins, J. T. (1982). The temporal relationship between gonadotropin releasing hormone (GnRH) and luteinizing hormone (LH) secretion in ovariectomized ewes.Endocrinology 111:1737–1739.
Clayton, R. N. (1989). Gonadotropin-releasing hormone: its actions and receptors.J. Endocrinol. 120:11–19.
Clayton, R. N., Detta, A., Naik, S. I., Young, S., and Charlton, H. M. (1985). Gonadotropin-releasing hormone receptor regulation in relationship to gonadotropin secretion.J. Steroid Biochem. 23:691–702.
Coen, C. W., Montagnese, C., and Opacka-Juffry, J. (1990). Coexistence of gonadotropin-releasing hormone and galanin: Immunohistochemical and functional studies.J. Neuroendocrinol. 2:107–111.
Comb, M., Hyman, S. E., and Goodman, H. M. (1987). Mechanisms of trans-synaptic regulation of gene expression.Trends Neurosci. 10:473–478.
Conn, P. M. (1994). The molecular mechanism of gonadotropin-releasing hormone action in the pituitary. In Knobil, E., and Neill, J. D., et al. (eds.),The Physiology of Reproduction, 2nd ed., Raven Press, New York, pp. 1815–1832.
Conn, P. M., Huckle, W. R., Andrews, W. V., and McArdle, C. A. (1987a). The molecular mechanism of action of gonadotropin releasing hormone (GnRH) in the pituitary.Rec. Prog. Horm. Res. 43:29–68.
Conn, P. M., McArdle, C. A., Andrews, W. V., and Huckle, W. R. (1987b). The molecular basis of gonadotropin-releasing hormone (GnRH) action in the pituitary gonadotrope.Biol. Reprod. 36:17–35.
Connor, J. P., Wadman, W. J., Hockberger, P. E., and Wong, R. K. S. (1988). Sustained dendritic gradient of Ca2+ induced by excitatory amino acids in CA1 hippocampal neurons.Science 240:649–653.
Costa, E., and Neff, N. H. (1977). Estimation of turnover rates to study the metabolic regulation of the steady state level of neural amines.Hand. Neurochem. 4:45–90.
Cramer, O. M., and Barraclough, C. A. (1975). Failure to detect luteinizing hormone-releasing hormone in third ventricle cerebral spinal fluid under a variety of experimental conditions.Endocrinology 96:913–21.
Crighton, D. B., Schneider, H. P. G., and McCann, S. M. (1970). Localization of LH-releasing factor in the hypothalamus and neurohypophysis as determined by anin vitro method.Endocrinology 87:323–329.
Culler, M. D., Valenca, M. M., Merchenthaler, I., Flerkó, B., and Negro-Vilar, A. (1988). Orchidectomy induces temporal and regional changes in the processing of the luteinizing hormone-releasing hormone prohormone in the rat brain.Endocrinology 122:1968–1976.
de Beun, R., Geerts, N. E., Vreeburg, J. T. M., Slangen, J. L., and van de Poll, N. E. (1991). Luteinizing hormone-releasing hormone-induced conditioned place preference in male rats.Pharmacol. Biochem. Behav. 39:143–147.
de Beun, R., Heinsbroek, R. P. W., Slangen, J. L., and van de Poll, N. E. (1992). Dose-dependent and time-related stimulus properties of LHRH in male but not female rats.Psychoneuroendocrinology 17:71–79.
Denenberg, V. H. (1984). Some statistical and experimental considerations in the use of the analysis-of-variance procedure.Am. J. Physiol. 246:R403-R408.
DePaolo, L. V., and Barraclough, C. A. (1979). Interactions of estradiol and progesterone on pituitary gonadotropin secretion: Possible sites and mechanisms of action.Biol. Reprod. 20:1173–1179.
Dluzen, D. E., and Ramirez, V. D. (1985).In vivo activity of the LH-releasing hormone pulse generator in castrated and intact male rats.J. Endocrinol. 107:331–340.
Dluzen, D. E., and Ramirez, V. D. (1987).In vivo activity of the LHRH pulse generator as determined with push-pull perfusion of the anterior pituitary gland of unrestrained intact and castrate male rats.Neuroendocrinology 45:328–332.
Dornan, W. A., and Malsbury, C. W. (1989). Neuropeptides and male sexual behavior.Neurosci. Biobehav. Rev. 13: 1–15.
Dorsa, D. M., and Smith, E. R. (1980). Facilitation of mounting behavior in male rats by intracranial injections of luteinizing hormone releasing hormone.Peptides 1:147–155.
Dorsa, D. M., Smith, E. R., and Davidson, J. M. (1981). Endocrine and behavioral effects of continuous exposure of male rats to potent luteinizing hormone-releasing hormone (LHRH) agonist: Evidence for central nervous system actions of LHRH.Endocrinology 109:729–735.
Drouva, S. V., Gautron, J. P., Pattou, E., Laplante, E., and Kordon, C. (1986). Effects of estradiol and progesterone on immunoreactive forms of hypothalamic luteinizing hormone-releasing hormone.Neuroendocrinology 43:32–37.
Dudley, C. A., and Moss, R. L. (1988). Facilitation of lordosis in female rats by CNS-site specific infusions of an LHRH fragment, Ac-LH-RH-(5–10).Brain Res. 441:161–167.
Dudley, C. A., and Moss, R. L. (1991). Facilitation of sexual receptivity in the female rat by C-terminal fragments of LHRH.Physiol. Behav. 50:1205–1208.
Dudley, C. A., Vale, W., Rivier, J., and Moss, R. L. (1981). The effect of LHRH antagonists analogs and an antibody to LHRH on mating behavior in female rats.Peptides 2: 393–396.
Dutlow, C. M., Rachman, J., Jacobs, T. W., and Millar, R. P. (1992). Prepubertal increases in gonadotropin-releasing hormone messenger RNA, gonadotropin-releasing hormone precursor, and subsequent maturation.J. Clin. Invest. 90:2496–2501.
Dyer, R. G., and Robinson, J. E. (1989). The LHRH pulse generator.J. Endocrinol. 123:1–2.
Eidne, K. A., Sellar, R. F., Couper, G., Anderson, L., and Taylor, P. L. (1992). Molecular cloning and characterization of the rat pituitary gonadotropin-releasing hormone (GnRH) receptor.Mol. Cell. Endocrinol. 90:85–89.
Ellis, G. B. (1982). How fast does testosterone feed back on LH secretion?Soc. Study Reprod. Abstr. 32 26:52A.
Ellis, G. B., and Desjardins, C. (1984). Orchidectomy unleashes pulsatile luteinizing hormone secretion in the rat.Biol. Reprod. 30:619–627.
Eskay, R. L., Mical, R. S., and Porter, J. C. (1977). Relationship between luteinizing hormone releasing hormone concentration in hypophysical portal blood and luteinizing hormone release in intact, castrated and electrochemically-stimulated rats.Endocrinology 100:263–270.
Everett, J. W. (1994). Pituitary and hypothalamus: Perspectives and overview. In Knobil, E., and Neill, J. D. et al. (eds.),The Physiology of Reproduction, 2nd ed., Raven Press, New York, pp. 1509–1526.
Fallest, P. C., Hiatt, E. S., and Schwartz, N. B. (1989). Effects of gonadectomy on thein vitro andin vivo gonadotropin responses to gonadotropin-releasing hormone in male and female rats.Endocrinology 124:1370–1379.
Favit, A., Wetsel, W. C., and Negro-Vilar, A. (1993). Differential expression of γ-aminobutyric acid receptors in immortalized luteinizing hormone-releasing hormone neurons.Endocrinology 133:1983–1989.
Findell, P. R., Wong, K. H., Jackman, J. K., and Daniels, D. V. (1993). α1 and dopamine (D1)-receptors coupled to adenylyl cyclase activation in GT1 gonadotropin-releasing hormone neurosecretory cells.Endocrinology 132:682–688.
Fink, G., Sheward, W. J., and Charlton, H. M. (1982). Priming effect of luteinizing hormone releasing hormone in the hypogonadal mouse.J. Endocrinol. 94:283–287.
Flügge, G., Oertel, W. H., and Wuttke, W. (1986). Evidence for estrogen receptive GABAergic neurons in the preoptic/anterior hypothalamic area of the rat brain.Neuroendocrinology 43:1–5.
Fox, S. R., Harlan, R. E., Shivers, B. D., and Pfaff, D. W. (1990). Chemical characterization of neuroendocrine targets for progesterone in the female rat brain and pituitary.Neuroendocrinology 51:276–283.
Franco, B., Guioli, S., Pragliola, A., Incerti, B., Bardoni, B., Tonlorenzi, R., Carrozzo, R., Maestrini, E., Pieretti, M., Taillon-Miller, P., Brown, C. J., Willard, H. F., Lawrence, C., Persico, M. G., Camerino, G., and Ballabio, A. (1991). A gene deleted in Kallmann's syndrome shares homology with neural cell adhesion and axonal path-finding molecules.Nature 353:529–536.
Freeman, M. E., Dupke, K. C., and Croteau, C. M. (1976). Extinction of the estrogen-induced daily signal for LH release in the rat: A role for the proestrous surge of progesterone.Endocrinology 99:223–229.
Fuxe, K., and Agnati, L. F. (1991).Volume Transmission in the Brain: Novel Mechanisms for Neural Transmission, Raven Press, New York.
Gargiulo, P. A., and Donoso, A. O. (1989). Luteinizing hormone releasing hormone (LHRH) in the periaqueductal gray substance increases some subcategories of grooming behavior in male rats.Pharmacol. Biochem. Behav. 32: 853–856.
Gargiulo, P. A., Muñoz, V., and Donoso, A. O. (1992). Inhibition by N-methyl-D-aspartic acid (NMDA) receptor antagonist of lordosis behavior induced by estrogen followed by progesterone or luteinizing hormone-releasing hormone (LHRH) in the rat.Physiol. Behav. 52:737–739.
Gibson, M. J., and Silverman, A. J. (1989). Effects of gonadectomy and treatment with gonadal steroids on luteinizing hormone secretion in hypogonadal male and female mice with preoptic area implants.Endocrinology 125: 1525–1532.
Gibson, M. J., Krieger, D. T., Charlton, H. M., Zimmerman, E. A., Silverman, A. J., and Perlow, M. J. (1984a). Mating and pregnancy can occur in genetically hypogonadal mice with preoptic area brain grafts.Science 225:949–951.
Gibson, M. J., Perlow, M. J., Charlton, H. M., Zimmerman, E. A., Davies, T. F., and Krieger, D. T. (1984b). Preoptic area brain grafts in hypogonadal (hpg) female mice abolish effects of congenital hypothalamic gonadotropin releasing hormone (GnRH) deficiency.Endocrinology 114: 1938–1940.
Gibson, M. J., Moscovitz, H. C., Kokoris, G. J., and Silverman, A. J. (1987). Plasma LH rises rapidly following mating in hypogonadal female mice with preoptic area (POA) brain grafts.Brain Res. 424:133–138.
Gibson, M. J., Kokoris, G. J., and Silverman, A. J. (1988). Positive feedback in hypogonadal female mice with preoptic area brain transplants.Neuroendocrinology 48: 112–119.
Gibson, M. J., Silverman, A. J., Rosenthal, M. F., and Morrell, J. I. (1989). Estradiol-concentrating cells in the brains of hypogonadal female mice and in their intraventricular preoptic area implants.Exp. Neurol. 105:127–134.
Glowinski, J. (1975). Properties and functions of intraneuronal monoamine compartments in central aminergic neurouns.Hand. Psychopharmacol. 3:139–167.
Goldsmith, P. C. and Ganong, W. F. (1975). Ultrastructural localization of luteinizing hormone-releasing hormone in the median eminence of the rat.Brain Res. 97:181–193.
Goldsmith, P. C., Thind, K. K., Perera, A. D., and Plant, T. M. (1994). Glutamate-immunoreactive neurons and their gonadotropin-releasing hormone-neuronal interactions in the monkey hypothalamus.Endocrinology 134:858–868.
González-Mariscal, G., and Beyer, C. (1989). Blockade of LHRH-induced lordosis by α− and β-adrenergic antagonists in ovariectomized, estrogen primed rats.Pharmacol. Biochem. Behav. 31:573–577.
Gore, A. C., and Roberts, J. L. (1994). Regulation of gonadotropin-releasing hormone gene expression by the excitatory amino acids kainic acid andN-methyl-D, L-aspartate in the male rat.Endocrinology 134:2026–2031.
Gore, A. C., and Roberts, J. L. (1995). Regulation of gonadotropin-releasing hormone gene expression in the rat during the luteinizing hormone surge.Endocrinology 136: 889–896.
Gorski, R. A. (1979). The neuroendocrinology of reproduction: An overview.Biol. Reprod. 20:111–127.
Grattan, D. R., and Selmanoff, M. (1993). Regional variation in γ-aminobutyric acid turnover: Effect of castration on γ-aminobutyric acid turnover in microdissected brain regions of the male rat.J. Neurochem. 60:2254–2264.
Grattan, D. R., and Selmanoff, M. (1994a). Castration-induced decrease in the activity of medial preoptic and tuberoinfundibular GABAergic neurons is prevented by testosterone.Neuroendocrinology 59:141–149.
Grattan, D. R., and Selmanoff, M. (1994b). Prolactin- and testosterone-induced inhibition of LH secretion after orchidectomy: Role of preoptic and tuberoinfundibular γ-aminobutyric acidergic neurones.J. Endocrinol 143: 165–174.
Grattan, D. R., Park, S.-K., and Selmanoff, M. (1995). Orchidectomy and NMDA increase GnRH secretion as measured by push-pull perfusion of rat anterior pituitary.Am. J. Physiol. 268:E685-E692.
Graves, R. A., Pandey, N. B., Chodchoy, N., and Marzluff, W. F. (1987). Translation is required for regulation of histone mRNA degradation.Cell 48:615–626.
Gruenewald, D. A., and Matsumoto, A. M. (1991). Age-related decreases in serum gonadotropin levels and gonadotropinreleasing hormone gene expression in the medial preoptic area of the male rat are dependent upon testicular feedback.Endocrinology 129:2442–2450.
Hales, T. G., Kim, H., Longoni, O., Olsen, R. W. and Tobin, A. J. (1992). Immortalized hypothalamic GT1-7 neurons express functional γ-aminobutyric acid type A receptors.Mol. Pharmacol. 42:197–202.
He, J.-R., and Barraclough, C. A. (1992). Morphine but not naloxone enhances luteinizing hormone-releasing hormone neuronal responsiveness to norepinephrine.J. Neurondocrinol. 4:92–99.
He, J.-R., Molnar, J., and Barraclough, C. A. (1993). Morphine amplifies norepinephrine (NE)-induced LH release but blocks NE-stimulated increased in LHRH mRNA levels: comparison of responses obtained in ovariectomized, estrogen-treated normal and androgen-sterilized rats.Mol. Brain Res. 20:71–78.
Herbison, A. E., and Theodosis, D. T. (1992). Localization of oestrogen receptors in preoptic neurons containing neurotensin but not tyrosine hydroxylase, cholecystokinin or luteinizing hormone-releasing hormone in the male and female rat,Neuroscience 50:283–298.
Herbison, A. E., Heavens, R. P., Dye, S., and Dyer, R. G. (1991). Acute action of oestrogen on medial preoptic gamma-aminobutyric acid neurons: Correlation with oestrogen negative feedback on luteinizing hormone secretion.J. Neuroendocrinol. 3:101–106.
Herbison, A. E., Robinson, J. F., and Skinner, D. C. (1993). Distribution of estrogen receptor-immunoreactive cells in the preoptic area of the ewe: Co-localization with glutamic acid decarboxylase but not luteinizing hormone-releasing hormone.Neuroendocrinology 57:751–759.
Herbison, A. E., Horvath, T. L., Naftolin, F., and Léránth, C. (1995a). Distribution of estrogen receptor-immunoreactive cells in monkey hypothalamus: relationship to neurones containing luteinizing hormone-releasing hormone and tyrosine hydroxylase.Neuroendocrinology 61:1–10.
Herbison, A. E., King, I. S., Tan, K. C., and Dye, S. (1995b). Increased fos expression in preoptic calcitonin gene-related peptide (CGRP) neurons following mating but not the luteinizing hormone surge in female rats.J. Neuroendocrinol. 7:377–385.
Hiatt, E. S., Brunetta, P. G., Seiler, D. R., Barney, S. A., Selles, W. D., Wooledge, K. H., and King, J. C. (1992). Subgroups of luteinizing hormone-releasing hormone perikarya defined by computer analyses in the basal forebrain of intact female rats.Endocrinology 130:1030–1043.
Higuchi, T., and Kawakami, M. (1982). Luteinizing hormone responses to repeated injections of luteinizing hormone releasing hormone in the rat during the oestrous cycle and after ovariectomy with or without oestrogen treatment.J. Endocrinol. 93:161–168.
Hileman, S. M., Petersen, S. L., Lubbers, L. S., Scott, C. J., Kuehl, D. E., and Jackson, G. L. (1994). Testosterone (T) reduces LHRH release without affecting LHRH mRNA.Soc. Study Reprod. Abstr. 519 50 (Suppl.):184.
Hisano, S., Kawano, H., Maki, YI., and Daikoku, S. (1981). Electron microscopic study of immunoreactive perikarya with special reference to neuronal regulation.Cell Tissue Res. 220:511–518.
Hoffman, A. R., and Crowley, W. F., Jr. (1982). Induction of puberty in men by long-term pulsatile administration of low-dose gonadotropin-releasing hormone.N. Engl. J. Med. 307:1237–1241.
Hoffman, G. E., and Gibbs, F. P. (1982). LHRH pathways in rat: “Deafferentation” spares a sub-chiasmatic LHRH projection to the median eminence.Neuroscience 7:1979–1993.
Hökfelt, T., Elde, R., Fuxe, K., Johansson, O., Ljungdahl, Å., Goldstein, M., Luft, R., Efendic, S., Nilsson, G., Terenius, L., Ganten, D., Jeffcoate, S. L., Rehfeld, J., Said, S., Perez de la Mora, M., Possani, L., Tapia, R., Teran, L., and Palacios, R. (1978). Aminergic and peptidergic pathways in the nervous system with special reference to the hypothalamus. In Reichlin, S., Baldessarini, R. J., and Martin, J. B. (eds.),The Hypothalamus, Raven Press, New York, pp. 69–136.
Horvath, T. L., Naftolin, F., and Léránth, C. (1993). Luteinizing hormone-releasing hormone and gamma-aminobutyric acid neurons in the medial preoptic area are synaptic targets of dopamine axons originating in anterior periventricular areas.J. Neuroendocrinol. 5:71–79.
Hsu, C., Lee, J.-N., Ho, M.-L., Cheng, B.-H., Li, P.-H. S., and Yu, J. Y.-L. (1993). The facilitatory effect of N-methyl-d-aspartate on sexual receptivity in female rats through GnRH release.Acta Endocrinol. 128:385–388.
Huang, X., and Harlan, R. E. (1993). Absence of androgen receptors in LHRH immunoreactive neurons.Brain Res. 624:309–311.
Iddon, C. A., Charlton, H. M., and Fink, G. (1980). Gonadotrophin release in hypogonadal and normal mice after electrical stimulation of the median eminence or injection of luteinizing hormone releasing hormone.J. Endocrinol. 85:105–110.
Jackson, G. L., Kuehl, D., and Rhim, T. J. (1991). Testosterone inhibits gonadotropin-releasing hormone pulse frequency in the male sheep.Biol. Reprod. 45:188–194.
Jakubowski, M., Blum, M., and Roberts, J. L. (1991). Postnatal development of gonadotropin-releasing hormone and cyclophilin gene expression in the female and male rat brain.Endocrinology 128:2702–2708.
Jennes, L., and Conn, P. M. (1992). Gonadotropin releasing hormone receptors in rat brain.Hand. Chem. Neuroanat. 11:223–246.
Jennes, L., and Conn, P. M. (1994). Gonadotropin releasing hormone and its receptors in rat brain.Front. Neuroendocrinol. 15:51–77.
Jennes, L., and Stumpf, W. E. (1980). LHRH neuronal projections to the inner and outer surfaces of the brain.Neuroendo. Lett. 2:241–246.
Jennes, L., and Woolums, S. (1994). Localization of gonadotropin releasing hormone receptor mRNA in rat brain.Endocrine 2:521–528.
Jennes, L., Stumpf, W. E., and Sheedy, M. E. (1985). Ultrastructural characterization of gonadotropin-releasing hormone (GnRH)-producing neurons.J. Comp. Neurol. 232: 534–547.
Jennes, L., Dalati, B., and Conn, P. M. (1988). Distribution of gonadotropin releasing hormone agonist binding sites in the rat central nervous system.Brain Res. 452: 156–164.
Kaiser, U. B., Zhao, D., Cardona, G. R., and Chin, W. W. (1992). Isolation and characterization of cDNAs encoding the rat pituitary gonadotropin-releasing hormone receptor.Biochem. Biophys. Res. Commun. 189: 1645–1652.
Kalra, S. P., and Kalra, P. S. (1983). Neural regulation of luteinizing hormone secretion in the rat.Endocrine Rev. 4: 311–351.
Kalra, S. P., and Kalra, P. S. (1989). Do testosterone and estradiol-17β enforce inhibition or stimulation of luteinizing hormone-releasing hormone secretion?Biol. Reprod. 41: 559–570.
Kalra, P. S., Simpkins, J. W., and Kalra, S. P. (1984). Testosterone raises LHRH levels exclusively in the median eminence of castrated rats.Neuroendocrinology 39: 45–48.
Karaviti, L. F., and Angelides, K. J. (1993). Expreession of progesterone and dopamine receptors on GnRH neurons.Endocrine Soc. Abstr. 955, p. 289.
Kelley, M. R., Jurgens, J. K., LaPaglia, N., and Emanuele, N. V. (1993). differential gene expression of the luteinizing hormone-releasing hormone (LHRH) gene in hypothalamic and extrahypothalamic brain regions following gonadectomy.Endocrine Soc. Abstr., 1798, p. 500.
Kelly, M. J. (1982). Electrical effects of steroids in neurons. In McKerns, K. W., and Pantic, V. (eds.).Hormonally Active Brain Peptides: Structure and Function, Plenum Press, New York, pp. 253–277.
Kelly, M. J., Garrett, J., Bosch, M. A., Roselli, C. E., Douglass, J., Adelman, J. P., and Ronnekleiv, O. K. (1989). Effects of ovariectomy on GnRH mRNA, proGnRH and GnRH levels in the preoptic hypothalamus of the female rat.Neuroendocrinology 49: 88–97.
Kepa, J. K., Wang, C., Neeley, C. I., Raynolds, M. V., Gordon, D. F., Wood, W. M., and Wierman, M. E. (1992). Structure of the rat gonadotropin releasing hormone (rGnRH) gene promoter and functional analysis in hypothalamic cells.Nucleic Acids Res. 20: 1393–1399.
Khodr, G. S., and Siler-Khodr, T. M. (1980). Placental luteinizing hormone-releasing factor and its synthesis.Science 207: 315–317.
Kim, K., Lee, B. J., Park, Y., and Cho, W. K. (1989). Progesterone increases messenger ribonucleic acid (mRNA) encoding luteinizing hormone releasing hormone (LHRH) level in the hypothalamus of ovariectomized estradiolprimed prepubertal rats.Mol. Brain Res. 6: 151–158.
Kim, K., Lim, I. S., Cho, B. N., Kang, S. S., Lee, B. J., Choi, K. H., Chung, C. H., Lee, C. C., Cho, W. K., and Wuttke, W. (1993). A partial blockade of catecholaminergic neurotransmission with 6-hydroxydopamine decreased mRNA level of gonadotropin releasing hormone in the male rat hypothalamus.Neuroendocrinology 58: 146–152.
King, J. C., and Seiler, G. R. (1988). Ultrastructural evidence suggests variations in biosynthesis and processing within LH-RH neurons as a function of ovariectomy in rats.Brain Res. 452: 127–140.
King, J. C., Williams, T. H., and Arimura, A. A. (1975). Localization of luteinizing hormone-releasing hormone in rat hypothalamus using radioimmunoassay.J. Anat. 120: 275–288.
King, J. C., Anthony, E. L. P., Damassa, D. A., and Elkind-Hirsch, K. E. (1987a). Morphological evidence that luteinizing hormone-releasing hormone neurons participate in the suppression by estradiol of pituitary luteinizing hormone secretion in ovariectomized rats.Neuroendocrinology 45: 1–13.
King, J. C., Kugel, G., Zahniser, D., Wooledge, K., Damassa, D. A., and Alexsavich G. (1987b). Changes in populations of LHRH-immunopositive cell bodies following gonadectomy.Peptides 8: 721–735.
King, J. C., Tai, D. W., Hanna, I. K., Pfeiffer, A., Haas, P., Ronsheim, P. M., Mitchell, P. M., Turcotte, J. C., and Blaustein, J. D. (1995). A subgroup of LHRH neurons in guinea pigs with progestin receptors is centrally positioned within the total population of LHRH neurons.Neuroendocrinology 61: 265–275.
Kiss, J., and Halász, B. (1985). Demonstration of serotonergic axons terminating on luteinizing hormone-releasing hormone neurons in the preoptic area of the rat using a combination of immunocytochemistry and high resolution autoradiography.Neuroscience 14: 69–78.
Knigge, K. M., Hoffman, G. E., Joseph, S. A., Scott, D. E., Sladek, C. D., and Sladek, J. R., Jr. (1979). Recent advances in structure and function of the endocrine hypothalamus.Hand. Hypothal. 2: 63–164.
Knobil, E. (1990). The GnRH pulse generator.Am. J. Obstet. Gynecol. 163: 1721–1727.
Kobayashi, R. M., Lu, K. H., Moore, R. Y., and Yen, S. S. C. (1978). Regional distribution of hypothalamic luteinizing hormone-releasing hormone in proestrous rats: Effects of ovariectomy and estrogen replacement.Endocrinology 102: 98–105.
Kokoris, G. J., Lam, N. Y., Ferin, M., Silverman, A. J., and Gibson, M. J. (1988). Transplanted gonadotropin-releasing hormone neurons promote pulsatile luteinizing hormone secretion in congenitally hypogonadal (hpg) male mice.Neuroendocrinology 48: 45–52.
Koller, K. J., Wolff, R. S., Warden, M. K., and Zoeller, R. T. (1987). Thyroid hormones regulate levels of thyrotropin-releasing hormone mRNA in the paraventricular nucleus.Proc. Natl. Acad. Sci. USA 84: 7329–7333.
Kordon, C., Drouva, S. V., Martínez de la Escalera, G., and Weiner, R. I. (1994). Role of classic and peptide neuromediators in the neuroendocrine regulation of luteinizing hormone and prolactin. In Knobil, E., and Neill, J. D., et al., (eds.),The Physiology of Reproduction, 2nd ed., Raven Press, New York, pp. 1621–1682.
Kozlowski, G. P., Chu, L., Hostetter, G., and Kerdelhue, B. (1980). Cellular characteristics of immunolabeled luteinizing hormone releasing hormone (LHRH) neurons.Peptides 1: 37–46.
Krieger, D. T., Perlow, M. J., Gibson, M. J., Davies, D. T., Zimmerman, E. A., Ferin, M., and Charlton, H. M. (1982). Brain grafts reverse hypogonadism of gonadotropin releasing hormone deficiency.Nature 298: 468–471.
Krsmanovic, L. Z., Stojilkovic, S. S., Merelli, F., Dufour, S. M., Virmani, M. A., and Catt, K. J. (1992). Calcium signaling and episodic secretion of gonadotropin-releasing hormone in hypothalamic neurons.Proc. Natl. Acad. Sci. USA 89: 8462–8466.
Krsmanovic, L. Z., Stojilkovic, S. S., Mertz, L. M., Tomic, M., and Catt, K. J. (1993). Expression of gonadotropin-releasing hormone receptors and autocrine regulation of neuropeptide release in immortalized hypothalamic neurons.Proc. Natl. Acad. Sci. USA 90: 3908–3912.
Kuljis, R. O., and Advis, J. P. (1989). Immunocytochemical and physiological evidence of a synapse between dopamine-and luteinizing hormone-releasing hormone-containing neurons in the ewe median eminence.Endocrinology 124: 1579–1581.
Lambert, G. M., Rubin, B. S., and Baum, M. J. (1992). Sex difference in the effect of mating of c-fos expression in luteinizing hormone-releasing hormone neurons of the ferret forebrain.Endocrinology 131: 1473–1480.
Layman, L. C., Wilson, J. T., Huey, L. O., Lanclos, K. D., Plouffe, L., Jr., and McDonough, P. G. (1992). Gonadotropin-releasing hormone, follicle-stimulating hormone beta, luteinizing hormone beta gene structure in idiopathic hypogonadotropic hypogonadism.Fertil. Steril. 57: 42–49.
Lee, W.-S., Abbud, R., Hoffman, G. E., and Smith, S. (1993). Effects of N-methyl-D-aspartate receptor activation on cFos expression in luteinizing hormone-releasing hormone neurons in female rats.Endocrinology 133: 2248–2254.
Lee, W.-S., Smith, M. S., and Hoffman, G. E. (1990a). Luteinizing hormone-releasing hormone neurons expressfos protein during the proestrous surge of luteinizing hormone.Proc. Natl. Acad. Sci. USA 87: 5163–5167.
Lee, W.-S., Smith, M. S., and Hoffman, G. E. (1990b). Progesterone enhances the surge of luteinizing hormone by increasing the activation of luteinizing hormone-releasing hormone neurons.Endocrinology 127: 2604–2606.
Lee, W.-S., Abbud, R., Smith, M. S., and Hoffman, G. F. (1992a). LHRH neurons express c-Jun protein during the proestrous surge of luteinizing hormone.Endocrinology 130: 3101–3103.
Lee, W.-S., Smith, M. S., and Hoffman, G. E. (1992b). C-fos activity identifies recruitment of LHRH neurons during the ascending phase of the proestrous luteinizing hormone surge.J. Neuroendocrinol. 4: 161–166.
Legan, S. J., Coon, G. A., and Karsh, F. J. (1975). Role of estrogen as initiator of daily LH surges in the ovariectomized rat.Endocrinology 96: 50–56.
Lehman, M. N., and Karsch, F. J. (1993). Do gonadotropin-releasing hormone, tyrosine hydroxylase-, and beta-endorphin-immunoreactive neurons contain estrogen receptors? A double-label immunocytochemical study in the Suffolk ewe.Endocrinology 133: 887–895.
Leonardelli, J., and Tramu, G. (1979). Immunoreactivity for β-endorphin in LHRH neurons of the fetal human hypothalamus.Cell Tissue Res. 203: 201–207.
Léránth, C., MacLusky, N. J., Sakamoto, H., Shanabrough, M., and Naftolin, F. (1985a). Glutamic acid decarboxylase containing axons synapse on LHRH neurons in the rat medial preoptic area.Neuroendocrinology 40: 536–539.
Léránth, C., Seguraum, L. M. G., Palkovits, M., MacLusky, J., Shanabrough, M., and Naftolin, F. (1985b). The LH-RH-containing neuronal network in the preoptic area of the rat: Demonstration of LH-RH-containing nerve terminals in synaptic contact with LH-RH neurons.Brain Res. 345: 332–336.
Léránth, C., MacLusky, N. J., Shanabrough, M., and Naftolin, F. (1988a). Catecholaminergic innervation of luteinizing hormone-releasing hormone and glutamic acid decarboxylase immunopositive neurons in the rat medial preoptic area.Neuroendocrinology 48: 591–602.
Léránth, C., MacLusky, N. J., Shanabrough, M., and Naftolin, F. (1988b). Immunohistochemical evidence for synaptic connections between pro-opiomelanocortin-immunoreactive axons and LH-RH neurons in the preoptic area of the rat.Brain Res. 449: 167–176.
Léránth, C., MacLusky, N. J., Brown, T. J., Chen, E. C., Redmond, D. E. J., and Naftolin, F. (1992). Transmitter content and afferent connections of estrogen-sensitive progestin receptor-containing neurons in the primate hypothalamus.Neuroendocrinology 55: 667–682.
Levine, J. E., and Duffy, M. T. (1988). Simultaneous measurement of luteinizing hormone (LH)-releasing hormone, LH, and follicle-stimulating hormone release in intact and short-term castrate rats.Endocrinology 122: 2211–2221.
Li, S., and Pelletier, G. (1992). Role of dopamine in the regulation of gonadotropin-releasing hormone in the male rat brain as studied byin situ hybridization.Endocrinology 131: 395–399.
Li, S., and Pelletier, G. (1993a). Chronic administration of muscimol and pentobarbital decreases gonadotropin-releasing hormone mRNA levels in the male rat hypothalamus determined by quantitativein situ hybridization.Neuroendocrinology 58: 136–139.
Li, S., and Pelletier, G. (1993b). Opioid regulation of gonadotropin-releasing hormone gene expression in the male rat brain as studied byin situ hybridization.Neuroreport 4: 331–333.
Liaw, J.-J., and Barraclough, C. A. (1993).N-Methyl-D,L-aspartic acid differentially affects LH release and LHRH mRNA levels in estrogen-treated ovariectomized control and androgen-sterilized rats.Mol. Brain Res. 17: 112–118.
Lightman, S. L., and Young, W. S., III (1987). Vasopressin, oxytocin, dynorphin, enkephalin, and corticotrophin releasing factor mRNA stimulation in the rat.J. Physiol. 394: 23–39.
Liposits, Z., Sétáló, G., and Flerkó, B. (1984). Application of the silver-gold intensified 3,3′-diaminobenzidine chromagen to the light and electron microscopic detection of the luteinizing hormone releasing hormone system of the rat brain.Neuroscience 13: 513–525.
Liposits, Z., Merchenthaler, I., Wetsel, W. C., Reid, J. J., Mellon, P. L., Weiner, R. I., and Negro-Vilar, A. (1991). Morphological characterization of immortalized hypothalamic neurons synthesizing luteinizing hormone-releasing hormone.Endocrinology 129: 1575–1583.
Livne, I., Gibson, M. J., and Silverman, A. J. (1993). Gonadotropin-releasing hormone (GnRH) neurons in the hypogonadal mouse elaborate normal projections despite their biosynthetic deficiency.Neuroscience 151: 229–233.
MacLusky, N. J., Naftolin, F., and Léránth, C. (1988). Immunocytochemical evidence for direct synaptic connections between corticotrophin-releasing factor (CRF) and gonadotrophin-releasing hormone (GnRH)-containing neurons in the preoptic area of the rat.Brain Res. 439: 391–395.
Maggi, R., Pimpinelli, F., Martini, L., and Piva, F. (1995). Inhibition of luteinizing hormone-releasing hormone secretion by delta-opiod agonists in GT1-1 neuronal cells.Endocrinology 136: 5177–5181.
Mahachoklertwattana, P., Sanchez, J., Kaplan, S. L., and Grumbach, M. M. (1994). N-Methyl-d-aspartate (NMDA) receptors mediate the release of gonadotropin-releasing hormone (GnRH) by NMDA in a hypothalamic GnRH neuronal cell line (GT1-1).Endocrinology 134: 1023–1030.
Majewska, M. D. (1992). Neurosteroids: endogenous bimodal modulators of the GABAA receptor. Mechanism of action and physiological significance.Prog. Neurobiol. 38: 379–395.
Majzoub, J. A., Rich, A., VanBoom, J., and Haberner, J. F. (1983). Vasopressin and oxytocin mRNA regulation in cleotides.J. Biol. Chem. 258: 14061–14064.
Malik, K. F., Silverman, A.-J., and Morrell J. I. (1991). Gonadotropin-releasing hormone mRNA in the rat: Distribution and neuronal content over the estrous cycle and after castration of males.Anat. Rec. 231: 457–466.
Marks, D. L., Smith, M. S., Vrontakis, M., Clifton, D. K., and Steiner, R. A. (1993). Regulation of galanin gene expression in gonadotropin-releasing hormone neurons during the estrous cycle of the rat.Endocrinology 132: 1836–1844.
Marks, D. L., Lent, K. L., Rossmanith, W.G., Clifton, D. K., and Steiner, R. A. (1994). Activation-dependent regulation of galanin gene expression in gonadotropin-releasing hormone neurons in the female rat.Endocrinology 134: 1991–1998.
Marshall, P. E., and Goldsmith, P. C. (1980). Neuroregulatory and neuroendocrine GnRH pathways in the hypothalamus and forebrain of the baboon.Brain Res. 193: 353–372.
Martínez, D. T., Dupont, E., Simard, J. Couet, J., Labrie, C., Labrie, F., and Pelletier, G. (1989). Regulation of luteinizing hormone-releasing hormone (LHRH) mRNA cellular levels by sex steroids in the rat brain as studied byin situ hybridization.J. Histochem. Cytochem. Abstr. 37: 934.
Martínez de la Escalera, G., Choi, A. L. H., and Weiner, R. I. (1992a). α1 regulation of the GT1 gonadotropin-releasing hormone (GnRH) neuronal cell lines: Stimulation of GnRH release via receptors positively coupled to adenylate cyclase.Endocrinology 131: 1397–1402.
Martínez de la Escalera, G., Choi, A. L. H., and Weiner, R. I. (1992b). Generation and synchronization of gonadotropin-releasing hormone (GnRH) pulses: Intrinsic properties of the GT1-1 GnRH neuronal cell line.Proc. Natl. Acad. Sci. USA 89: 1852–1855.
Martínez de la Escalera, G., Gallo, F., Choi, A. L. H., and Weiner, R. I. (1992c). Dopaminergic regulation of the GT1 gonadotropin-releasing hormone (GnRH) neuronal cell lines: Stimulation of GnRH release via D1-receptors positively coupled to adenylate cyclase.Endocrinology 131: 2965–2971.
Mason, A. J., Hayflick, J. S., Zoeller, R. T., Young, W. S., III, Phillips, H. S., Nikolics, K., and Seeburg, P. H. (1986a). A deletion truncating the GnRH gene is responsible for hypogonadism in thehpg mouse.Science 234: 1366–1371.
Mason, A. J., Pitts, S. L., Nikolics, K., Szónyi, E., Wilcox, J. N., Seeburg, P. H., and Stewart, T. A. (1986b). The hypogonadal mouse: Reproductive functions restored by gene therapy.Science 234: 1372–1378.
Matsuo, H., Baba, Y., Nau, R. M. G., Arimura, A., and Schally, A. V. (1971). Structure of the porcine LH and FSH releasing hormone. I. Proposed amino acid sequence.Biochem. Biophys. Res. Commun. 43: 1334–1339.
McCann, S. M. (1962). A hypothalamic luteinizing-hormone releasing factor.Am. J. Physiol. 202: 395–400.
McCann, S. M., Taleisnik, S., and Friedman, H. M. (1960). LH-releasing activity in hypothalamic extracts.Proc. Soc. Exp. Biol. Med. 104: 432–434.
McCarthy, M. M., Chung, S. K., Ogawa, S., Kow, L.-M., and Pfaff, D. W. (1991a). Behavioral effects of oxytocin: Is there a unifying principal? In Hard, S., and Jamison, R. (eds.).Vasopressin, John Libbey Eurotext Ltd., Paris, Vol 28, pp. 195–212.
McCarthy, M. M., Pfaff, D. W., and Schwartz-Giblin, S. (1991b). Midbrain central gray GABAA receptor activation enhances, and blockade reduces, sexual behavior in the female rat.Exp. Brain Res. 86: 108–116.
McEwen, B. S. (1991). Non-genomic and genomic effects of steroids on neural activity.Trends Pharmacol. Sci. 12: 141–147.
Mellon, P. L., Windle, J. J., Goldsmith, P. C., Padula, C. A., Roberts, J. L., and Weiner, R. I. (1990). Immortalization of hypothalamic GnRH neurons by genetically targeted tumorigenesis.Neuron 5: 1–10.
Mellon, P. L., Windle, J. J., and Weiner, R. I. (1991). Immortalization of neuroendocrine cells by targeted oncogenesis.Rec. Prog. Horm. Res. 47: 69–96.
Merchenthaler, I. (1991). Neurons with access to the general circulation in the central nervous system of the rat: A retrograde tracing study with fluoro-gold.Neuroscience 44: 655–662.
Merchenthaler, I., Görcs, T., Sétáló, G., Petrusz, P., and Flerkó, B. (1984). Gonadotropin-releasing hormone (GnRH) neurons and pathways in the rat brain.Cell Tissue Res. 237: 15–29.
Merchenthaler, I., Culler, M. D., Negro-Villar, A., Petrusz, P. and Flerkó, B. (1988). The proLHRH system of the rat brain. Effects of changes in the endocrine background.Brain Res. Bull. 20: 713–720.
Merchenthaler, I., López, F., and Negro-Vilar, A. (1990). Colocalization of galanin and luteinizing hormone-releasing hormone in a subset of preoptic hypothalamic neurons: Anatomical and functional correlates.Proc. Natl. Acad. Sci. USA 87: 6326–6330.
Merchenthaler, I., Sétáló, G., Csontos, C., Petrusz, P., Flerkó, B., and Negro-Villar, A. (1989a). Combined retrograde tracing and immunocytochemical identification of luteinizing hormone-releasing hormone-and somatostatin-containing neurons projecting to the median eminence of the rat.Endocrinology 125: 2812–2821.
Merchenthaler, I., Sétáló, G., Petrusz, P., Negro-Vilar, A., and Flerkó, B. (1989b). Identification of hypophysiotropic luteinizing hormone-releasing hormone (LHRH) neurons by combined retrograde labeling and immunocytochemistry.Exp. Clin. Endocrinol. 94: 133–140.
Meredith, J. M., and Levine, J. E. (1992). Effects of castration on LH-RH patterns in intrahypophysial microdialysates.Brain Res. 571: 181–188.
Milenković, L., D'Angelo, G., Kelly, P. A., and Weiner, R. I. (1994). Inhibition of gonadotropin hormone-releasing hormone release by prolactin from GT1 neuronal cell lines through prolactin receptors.Proc. Natl. Acad. Sci. USA 91: 1244–1247.
Millar, R. P., Wormald, P. J., and Milton, R. C. de L. (1986). Stimulation of gonadotropin release by a non-GnRH peptide sequence of the GnRH precursor.Science 232: 68–70.
Milton, R. C. de L., Wormald, P. J., Brandt, W., and Millar, R. P. (1986). The delineation of a decapeptide gonadotropin-releasing sequence in the carboxyl-terminal extension of the human gonadotropin-releasing hormone precursor.J. Biol. Chem. 261: 16990–16997.
Moenter, S. M., Karsch, F. J., and Lehman, M. N. (1993).Fos expression during the estradiol-induced gonadotropin-releasing hormone (GnRH) surge of the ewe: Induction in GnRH and other neurons.Endocrinology 133: 896–903.
Mora, S., Afani, A., Kusanovic, R., Tapia, C., and Diaz-Veliz, G. (1991). Behavioral effects of intracerebral administration of luteinizing hormone releasing hormone (LHRH) in rats.Pharmacol. Biochem. Behav. 3: 705–709.
Moss, R. L., and Dudley, C. A. (1990). Differential effects of a luteinizing hormone-releasing hormone (LHRH) antagonist analogue on lordosis behavior induced by LHRH and the LHRH fragment Ac-LHRH3–10.Neuroendocrinology 52: 138–142.
Moss, R. L., and Foreman, M. M. (1976). Potentiation of lordosis behavior by intrahypothalamic infusion of synthetic luteinizing hormone-releasing hormone.Neuroendocrinology 20: 176–181.
Moss, R. L., and McCann, S. M. (1973). Induction of mating behavior in rats by luteinizing hormone-releasing factor.Science 181: 177–179.
Mrowiec, J., Plech, A., Rzeszotarska, B., Ryszka F., Brus, R., and Masiukiewicz, E. (1992). Behavioral effects of luteinizing hormone-releasing hormone (LHRH) in rats.J. Physiol. Pharmacol. 43: 271–278.
Myers, B. M., and Baum, M. J. (1980). Facilitation of copulatory performance in male rats by naloxone: Effects of hypophysectomy, 17α-estradiol, and luteinizing hormone releasing hormone.Pharmacol. Biochem. Behav. 12: 365–370.
Naftolin, F., Brown-Grant, K., and Corker, C. S. (1972). Plasma and pituitary luteinizing hormone and peripheral plasma oestradiol concentrations in the normal oestrus cycle of the rat and after, experimental manipulation of the cycle.J. Endocrinol. 53: 17–30.
Nakai, Y., Ochiai, H., Kitazawa, S., Shioda, S., and Wantanabe, T. (1985). Electron microscopic cytochemistry of the relationship between catecholaminergic axons and peptidergic neurons in the rat hypothalamus. In Kobayashi, H., Bern, H. A., and Urano, A. (eds.).Neurosecretion and the Biology of Neuropeptides. Jpn. Sci. Soc. Press, Tokyo, pp. 121–129.
Nakayama, Y., Wondisford, F. E., Lash, R. W., Bale, A. E., Weintraub, B. D., Cutler, G. B., Jr and Radovick, S. (1990). Analysis of gonadotropin-releasing hormone gene structure in families with familial central precocious puberty and idiopathic hypogonadotropic hypogonadism.J. Clin. Endocrinol. Metab. 70: 1233–1238.
Neill, J. D., Patton, J. M., Dailey, R. A., Tsou, R. C., and Tindall, G. T. (1977). Luteinizing hormone releasing hormone (LHRH) in pituitary stalk blood of rhesus monkeys: Relationship to level of LH release.Endocrinology 101: 430–434.
Nikolics, K., Mason, A. J., Szónyi, E., Ramachandran, J., and Seeburg, P. H. (1985). A prolactin-inhibiting factor within the precursor for human gonadotropin-releasing hormone.Nature 316: 511–517.
Noris, G., Hol, D., Clapp, C., and Martínez de la Escalera G. (1995). Histamine directly stimulates gonadotropin-releasing hormone secretion from GT1–1 cells via H1 receptors coupled to phosphoinositide hydrolysis.Endocrinology 136: 2967–2974.
Ogawa, S., Kow, L.-M., and Pfaff, D. W. (1992). Effects of lordosis-relevant neuropeptides on midbrain periaqueductal gray neuronal activityin vitro.Peptides 13: 965–975.
Palkovits, M., Brownstein, M., Schally, A. V., and Saavedra, J. M. (1974). Luteinizing hormone-releasing hormone (LH-RH) content of the hypothalamic nuclei in rat.Endocrinology 95: 554–558.
Palkovits, M., Mezey, E., Ambach, G. and Kivovics, P. (1978). Neural and vascular connections between the organum vasculosum laminae terminalis and preoptic nuclei. In Scott, D. E., Kozlowski, G. P. and Weindl, A. (eds.).Brain-Endocrine Interaction III. Neural Hormones and Reproduction. Karger, Basel, pp. 302–312.
Palkovits, M., Léránth, Cs., Jew, J. Y., and Williams, T. (1982). Simultaneous characterization of pre-and postsynaptic neuron contact sites in brain.Proc. Natl. Acad. Sci. USA 79: 2705–2708.
Pan, J.-T., Kow, L.-M., and Pfaff, D. W. (1988). Modulatory actions of luteinizing hormone-releasing hormone on electrical activity of preoptic neurons in brain slices.Neuroscience 27: 623–628.
Park, Y., Park, S. D., Cho, W. K., and Kim, K. (1988). Testosterone stimulates LH-RH-like mRNA level in the rat hypothalamus.Brain Res. 451: 255–260.
Park, O.-K., Gugneja, S., and Mayo, K. E., (1990). Gonadotropin-releasing hormone gene expression during the rat estrous cycle: Effects of pentobarbital and ovarian steroids.Endocrinology 127: 365–372.
Pellegrino, L. J., Pellegrino, A. S., and Cushman, A. J. (1979).A Stereotaxic Atlas of the Rat Brain, 2nd ed., Plenum Press, New York.
Petersen, S. L., Cheuk, C., Hartman, R. D., and Barraclough, C. A. (1989). Medial preoptic microimplants of the antiestrogen, keoxifene, affect luteinizing hormone-releasing hormone mRNA levels, median eminence luteinizing hormone-releasing hormone concentrations and luteinizing hormone release in ovariectomized estrogen-treated rats.J. Neuroendocrinol. 1: 279–283.
Petersen, S. L., McCrone, S., Keller, M., and Gardner, E. (1991). Rapid increase in LHRH mRNA levels following NMDA.Endocrinology 129: 1679–1681.
Petersen, S. L., McCrone, S., Coy, D., Adelman, J. P., and Mahan, L. C. (1993a). GABAA receptor subunit mRNAs in cells of the preoptic area: Colocalization with LHRH mRNA using dual-labelin situ hybridization histochemistry.Endocrine J. 1: 29–34.
Petersen, S. L., McCrone, S., and Shores, S. (1993b). Localized changes in LHRH mRNA levels as cellular correlates of the positive feedback effects of estrogen on LHRH neurons.Am. Zool. 33: 255–265.
Petersen, S. L., McCrone, S., Keller, M., and Shores, S. (1995). Effects of estrogen and progesterone on luteinizing hormone-releasing hormone messenger ribonucleic acid levels: Consideration of temporal and neuroanatomical variables.Endocrinology 136: 3604–3610.
Pfaff, D. W. (1986). Gene expression in hypothalamic neurons: luteinizing hormone-releasing hormone.J. Neurosci. Res. 16: 109–115.
Pfaff, D. W. (1973). Luteinizing hormone-releasing factor potentiates lordosis behavior in hypophysectomized ovariectomized female rats.Science 182: 1148–1149.
Pfaff, D. W., and Keiner, M. (1973). Atlas of estradiol-concentrating cells in the central nervous system of the female rat.J. Comp. Neurol. 151: 121–158.
Pfaff, D. W., Schwanzel-Fukuda, M., Parhar I. S., Lauber, A. H., McCarthy, M. M., and Kow L.-M. (1994a). GnRH neurons and other cellular and molecular mechanisms for simple mammalian reproductive behaviors.Rec. Prog. Horm. Res. 49: 1–25.
Pfaff, D. W., Schwartz-Giblin, S., McCarthy, M. M., and Kow, L.-M. (1994b). Cellular mechanisms of female reproductive behaviors. In Knobil, E., and Neill, J. D.et al., (eds.),The Physiology of Reproduction, 2nd ed., Raven Press, New York, pp. 107–220.
Pfaus, J. G., Jakob, A., Kleopoulos, S. P., Gibbs, R. B., and Pfaff, D. W. (1994). Sexual stimulation induces Fos immunoreactivity within GnRH neurons of the female rat preoptic area: Interaction with steroid hormones.Neuroendocrinology 60: 283–290.
Phelps, C. P., Kalra, S. P., and Kalra, P. S. (1992).In vivo pulsatile GnRH release into the anterior pituitary of the male rat: Effects of castration.Brain Res. 569: 159–163.
Phillips, H. S., Nikolics, K., Branton, D., and Seeburg, P. H. (1985). Immunocytochemical localization in rat brain of a prolactin release-inhibiting sequence of gonadotropinreleasing hormone prohormone.Nature 316: 542–545.
Poletti, A., Melcangi, R. C., Negri-Cesi, P., Maggi, R., and Martini, L. (1994). Steroid binding and metabolism in the luteinizing hormone-releasing hormone-producing neuronal cell line GT1-1.Endocrinology 135: 2623–2628.
Porkka-Heiskanen, T., Urban, J. H., Turek, F. W. and Levine, J. E. (1994). Gene expression in a subpopulation of luteinizing hormone-releasing hormone (LHRH) neurons prior to the preovulatory gonadotropin surge.J. Neurosci. 14: 5548–5558.
Price, D. (1975). Feedback control of gonadal and hypophyseal hormones: Evolution of the concept. In Meites, J., Donovan, B. T., and McCann, S. M. (eds.).Pioneers in Neuroendocrinology, Plenum Press, New York, pp. 219–238.
Pycock, C. J., and Taberner, P. V. (eds.) (1981).Central Neurotransmitter Turnover, University Park Press, Baltimore.
Radovick, S., Wondisford, F. E., Nakayama, Y., Yamada, M., Cutler, G. B. Jr., and Weintraub, B. D. (1990). Isolation and characterization of the human gonadotropin-releasing hormone gene in the hypothalamus and placenta.Mol. Endocrinol. 4: 476–480.
Radovick, S., Ticknor, C. M., Nakayama, Y., Notides, A. C., Rahman, A., Weintraub, B. D., Cutler, G. B., Jr., and Wondisford, F. E. (1991a). Evidence for direct estrogen regulation of the human gonadotropin-releasing hormone gene.J. Clin. Invest. 88: 1649–1655.
Radovick, S., Wray, S., Lee, E., Nichols, D. K., Nakayama, Y., Weintraub, B. D., Westphal, H., Cutler, G. B., Jr., and Wondisford, F. E. (199b). Migratory arrest of gonadotropin-releasing hormone neurons in transgenic mice.Proc. Natl. Acad. Sci. USA 88: 3402–3406.
Rajendren, G., and Moss, R. L. (1993). The role of the medial nucleus of amygdala in the mating-induced enhancement of lordosis in female rats: The interaction with luteinizing hormone-releasing hormone neuronal system.Brain Res. 617: 81–86.
Ramirez, V. D., and Dluzen, D. E. (1987). Release of luteinizing hormone-releasing hormone (LHRH) and neuroactive substances in unanesthetized animals as estimated with push-pull cannulae (PPC).Biol. Reprod. 36: 59–76.
Ramirez, V. D., Dluzen, D., and Lin, D. (1980). Progesterone administrationin vivo stimulates release of luteinizing hormone releasing hormonein vitro.Science 208: 1037–1039.
Ramirez, V. D., Feder, H. H., and Sawyer, C. H. (1984). The role of brain catecholamines in the regulation of LH secretion: A critical inquiry.Front. Neuroendocrinol. 8: 27–84.
Rance, N., Wise, P. M., and Barraclough, C. A. (1981). Negative-feedback effects of progesterone correlated with changes in hypothalamic norepinephrine and dopamine turnover rates, median eminence luteinizing hormone-releasing hormone, and peripheral plasma gonadotropins.Endocrinology 108: 2194–2199.
Reinhart, J., Mertz, I. M., and Catt, K. J. (1992). Molecular cloning and expression of cDNA encoding the murine gonadotropin-releasing267: 21281–21284.
Reubi, J. C., and Maurer, R. (1985). Visualization of LHRH receptors in the rat brain.Eur. J. Pharmacol. 106: 453–454.
Riskind, P., and Moss, R. L. (1983). Midbrain LHRH infusions enhance lordotic behavior in ovariectomized estrogenprimed rats independently of hypothalamic responsiveness to LHRH.Brain Res. Bull. 11: 481–485.
Roberts, J. L., Dutlow, C. M., Jakubowski, M., Blum, M., and Miller, R. P. (1989). Estradiol stimulates preoptic areaanterior hypothalamic proGnRH-GAP gene expression in ovariectomized rats.Mol. Brain Res. 6: 127–134.
Rodriguez, E. M. (1976). The cerebrospinal fluid as a pathway in neuroendocrine integration.J. Endocrinol.,71: 407–443.
Ronnekleiv, O. K., Adelman, J. P., Weber, E., Herbert, E., and Kelly, M. J. (1987). Immunohistochemical demonstration of proGnRH and GnRH in the preoptic-basal hypothalamus of the primate.Neuroendocrinology 45: 518–521.
Ronnekleiv, O. K., Naylor, B. R., Bond, C. T., and Adelman, J. P. (1989). Combined immunocytochemistry for gonadotropin-releasing hormone (GnRH) and pro-GnRH, andin situ hybridization for GnRH messenger ries with familiain rat brain.Mol Endocrinol. 3: 363–371.
Roselli, C. E., Kelly, M. J., and Ronnekleiv, O. K. (1990). Testosterone regulates progonadotropin-releasing hormone levels in preoptic area and basal hypothalamus of the male rat.Endocrinology 126: 1080–1086.
Rosie, R., Thomson, E., and Fink, G. (1990). Oestrogen positive feedback stimulates the synthesis of LHRH mRNA in neurones of the rostral diencephalon of the rat.J. Endocrinol. 124: 285–289.
Rothfeld, J. M., Hejtmancik, J. F., Conn, P. M., and Pfaff, D. W. (1987). LHRH messenger RNA in neurons in the intact and castrate male rat forebrain, studied byin situ hybridization.Exp. Brain Res. 67: 113–118.
Rothfeld, J. M., Hejtmancik, J. F., Conn, P. M., and Pfaff, D. W. (1989).In situ hybridization for LHRH mRNA following estrogen treatment.Mol. Brain Res. 6: 121–125.
Rubin, B. S., King, J. C., Millar, R. P., Seeburg, P. H., and Arimura, A. (1987). Processing of luteinizing hormonereleasing hormone precursor in rat neurons.Endocrinology 121: 305–309.
Sakuma, Y., and Pfaff, D. W. (1980). LH-RH in the mesencephalic central grey can potentiate lordosis reflex of female rats.Nature 283: 566–567.
Sakuma, Y., and Pfaff, D. W. (1983). Modulation of the lordosis reflex of female rats by LHRH, its antiserum and analogs in the mesencephalic central gray.Neuroendocrinology 36: 218–224.
Schally, A. V., Arimura, A., Baker, Y., Nair, R. M. G., Matsuo, H., Redding, T. W., Debeljuk, L., and White, W. F. (1971). Isolation and properties of the FSH-and LH-releasing hormone.Biochem. Biophys. Res. Commun. 43: 393–399.
Schumacher, M. (1990). Rapid membrane effects of steroid hormones: An emerging concept in neuroendocrinology.Trends Neurosci. 13: 359–362.
Schwanzel-Fukuda, M., and Silverman, A. J. (1980). The nervus terminalis of the guinea pig: A new luteinizing hormone releasing hormone neuronal system.J. Comp. Neurol. 191: 213–224.
Schwanzel-Fukuda, M., Bick, D., and Pfaff, D. W. (1989). Luteinizing hormone-releasing hormone (LHRH)-expressing cells do not migrate normally in an inherited hypogonadal (Kallmann) syndrome.Mol. Brain Res. 6: 311–326.
Schwanzel-Fukuda, M., Jorgenson, K. L., Bergen, H. T., Weesner, G. D., and Pfaff, D. W. (1992a). Biology of normal luteinizing hormone-releasing hormone neurons during and after their migration from olfactory placode.Endocrine Rev. 13: 623–634.
Schwanzel-Fukuda, M., Zheng, L. M., Bergen, H. T., Weesner, G. D., and Pfaff, D. W. (1992b). LHRH neurons: Functions and development.Prog. Brain Res. 93: 189–203.
Seeburg, P. H., and Adelman, J. P. (1984). Characterization of cDNA for precursor of human luteinizing hormone releasing hormone.Nature 311: 666–668.
Seeburg, P. H., Mason, A. J., Stewart, T. A., and Nikolics, K. (1987). The mammalian GnRH gene and its pivotal role in reproduction.Rec. Prog. Horm. Res. 43: 69–98.
Seeburg, P. H., Mason, A. J., Young, W. S. III, Stewart, T. A., and Nikolics, K. (1989). The gene encoding GnRH and its associated peptide GAP: Some insights into hypogonadism.J. Steroid Biochem. 33: 687–691.
Selmanoff, M. (1981). The lateral and medial median eminence: Distribution of dopamine, norepinephrine, and luteinizing hormone-releasing hormone and the effect of prolactin on catecholamine turnover.Endocrinology 108: 1716–1722.
Selmanoff, M. K., Wise, P. M., and Barraclough, C. A. (1980). Regional distribution of luteinizing hormone-releasing hormone (LH-RH) in rat brain determined by microdissection and radioimmunoassay.Brain Res. 192: 421–432.
Selmanoff, M., Shu, C., Petersen, S. L., Barraclough, C. A., and Zoeller, R. T. (1991). Single cell levels of hypothalamic messenger ribonucleic acid encoding luteinizing hormone-releasing hormone in intact, castrated, and hyperprolactinemic male rats.Endocrinology 128: 459–466.
Seong, J. Y., Lee, K. Y., Lee, C. C., and Kim, K. (1993). NMDA receptor antagonist decreases the progesterone-induced increase in GnRH gene expression in the rat hypothalamus.Neuroendocrinology 58: 234–239.
Sétáló, G., Vigh, S., Arimura, A., and Schally, A. V. (1978). Brain cells as producers of releasing and inhibiting hormones.Intl. Rev. Cytol. Suppl. 7: 1–52.
Sherwood, N. M., and Fink, G. (1980). Effect, of ovariectomy and adrenalectomy on luteinizing hormone-releasing hormone in pituitary stalk blood from female rats.Endocrinology 106: 363–367.
Shivers, B. D., Harlan, R. E., Morrell, J. I., and Pfaff, D. W. (1983a). Absence of oestradiol concentration in cell nuclei of LHRH immunoreactive neurones.Nature 304: 345–347.
Shivers, B. D., Harlan, R. E., Morrell, J. I., and Pfaff, D. W. (1983b). Immunocytochemical localization of luteinizing hormone-releasing hormone in male and female rat brains. Quantitative studies on the effect of gonadal steroids.Neuroendocrinology 36: 1–12.
Silverman, A. J., and Desnoyers, P. (1976). Ultrastructural immunocytochemical localization of LHRH in the median eminence of the guinea pig.Cell Tissue Res. 169: 157–166.
Silverman, A. J., Krey, L. C., and Zimmerman, E. A. (1979). A comparative study of luteinizing hormone-releasing hormone (LH-RH) neuronal networks in mammals.Biol. Reprod. 20: 98–110.
Silverman, A. J., Zimmerman, E. A., Gibson, J. J., Perlow, M. J., Charlton, H. M., and Krieger, D. T. (1985). Implantation of normal fetal preoptic area into hypogonadal mutant mice: Temporal relationships of the growth of gonadotropin-releasing hormone neurons and the development of the pituitary/testicular axis.Neuroscience 16: 69–84.
Silverman, A. J., Jhamandas, J., and Renaud, L. P. (1987a). Localization of luteinizing hormone-releasing hormone (LHRH) neurons that project to the median eminence.J. Neurosci. 7: 2312–2319.
Silverman, A. J., Silverman, R., Lehman, M. N., Witkin, J. W., and Millar, R. P. (1987b). Localization of a peptide sequence contained in the precursor to gonadotropin releasing hormone (GnRH).Brain. Res. 402: 346–350.
Silverman, A. J., Kokoris, G. J., and Gibson, M. J. (1988). Quantitative analysis of synaptic input to gonadotropin-releasing hormone neurons in normal mice andhpg mice with preoptic area grafts.Brain Res. 443: 367–372.
Silverman, R. C., Silverman, A. J., and Gibson, M. J. (1989). Identification of gonadotropin releasing hormone (GnRH) neurons projecting to the median eminence from third ventricular preoptic area grafts in hypogonadal mice.Brain Res. 501: 260–268.
Silverman, A. J., Roberts, J. L., Dong, K.-W., Miller, G. M., and Gibson, M. J. (1992). Intrahypothalamic injection of a cell line secreting gonadotropin-releasing hormone results in cellular differentiation and reversal of hypogonadism in mutant mice.Proc. Natl. Acad. Sci. 89: 10668–10672.
Silverman, A. J., Livne, I., and Witkin, J. W. (1994). The gonadotropin-releasing hormone (GnRH) neuronal systems: Immunocytochemistry andin situ hybridization. In Knobil, E., Neill, J. D.,et al. (eds.)The Physiology of Reproduction, 2nd ed., Raven Press New York, pp. 1683–1710.
Sirinathsinghji, D. J. S. (1983). GnRH in the spinal subarachnoid space potentiates lordosis behavior in the female rat.Physiol. Behav. 31: 717–723.
Sirinathsinghji, D. J. S. (1986). Regulation of lordosis behaviour in the female rat by corticotropin-releasing factor, β-endorphin/corticotropin and luteinizing hormone-releasing hormone neuronal systems in the medial preoptic area.Brain Res. 375: 49–56.
Sirinathsinghji, D. J. S. Whittington, P. E., and Audsley, A. R. (1986). Regulation of mating behaviour in the female rat by gonadotropin-releasing hormone in the ventral tegmental area: Effects of selective destruction of the A10 dopamine neurons.Brain Res. 374: 167–173.
Skinner, D. C., Malpaux, B., Delaleu, B., and Caraty, A. (1995). Luteinizing hormone (LH)-releasing hormone in third ventricular cerebrospinal fluid of the ewe: Correlation with LH pulses and the LH surge.Endocrinology 136: 3230–3237.
Song, T., Nikolics, K., Seeburg, P. H., and Goldsmith, P. C. (1987). GnRH-prohormone-containing neurons in the primate brain: Immunostaining for the GnRH-associated peptide.Peptides 8: 335–346.
Spratt, D. I., Finkelstein, J. S., O'Dea, L. St. L., Badger, T. M., Rao, P. N., Campbell, J. D., and Crowley, W. F. Jr. (1986). Long-term administration of gonadotropin-releasing hormone in men with idiopathic hypogonadotropic hypogonadism.Ann. Intern. Med. 105: 848–855.
Stelzer, A. (1992). GABA, receptors control the excitability of neuronal populations.Int. Rev. Neurobiol.,33: 195–287.
Sternberger, L. A., and Hoffman, G. E. (1978). Immunocytology of luteinizing hormone-releasing hormone.Neuroendocrinology 25: 111–128.
Stopa, E. G., Uhl, G. R., O'Hara, B. F., Chorsky, R. L., King, J. C., Bird, E. D., and Wolfe, H. (1992). Somatostatingene expression in the postmortem adult and fetal human brain.Peptide Res. 5: 201–205.
Stumpf, W. E., and Sar, M. (1975). Hormone-architecture of the mouse brain with3H-estradiol. In Stumpf, W. E., and Grant, L. D. (eds.),Anatomical Neuroendocrinology, Karger, Basel, pp. 82–103.
Sullivan, K. A., Witkin, J. W., Ferin, M., and Silverman, A.-J. (1995). Gonadotropin-releasing hormone neurons in the rhesus macaque are not immunoreactive for the estrogen receptor.Brain Res. 685: 198–200.
Szekely, A. M., Costa, E., and Grayson, D. R. (1990). Transcriptional program coordination by N-methyl-d-aspartate sensitive glutamate receptor stimulation in primary cultures of cerebellar neurons.Mol. Pharmacol. 38: 624–633.
Tan, L., and Rousseau, P. (1982). The chemical identity of the immunoreactive LHRH-like peptide biosynthesized in the human placenta.Biochem. Biophys. Res. Commun. 109: 1061–1071.
Tennent, B. J., Smith, E. R., and Dorsa, D. M. (1982). Comparison of some CNS effects of luteinizing hormone-releasing hormone and progesterone.Horm. Behav. 16: 76–86.
Thind, K. K., and Goldsmith, P. C. (1988). Infundibular gonadotropin-releasing hormone neurons are inhibited by direct opioid and autoregulatory synapses in juvenile monkeys.Neuroendocrinology 47: 203–216.
Thind, K. K., and Goldsmith, P. C. (1995). Glutamate and GABAergic neurointeractions in the monkey hypothalamus: A quantitative immunomorphological study.Neuroendocrinology 61: 471–485.
Thompson, T. L., and Moss, R. L. (1992). Specific binding of125I-LHRH agonist to hippocampal membranes: Fluctuations during the estrous cycle.Peptides 13:891–896.
Tischkau, S. A., and Ramirez, V. D. (1993). A specific membrane binding protein for progesterone in rat brain: Sex differences and induction by estrogen.Proc. Natl. Acad. Sci. USA 90:1285–1289.
Toranzo, D., Dupont, E., Simard, J., Labrie, C., Couet, J., Labrie, F., and Pelletier, G. (1989). Regulation of pro-gonadotropin releasing hormone gene expression by sex steroids in the brain of male and female rats.Mol. Endocrinol. 3:1748–1756.
Touchette, N. (1990). Man bites dogma: A new role for steroid hormones.J. NIH Res. 2:71–74.
Tsuruo, Y., Kawano, H., Kagotani, Y., Hisano, S., Daikoku, S., Chihara, K., Zhang, T., and Yanaihara, N. (1990). Morphological evidence for neuronal regulation of luteinizing hormone-releasing hormone-containing neurons by neuropeptide Y in the rat septo-preoptic area.Neurosci. Lett. 110:261–266.
Tsuruo, Y., Kawano, H., Hisano, S., Kagotani, Y., Daikoku, S., Zhang, T., and Yanaihara, N. (1991). Substance P-containing neurons innervating LHRH-containing neurons in the septo-preoptic area of rats.Neuroendocrinology 53: 236–245.
Tsutsumi, M., Zhou, W., Millar, R. P., Mellon, P. L., Roberts, J. L., Flanagan, C. A., Doug, K., Gillo, B., and Sealfon, S. C. (1992). Cloning and functional expression of a mouse gonadotropin-releasing hormone receptor.Mol. Endocrinol. 6:1163–1169.
Turgeon, J. L., and Barraclough, C. A. (1973). Temporal patterns of LH release following graded preoptic electrochemical stimulation in proestrous rats.Endocrinology 92:755–761.
Uhl, G. R., and Nishimori, T. (1990). Neuropeptide gene expression and neural activity: Assessing a working hypothesis in nucleus caudalis and dorsal horn neurons expressing preproenkephalin and preprodynorphin.Cell Mol. Neurobiol. 10:73–98.
Uhl, G. R., Evans, J., Parta, M., Walworth, C., Hill, K., Sasek, C., Voigt, M. and Reppert, S. (1986). Vasopressin and somatostatin mRNAin situ hybridization. In Uhl, G. R. (ed.),In Situ Hybridization in Brain, Plenum Press, New York, pp. 21–47.
Urbanski, H. F., Fahy, M. M., Daschel, M., and Mehull, C. (1994). N-Methyl-d-aspartate receptor gene expression in the hamster hypothalamus and in immortalized luteinizing hormone-releasing hormone neurones.J. Reprod. Fertil. 100:5–9.
Valenca, M. M., Ching, M., Masotto, C., and Negro-Vilar A. (1987). How does the gonad affect LHRH secretion? Effects of gonadectomy on LHRH release from median eminence nerve terminals incubatedin vitro and on LHRH concentration in hypophyseal portal blood.Adv. Exp. Med. Biol. 219:617–621.
Vallet, P. G., Charnay, Y., Bouras, C., and Kiss, J. Z. (1991). Colocalization of delta sleep-inducing peptide and luteinizing releasing hormone in neurosecretory vesicles in rat median eminence.Neuroendocrinology 53:103–106.
Van Vugt, D. A., Diefenbach, W. P., Alston, E., and Ferin, M. (1985). Gonadotropin-releasing hormone pulses in third ventricular cerebrospinal fluid of ovariectomized rhesus monkeys: Correlation with luteinizing hormone pulses.Endocrinology 117:1550–1558.
Vitalis, E. A., Martinez de la Escalera, G., Hemmings, H. C., Jr., and Weiner, R. I. (1993) Expression and regulation of the phosphorylation of DARPP in the GT1 GnRH-secreting cell line.Endocrine Soc. Abstr. 1185, p. 347.
Ward, B. J., and Charlton, H. M. (1981). Female sexual behavior in the GnRH deficient, hypogonadal (hpg) mouse.Physiol. Behav. 27:1107–1109.
Watanabe, T., and Nakai, Y. (1987). Electron microscopic cytochemistry of catecholaminergic innervation of LHRH neurons on the medial preoptic area of the rat.Arch. Histol. Jpn. 50:103–112.
Watson, R. E., Jr., Langub, M. C., Jr., and Landis, J. W. (1992). Further evidence that most LHRH neurons are not directly estrogen responsive: Simultaneous localization of LHRH and estrogen-receptor immunoreactivity in the guinea pig brain.J. Neuroendocrinol. 4:311–317.
Weesner, G. D., Bergen, H. T., and Pfaff, D. W. (1992). Differential regulation of luteinizing hormone-releasing hormone and galanin messenger ribonucleic acid levels by alpha1-adrenergic agents in the ovariectomized rat,J. Neuroendocrinol. 4:331–336.
Weesner, G. D., Krey, L. C., and Pfaff, D. W. (1993). α1 Adrenergic regulation of estrogen-induced increases in luteinizing hormone-releasing hormone mRNA level and release.Mol. Brain Res. 17:77–82.
Weindl, A. (1973). Neuroendocrine aspects of circumventricular organs. In Ganong, W. F., and Martini, L. (eds.),Frontiers in Neuroendocrinology, Oxford University Press, New York, pp. 3–32.
Weindl, A., and Sofroniew, M. V. (1978) Neurohormones and circumventricular organs. In Scott, D. E., Kozlowski, G. P., and Weindl, A. (eds.),Brain-Endocrine Interaction III, Neural Hormones and Reproduction, Karger, Basel, pp. 117–137.
Weiner, R. I., Wetsel, W., Goldsmith, P., Martinez de la Escalera, G., Windle, J., Padula, C., Choi, A., Negro-Vilar, A., and Mellon, P. (1992). Gonadotropin-releasing hormone neuronal cell lines.Front. Neuroendocrinol. 13: 95–119.
Weiss, J., Crowley, W. F., Jr., and Jarneson, J. L. (1989). Normal structure of the gonadotropin-releasing hormone (GnRH) gene in patients with GnRH deficiency and idiopathic hypogonadotropic hypogonadism.J. Clin. Endocrinol. Metab. 69:299–303.
Weiss, J., Adams, E., Whitcomb, R. W., Crowley, W. F., Jr., and Jameson, J. L. (1991). Normal sequence of the gonadotropin-releasing hormone gene in patients with idiopathic hypogonadotropic hypogonadism.Biol. Reprod. 45:743–747.
Wetsel, W. C., and Negro-Vilar, A. (1989). Testosterone selectively influences protein kinase-C-coupled secretion of proluteinizing hormone-releasing hormone-derived peptides.Endocrinology 125:538–547.
Wetsel, W. C., Mellon, P. L., Weiner, R. I., and Negro-Vilar, A. (1991). Metabolism of pro-luteinizing hormone-releasing hormone in immortalized hypothalamic neurons.Endocrinology 129:1584–1595.
Wetsel, W. C., Valenca, M. M., Merchenthaler, I., Liposits, Z., López, F. J., Weiner, R. I., Mellon, P. L., and Negro-Villar, A. (1992). Intrinsic pulsatile secretory activity of immortalized luteinizing hormone-releasing hormone-secreting neurons.Proc. Natl. Acad. Sci. USA 89:4149–4153.
Wheaton, J. E., Krulich, L., and McCann: S. M. (1975). Localization of luteinizing hormone-releasing hormone in the preoptic area and hypothalamus of the rat using radioimmunoassay.Endocrinology 97:30–38.
Whitehead, S. A., Pennington, J. M., and Carter, D. A. (1982). Dynamic changes in pituitary responses to luteinizing hormone-releasing hormone after ovariectomy in the rat.J. Endocrinol. 93:75–81.
Wiemann, J. N., Clifton, D. K., and Steiner, R. A. (1989). Pubertal changes in gonadotropin-releasing hormone and proopiomelanocortin gene expression in the brain of the male rat.Endocrinology 124:1760–1767.
Wiemann, J. N., Clifton, D. K., and Steiner, R. A. (1990). Gonadotropin-releasing hormone messenger ribonucleic acid levels are unaltered with changes in the gonadal hormone milieu of the adult male rat.Endocrinology 127: 523–532.
Williams, C. L., Thalabard, J. C., O'Byrne, K. T., Grosser, P. M., Nishihara, M., Hotchkiss, J., and Knobil, E. (1990). Duration of phasic electrical activity of the hypothalamic gonadotropin-releasing hormone pulse generator and dynamics of luteinizing hormone pulses in the rhesus monkey.Proc. Natl. Acad. Sci. USA 87:8580–8582.
Williamson, P., Lang, J., and Boyd, Y. (1991). The gonadotropin-releasing hormone (Gnrh) gene maps to mouse chromosome 14 and identifies a homologous region on human chromosome 8.Somat. Cell Mol. Genet. 17:609–615.
Wise, P. M., Rance, N., Selmanoff, M., and Barraclough, C. A. (1981). Changes in radioimmunoassayable luteinizing hormone-releasing hormone in discrete brain areas of the rat at various times on proestrus, diestrous day 1, and after phenobarbital administration.Endocrinology 108:2179–2185.
Witkin, J. W. (1989). Morphology of luteinizing hormone-releasing hormone neurons as a function of age and hormone conditions in the male rat.Neuroendocrinology 49: 344–348.
Witkin, J. W. (1990). Access of luteinizing hormone-releasing hormone neurons to the vasculature in the rat.Neuroscience 37:501–506.
Witkin, J. W. (1992). Increased synaptic input to gonadotropin-releasing hormone neurons in aged, virgin, male Sprague-Dawley rats.Neurobiol. Aging 13:681–686.
Witkin, J. A., and Silverman, A. J. (1983). Luteinizing hormone-releasing hormone (LHRH) in rat olfactory systems.J. Comp. Neurol. 218:426–432.
Witkin, J. W., and Silverman, A. J. (1985). Synaptology of LHRH neurons in rat preoptic area.Peptides 6:263–271.
Witkin, J. W., Paden, C. M., and Silverman, A. J. (1982). The luteinizing hormone-releasing hormone (LHRH) systems in the rat brain.Neuroendocrinology 35:429–438.
Witkin, J. W., O'Sullivan, H., and Silverman, A.-J. (1995). Novel associations among gonadotropin-releasing hormone neurons.Endocrinology 136:4323–4330.
Wormald, P. J., Abrahamson, M. J., Seeburg, P. H., Nikolics, K., and Millar, R. P. (1989). Prolactin-inhibiting activity of GnRH associated peptide in cultured human pituitary cells.Clin. Endocrinol. 30:149–155.
Wray, S., and Gainer, H. (1987). Effect of neonatal gonadectomy on the postnatal development of LHRH cell subtypes in male and female rats.Neuroendocrinology 45:413–419.
Wray, S., and Hoffman, G. (1986). A developmental study of the quantitative distribution of LHRH neurons within the central nervous system of postnatal male and female rats.J. Comp. Neurol. 252:522–531.
Wray, S., Zoeller, R. T., and Gainer, H. (1989). Differential effects of estrogen on luteinizing hormone-releasing hormone gene expression in slice explant cultures prepared from specific rat forebrain regions.Mol. Endocrinol. 3: 1197–1206.
Wu, T. I., Segal, A., Miller, G. M., Gibson, M. J., and Silverman, A. J. (1992).Fos expression in gonadotropin-releasing hormone neurons: Enhancement by steroid treatment and mating.Endocrinology 131:2045–2050.
Xia, L., Van Vugt, D., Alston, E. J., Luckhaus, J., and Ferin, M. (1992). A surge of gonadotropin-releasing hormone accompanies the estradiol-induced gonadotropin surge in the rhesus monkey.Endocrinology 131:2812–2820.
Yamada, H. (1994). Distribution of GnRH-receptors in murine forebrain by immunohistochemical technique.Endocrine Soc. Abstr. 447, p. 312.
Yang-Feng, T. L., Seeburg, P. H., and Francke, U. (1986). Human luteinizing hormone-releasing hormone gene (LHRH) is located on short arm of chromosome 8 (region 8p 11.2→p21).Somat. Cell Mol. Genet. 12:95–100.
Yen, T. J., Machlin, P. S., and Cleveland, D. W. (1988). Autoregulated instability of β-tubulin mRNAs by recognition of the nascent amino terminus of β-tubulin.Nature 334:580–585.
Young, L. S., Speight, A., Charlton, H. M., and Clayton, R. N. (1983). Pituitary gonadotropin-releasing hormone receptor regulation in the hypogonadotropic hypogonadal (hpg) mouse.Endocrinology 113:55–61.
Young, L. S., Detta, A., Clayton, R. N., Jones, A., and Charlton, H. M. (1985). Pituitary and gonadal function in hypogonadotrophic hypogonadal (hpg) mice bearing hypothalamic implants.J. Reprod. Fertil. 74:247–255.
Young, W. S., III, and Zoeller, R. T. (1987). Neuroendocrine gene expression in the hypothalamus:In situ hybridization histochemical studies.Cell. Mol. Neurobiol. 7:353–366.
Young, W. S., III, Mezey, E., and Siegal, R. E. (1986). Quantitativein situ hybridization histochemistry reveals increased levels of corticotropin-releasing factor mRNA after adrenalectomy.Neurosci. Lett. 70:198–203.
Yu, K. L., Yeo, T. T., Dong, K. W., Jakubowski, M., Lackner-Arkin, C., Blum, M., and Roberts, J. L. (1994). Second messenger regulation of mouse gonadotropin-releasing hormone gene expression in immortalized mouse hypothalamic GT1-3 cells.Mol. Cell. Endocrinol. 102:85–92.
Yu, W. H., Seeburg, P. H., Nikolics, K., and McCann, S. M. (1988). Gonadotropin-releasing hormone-associated peptide exerts a prolactin-inhibiting and weak gonadotropin-releasing activityin vivo.Endocrinology 123:390–395.
Zadina, J. E., Kastin, A. J., Fabre, L. A., and Coy, D. H. (1981). Facilitation of sexual receptivity in the rat by an ovulation-inhibiting analog of LHRH.Pharmacol. Biochem. Behav. 15:961–964.
Zoeller, R. T., and Young, W. S., III. (1988). Changes in cellular levels of messenger ribonucleic acid encoding gonadotropin-releasing hormone in the anterior hypothalamus of female rats during the estrous cycle.Endocrinology 123:1688–1689.
Zoeller, R. T., Seeburg, P. H., and Young, W. S., III, (1988)In situ hybridization histochemistry for messenger ribonucleic acid (mRNA) encoding gonadotropin-releasing hormone (GnRH): Effect of estrogen on cellular levels of GnRH mRNA in female rat brain.Endocrinology 122: 2570–2577.
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Sagrillo, C.A., Grattan, D.R., McCarthy, M.M. et al. Hormonal and neurotransmitter regulation of GnRH gene expression and related reproductive behaviors. Behav Genet 26, 241–277 (1996). https://doi.org/10.1007/BF02359383
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DOI: https://doi.org/10.1007/BF02359383