Abstract
Pinus hakkodensis has been considered as a hybrid betweenP. pumila andP. parviflora var.pentaphylla. Chloroplast DNA typing of this putative hybrid and hypothesized parental species in the Tanigawa Mountains, Japan, was conducted by PCR and single-strand conformation polymorphism (SSCP) of the intergenic spacer betweentrnL(UAA)3′ exon andtrnF(GAA) of cpDNA. Each of the hypothesized parental species collected from other mountain regions displayed a diagnostic SSCP pattern, whereas all morphological intermediates sampled in the Tanigawa Mountains had the SSCP pattern ofP. parviflora var.pentaphylla. Furthermore, some individuals classified on the basis of needle morphology as belonging toP. pumila in this mountain region showed the SSCP pattern ofP. parviflora var.pentaphylla. This may suggest that pollen-mediated uni-directional introgression fromP. parviflora var.pentaphylla toP. pumila occurs in the Tanigawa Mountains.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arnold, M.L. 1993.Iris nelsonii (Iridaceae): origin and genetic composition of a homoploid hybrid species. Amer. J. Bot.80: 577–583.
Brubaker, C.L., Koontz, J.A. andWendel, J.F. 1993. Bidirectional cytoplasmic and nuclear introgression in the New World cottons,Gossypium barbadense andG. hirsutum (Malvaceae). Amer. J. Bot.80: 1203–1208.
Doi, T. andMorikawa, K. 1929. An anatomical study of the leaves of the genusPinus. J. Dept. Agric. Kusyu Imp. Univ.2: 149–198.
Dorado, O., Rieseberg, L.H. andArias, D.M. 1992. Chloroplast DNA introgression in southern California sunflowers. Evolution46: 566–572.
Doyle, J.J. andDoyle, J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull.19: 11–15.
Govindaraju, D.R. andWagner, D.B. 1988. Chloroplast DNA variation within individual trees of aPinus banksiana-Pinus contorta sympatric region. Can. J. Forest. Res.18: 1347–1350.
Hagemann, R. andSchröder, M.B. 1989. The cytological basis of the plastid inheritance in angiosperms. Protoplasma152: 57–64.
Hayashi, Y. 1960. Taxonomical and Phytogeographical Study of Japanese Conifers. Norin-shuppan, Tokyo.
Isii, S. 1938. On the various forms ofPinus pumila distinguished by the structure of leaves with special refer ence to their distribution. J. Jap. Forest. Soc.20: 1–16.
isii, S. 1940a. On the various forms ofPinus pumila and other northern Japanese soft pines with special reference to their distribution (I). J. Jap. Forest. Soc.22: 581–586.
Isii, S. 1940b. On the various forms ofPinus pumila and other northern Japanese soft pines with special reference to their distribution (II). J. Jap. Forest. Soc.22: 651–656.
Isii, S. 1941a. On the various forms ofPinus pumila and other northern Japanese soft pines with special reference to their distribution (III). J. Jap. Forest. Soc.23: 1–7.
Isii, S. 1941b. On the various forms ofPinus pumila and other northern Japanese soft pines with special reference to their distribution (IV). J. Jap. Forest. Soc.23: 47–55.
Iwata, T. andKusaka, M. 1952. Coniferae Japonicae Illustratae. Sangyo Tosho, Tokyo.
Kron, K.A., Gawen, L.M. andChase, M.W. 1993. Evidence for introgression in azaleas (Rhododendron; Ericaceae): chloroplast DNA and morphological variation in a hybrid swarm on stone mountain, Georgia. Amer. J. Bot.80: 1095–1099.
Makino, T. andNemoto, K. 1931. Nippon-shokubutsusoran. 2nd ed., Syunyoudou, Tokyo.
Neale, D.B. andSederoff, R.R. 1988. Inheritance and evolution of conifer organelle genomes.In J.W. Hanover and D.E. Keathley, eds., Genetic Manipulation of Woody Plants, Plenum Press, New York, pp. 251–264.
Neale, D.B., Marshall, K.A. andSederoff, R.R. 1989. Chloroplast and mitchondrial DNA are paternally inherited inSequoia sempervirens D. Don Endl. Proc. Natl. Acad. Sci. USA86: 9347–9349.
Orita, M., Iwahara, H., Kanazawa, H., Hayashi, K. andSekiya, T. 1989a. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc. Natl. Acad. Sci. USA86: 2766–2770.
Orita, M., Suzuki, Y., Sekiya, T. andHayashi, K. 1989b. Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics5: 874–879.
Rieseberg, L.H. andSoltis, D.E. 1991. Phylogenetic consequences of cytoplasmic gene flow in plants. Evol. Trends Pl.5: 65–84.
Rieseberg, L.H. andWendel, J.F. 1993. Introgression and its consequences in plants.In R. Harrison, ed., Hybrid Zones and the Evolutionary Process, Oxford University Press, Oxford, pp. 70–109.
Satake, Y. 1989. Pinaceae.In Y. Satake, H. Hara, S. Watari and T. Tominari, eds., Wild Flowers of Japan, Woody Plants, Heibonsya, Tokyo, pp. 5–14.
Sears, B.B. 1980. Elimination of plastids during spermatogenesis and fertilization in the plant kingdom. Plasmid4: 233–255.
Taberlet, P., Gielly, L., Pautou, G. andBouvetet, J. 1991. Universal primers for amplification of three non-coding regions of chloroplast DNA. Pl. Mol. Biol.17: 1105–1109.
Tsukada, M. 1967. The last 12,000 years: a vegetational history of Japan I. Bot. Mag. Tokyo80: 323–336.
Wagner, D.B., Furnier, G.R., Saghai-Maroof, M.A., Williams, S.M. andDancik, B.P. 1987. Chloroplast DNA polymorphisms in lodgepole and jack pines and their hybrids. Proc. Natl. Acad. Sci. USA84: 2097–2100.
Wang, X. andSzmidt, A.E. 1994. Hybridization and chloroplast DNA variation in aPinus species complex from Asia. Evolution48: 1020–1031.
White, E.E. 1990. Chloroplast DNA inPinus monticola. Theor. Appl. Genet.79: 251–255.
Whittaker, R.H. 1956. Vegetation of the Great Smoky Mountains. Ecol. Monogr.26: 1–80.
Yahara, T., Yamaguchi, H. andYumoto, T. 1986. Biology of hybridization betweenFarfugium japonicum andF. hiberniflorum (Compositae).In K. Iwatsuki, P.H. Raven and W.J. Bock, eds., Modern Aspects of Species, University of Tokyo Press, Tokyo, pp. 183–193.
Yap, E.P.H. andMcGee, J.O'D. 1994. Mon-isotopic single-strand conformation polymorphism (SSCP) analysis of PCR products.In H.G. Griffin and A.M. Griffin, eds., PCR Technology: Current Innovations, CRC Press, Boca Raton, pp. 165–177.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Watano, Y., Imazu, M. & Shimizu, T. Chloroplast DNA typing by PCR-SSCP in thePinus pumila-P. parviflora var.pentaphylla complex (Pinaceae). J. Plant Res. 108, 493–499 (1995). https://doi.org/10.1007/BF02344239
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02344239