Summary
A HPLC system with post-column derivatization for the quantitative analysis of sugars in complex matrices is described. As reagent a 0.2% solution of thymol in concentrated sulfuric acid has been used. The reaction is sensitive with reducing as well as non reducing sugars whereas sugar alcohols are discriminated. With this reagent and separation of sugars at high pH values with an anion exchange column it is possible to detect sugars in the ng range. Hence, it is possible to characterize wines not only by their fructose and glucose content but also by differences in the distribution of the other not fermentable sugars like trehalose, arabinose, galacturonic and glucuronic acid.
Similar content being viewed by others
References
P. Jansson, O. Samuelson, Anal. Chem.39, 1156 (1967).
E. Martinson, O. Samuelson, J. Chromatogr.50, 429 (1970).
E. Paart, O. Samuelson, J. Chromatogr.85, 93 (1973).
O. Samuelson, Adv. in Chromatogr.10, 113 (1979).
K. W. Goulding, J. Chromatogr.103, 229 (1975).
R. Schwarzenbach, J. Chromatogr.117, 206 (1976).
H. Engelhardt, P. Orth, Chromatographia15, 91 (1982).
K. Aitzetmüller, J. Chromatogr.156, 354 (1978).
W. Böhme, PE Chromatogr. Newsl.8, 38 (1980).
H. Engelhardt, High Performance Liquid Chromatography, Springer, New York 1979.
H. Engelhardt, U. D. Neue, Chromatographia15, 403 (1982).
I. S. Krull, ed., Reaction Detection in Liquid Chromatography, M. Dekker, New York 1986.
R. L. Jolly, W. W. Pitt C. D. Scott, Anal. Biochem.,2, 300 (1969).
B. Kakac, Z. Veidelek, Handbuch der photmetrischen Analyse, VCH, Weinheim 1979.
H. Engelhardt, B. Lillig, HRC & CC8, 531 (1985).
H. Engelhardt, B. Klinkner, Fres. Z. anal. Chem.317, 687 (1984).
R. D. Rocklin, A. C. Pohl, J. Liq. Chromatogr.6, 1577 (1983).
J. A. Rendleman, Adv. in Chemistry117, 51 (1973).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Engelhardt, H., Ohs, P. Trace analysis of sugars by HPLC and post-column derivatization. Chromatographia 23, 657–662 (1987). https://doi.org/10.1007/BF02311495
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02311495