Abstract
The transformation of amorphous calcium phosphate into its crystalline form has been studiedin vitro under various conditions. The transformation was followed by changes in the pH and in the calcium and phosphate content of the solution and by changes in the Ca/P ratio and x-ray diffraction patterns of the solid phase. It was found that inorganic pyrophosphate markedly increased the time required for the transformation under the various conditions used. The addition of intestinal alkaline phosphatase abolished this retarding effect of pyrophosphate on the transformation.
It is proposed that pyrophosphate may be one of the factors that allows part of the bone mineral to persist in a non-crystalline state. The alkaline phosphatase of bone, by virtue of its pyrophosphatase activity, might be able to accelerate the transformation processin vivo.
Résumé
La transformation du phosphate calcique de la forme amorphe en forme cristalline a été étudiéein vitro dans différentes conditions. On a suivi cette transformation en étudiant la variation des paramètres suivants: le pH de la solution et sa teneur en calcium et en phosphate, ainsi que le rapport Ca/P et la courbe de diffraction aux rayons X de la phase solide. Le pyrophosphate inorganique augmente sensiblement le temps nécessaire à cette transformation dans les différentes conditions choisies. Cet effet s'annule lorsqu'on ajoute en plus du pyrophosphate de la phosphatase alcaline intestinale.
Les auteurs suggèrent que le pyrophosphate pourrait être l'un des facteurs qui permettent à une partie du minéral de l'os de demeurer dans un état non-cristallin. La phosphatase alcaline de l'os, grâce à son activité pyrophosphatasique, serait capable d'accélérer cette transormationin vivo.
Zusammenfassung
Die Umwandlung von amorphem in kristallines Calciumphosphat wurdein vitro unter verschiedenen Bedingungen studiert. Diese Umwandlung wurde folgendermaßen verfolgt: einerseits in der Lösung durch Änderung des pH's und des Calcium- und Phosphatgehaltes, andererseits in der soliden Phase durch Änderung des Ca/P-Verhältnisses, sowie des Röntgenstrahlendiffraktionsbildes.
Es konnte festgestellt werden, daß unter den verschiedenen Versuchsbedingungen die Anwesenheit von anorganischem Pyrophosphat die zur Umwandlung benötigte Zeit wesentlich verlängert. Wird noch intestinale alkalische Phosphatase zugesetzt, so wird die durch das Pyrophosphat verlängerte Umwandlungszeit aufgehoben.
Es wird vorgeschlagen, daß Pyrophosphat einer der Faktoren sein kann, der Teile des Knochenminerals in einem nicht kristallinen Zustand verbleiben läßt. Die alkalische Knochen-phosphatase könnte, dank ihrer Pyrophosphataseaktivität, eine Beschleunigung des Umwandlungsprozessesin vivo ermöglichen.
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Fleisch, H., Russell, R.G.G., Bisaz, S. et al. Influence of pyrophosphate on the transformation of amorphous to crystalline calcium phosphate. Calc. Tis Res. 2, 49–59 (1968). https://doi.org/10.1007/BF02279193
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DOI: https://doi.org/10.1007/BF02279193