Skip to main content
Log in

Restriction pattern analysis of genomic DNA ofFrankia isolates

  • Published:
Plant and Soil Aims and scope Submit manuscript

Summary

Total genomic DNAs ofFrankia isolates were subjected to restriction enzyme digestion and subsequent agarose gel electrophoresis. Restriction fragment banding patterns were unique for each isolate and may therefore be used as a method to distinguish between isolates which may be morphologically indistinguishable. This method might be useful for practical purposes such as tracing specificFrankia strains during field studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. An C S, Riggsby W S, Wills J W and Mullin B C 1983 Deoxyribonucleic acid base composition of 12Frankia isolates. Can. J. Bot. 61, 2859–2862.

    Google Scholar 

  2. An C S, Riggsby W S and Mullin B C 1984 Relationships ofFrankia isolates based on DNA homology studies. Int. J. Sys. Bacteriol.In press.

  3. Baker D, Pengelly W L and Torrey J G 1981 Immunochemical analysis of relationships among isolated frankiae (Actinomycetales). Int. J. Sys. Bacteriol. 31, 148–151.

    Google Scholar 

  4. Baker D and Torrey J G 1979 The isolation and cultivation of actinomycetous root nodule endophytes.In Symbiotic Nitrogen Fixation in the Management of Temperate Forests. Eds. J C Gordon, C T Wheeler and D A Perry Oregon State University, Corvallis, pp 38–56.

    Google Scholar 

  5. Benson D R, Buchholz S E and Hanna D G 1984 Identification ofFrankia strains by two-dimensional polyacrylamide gel electrophoresis. Appl. Env. Microbiol. 47, 489–494.

    Google Scholar 

  6. Burggraaf A J P and Shipton W A 1983 Studies on the growth ofFrankia isolates in relation to infectivity and nitrogen fixation (acetylene reduction). Can. J. Bot. 61, 2774–2782.

    Google Scholar 

  7. Burggraaf A J P and Valstar J 1984 Heterogeneity within Frankia sp. LDAgpl studied among clones and reisolates. Plant and Soil 78, 29–43.

    Article  Google Scholar 

  8. Callaham D, Tredici P D and Torrey J G 1978 Isolation and cultivationin vitro of the actinomycete causing root nodulation inComptonia. Science 199, 899–902.

    Google Scholar 

  9. Crameri R, Hintermann G and Hütter R 1983 Deoxyribonucleic acid restriction endonuclease fingerprint characterization of actinomycete strains. Int. J. Syst. Bacteriol. 33, 652–655.

    Google Scholar 

  10. Hintermann G, Crameri R, Kieser T and Hütter R 1981 Restriction analysis of theStreptomyces glaucescens genome by agarose gel electrophoresis. Arch. Microbiol. 130, 218–222.

    Article  Google Scholar 

  11. Lalonde M and Calvert E H 1979 Production ofFrankia hyphae and spores as an infective inoculant forAlnus species.In Symbiotic Nitrogen Fixation in the Management of Temperate Forests. Eds. J C Gordon, C T Wheeler and D A Perry. Oregon State University, Corvallis, pp 95–110.

    Google Scholar 

  12. Lechevalier M P 1984 Taxonomy of the genusFrankia. Plant and Soil 78, 1–6.

    Article  Google Scholar 

  13. Lechevalier M P and Lechevalier H A 1979 The taxonomic position of the actinomycetic endophytes.In: Symbiotic Nitrogen Fixation in the Management of Temperate Forests. Eds. J C Gordon, C T Wheeler and D A Perry, Oregon State University, Corvallis, pp 111–122.

    Google Scholar 

  14. Lechevalier M P and Lechevalier H A 1983 Taxonomy ofFrankia. In Proceedings of the Fifth International Symposium on Actinomycete Biology. Ed. L F Bojalil. Oaxtepec, Mexico.

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

An, C.S., Riggsby, W.S. & Mullin, B.C. Restriction pattern analysis of genomic DNA ofFrankia isolates. Plant Soil 87, 43–48 (1985). https://doi.org/10.1007/BF02277646

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02277646

Key words

Navigation