Summary
A high performance liquid chromatography system for the analysis of photosynthetic pigments is presented. The method employs an octadecylsilica stationary phase, a programmed quaternary mobile phase consisting of mixtures of methanol, acetonitrile, water and hexane, and a photodiode array detector. Carotenoids and chlorophylls are rapidly analysed in a single chromatographic separation. Thecis-trans isomers of most carotenoids are separated by this method.
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Francis, G.W., Huseby, B. & Andersen, Ø.M. An improved HPLC system for the analysis of photosynthetic pigments. Chromatographia 35, 189–192 (1993). https://doi.org/10.1007/BF02269701
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DOI: https://doi.org/10.1007/BF02269701