Summary
For the evaluation of reversed phase packing materials a mixture of acetylacetone, I-nitronaphthalene and naphthalene is proposed. This will reveal the usual optimum kinetic chromatographic parameters (the naphthalene peak), the degree of activity or endcapping status of the column (the ratio of the I-nitronaphthalene and naphthalene retention times) and trace metal activity (the shape and intensity of the acetylacetone peak).
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References
P. Bristow, J. H. Knox, Chromatographia,10, 279 (1977).
H. G. Genieser, D. Gabel, B. Jastorff, J. Chromatogr.244, 368 (1982).
R. P. W. Scott, P. Kucera, J. Chromatogr.,142, 213 (1977).
R. Eksteen, H. E. Schwartz, B. L. Karger, HPLC Symp. Avignon 1981, poster session personal communication.
N. H. C. Cooke, K. Olsen, J. Chromatogr. Sci.,18, 512 (1980).
F. Rabel, C. Lancaster, J. Bickler, Pittsburg Conference 1980.
C. Dewaele, P. Mussche, M. Verzele, J. High. Resoln. Chromatogr./Chromatogr. Commun.,5, (11), 616 (1982).
M. De Potter, J. Gheysels, M. Verzele, J. High. Resoln. Chromatogr./Chromatogr. Commun.,9, 151 (1978).
M. Verzele, C. Dewaele, J. Chromatogr.,217, 399 (1981).
S. Abbott, R. Simpson, Varian Instrument Group. “Spherical 3 and 5 μm Reversed Phase Materials”.
K. van Oeteren, C. Dewaele, M. Verzele, to be published.
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Verzele, M., Dewaele, C. The evaluation of “reversed phase” high-performance liquid chromatography packing materials. Chromatographia 18, 84–86 (1984). https://doi.org/10.1007/BF02268462
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DOI: https://doi.org/10.1007/BF02268462