Summary
HPLC retention data for a number of flavonoid compounds have been evaluated on normal phase silica and cyano colums according to the Soczewinski-Snyder (S.-S.) model. On the silica phase most of the flavones examined exhibited asymetrical peak shapes indicating strong and mixed retention mechanisms; on the cyano phase the intercept of the S.-S. relationship was independent of any hydroxy substitution pattern or of molecular planarity. A similar behaviour, but with different intercept values, was observed with coumarin and quinone compounds.
Similar content being viewed by others
References
J. Daigle, E. J. Conkerton, J. Liq. Chromatogr.11, 309 (1988).
K. W. Nicholls, B. A. Bohm, Biochem. Syst. Ecol.10, 225 (1982).
J. F. Goossens, A. J. Van Laere, J. Chromatogr.267, 439 (1983).
F. Siewek, R. Galensa, J. Chromatogr.294, 395 (1984).
K. V. Casteele, H. Geiger, C. S. Van Sumere, J. Chromatogr.240, 81 (1982).
F. A. T. Barberan, F. Tomas, L. Hernandez, F. Ferreres, J. Chromatogr.347, 443 (1985).
F. Dondi, Y. D. Kahie, G. Lodi, P. Reschiglian, C. Pietrogrande, G. P. Cartoni, Chromatographia23, 844 (1987).
F. Dondi, G. Bio, Y. D. Kahie, G. Lodi, C. Pietrogrande, P. Reschiglian, Chromatographia25, 423 (1988).
F. Dondi, Y. D. Kahie, G. Blo, C. Pietrogrande, P. Reschiglian, J. Chromatogr.461, 281 (1989).
F. Dondi, G. Grasshini-Strazza, Y. D. Kahie, G. Lodi, C. Pietrograde, P. Reschiglian, C. Bighi, J. Chromatogr.462, 205 (1989).
W. E. Hammers, C. H. Kos, W. K. Brederode, C. L. De Ligny, J. Chromatogr.168, 9 (1979).
L. R. Snyder, J. L. Glajch, J. Chromatogr.248, 165 (1982).
E. Soczewinski, T. Dzido, Chroamtographia22, 25 (1986).
A. Hussain, R. J. Hurtubise, H. F. Silver, Chromatogr.252, 21 (1982).
M. Verzele, F. Van Damme, J. Chromatogr.393, 25 (1987).
M. C. Pietrogrande, F. Dondi, G. Blo, P. A. Borea, C. Bighi, J. Liq. Chromatogr.11, 1313 (1988).
S. M. Petrovic, L. A. Kolarov, E. S. Traljic, J. A. Petrovic, Anal. Chem.54, 934 (1982).
J. Thomas, J. Chromatogr.404, 73 (1987).
M. C. Etter, Z. Urbanczyk-Lipkowska, S. Baer, P. F. Barbara, J. Mol. Structure144, 155 (1986).
E. L. Weiser, A. W. Salotto, S. M. Flach, L. R. Snyder, J. Chromatogr.303, 1 (1984).
W. T. Cooper, P. L. Smith, J. Chromatogr.355, 57 (1986).
M. L. Bieganowska, K. Glowniak, Chromatographia,25, 111 (1988).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pietrogrande, M.C., Bighi, C., Blo, G. et al. Retention behaviour of selected flavonoid compounds in NP-HPLC. Chromatographia 27, 625–627 (1989). https://doi.org/10.1007/BF02258991
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02258991