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Effects of arbuscular mycorrhiza (AM) on health ofLinum usitatissimum L. infected by fungal pathogens

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Abstract

Effects of arbuscular mycorrhizal (AM) symbiosis on health ofLinum usitatissimum infected by fungal pathogens were investigated exemplarily. Physiological and biochemical analyses were done to explain the mechanisms underlying the AM effects. AM plants showed increased resistance against the wilt pathogen (Fusarium oxysporum f. sp.lini), the level of this effects depended on the plant cultivars which all showed the same level of root colonization by arbuscular mycorrhizal fungi (AMF). In contrary to that, AM plants were highly susceptible against the shoot pathogenOidium lini, but they suffered less than non-AM plants in terms of shoot fresh weight, CO2 assimilation and content of sucrose in shoot apex. This indicates that AM not only activates resistance mechanisms but also can induce tolerance against pathogens. The concentration of phytohormones such as auxin- and gibberellin-like substances were increased in shoots of AM plants. In roots the ethylene production was increased, too. Furthermore the content and composition of free sterols were highly altered in leaves of AM plants. Root infection by AMF caused an increased respiratory activity and a reduced degree of DNA methylation, but both modifications only occurred in infected root parts indicating an increasing gene activity. The presented results suggest that nearly all parts of a plant are influenced by AM but not in the same manner. In the case of mildewed linseed the effect of AM on plant health was impressing, it indicates that AM has an ability to induce tolerance.

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References

  • Allen M F, Moore J T S and Christensen M 1982 Phytohormone changes inBouteloua gracilis infected by vesicular-arbuscular mycorrhizae. II. Altered levels of gibberellin-like substances and abscisic acid in the host plant. Can. J. Bot. 60, 468–471.

    Google Scholar 

  • Baas R, Van der Werf A and Lambers H 1989 Root respiration and growth inPlantago major as affected by vesicular-arbuscular mycorrhizal infection. Plant Physiol. 91, 227–232.

    Google Scholar 

  • Benhamou N, Fortin J A, Hamel C, St-Arnaud M and Shatilla A 1994 Resistance reponses of mycorrhizal Ri T-DNA-transformed carrot roots to infection byFusarium oxysporum f.sp.chrysanthemi. Phytopathol. 84, 958–968.

    Google Scholar 

  • Caron M, Fortin J A and Richard C 1986 Effect of phosphorus concentration andGlomus intraradices onFusarium crown and root rot of tomatoes. Phytopathol. 76, 942–946.

    Google Scholar 

  • Cooper K M and Lösel D M 1978 Lipid physiology of vesicular-arbuscular mycorrhiza. I. Composition of lipids in roots of onion, clover and ryegrass infected withGlomus mosseae. New Phytol. 80, 143–151.

    Google Scholar 

  • Dehne H W 1977 Untersuchungen über den Einfluß der endotrophen Mycorrhiza auf dieFusarium-Welke an Tomate und Gurke. Dissertation Univ. Bonn, Germany.

  • Dehne H W 1987 Zur Bedeutung der vesikulär-arbuskulären (VA) Mykorrhiza für die Pflanzengesundheit. Habil. Univ. Hannover, Germany.

    Google Scholar 

  • Dehne H W und Schönbeck F 1979 Untersuchungen zum Einfluß der endotrophen Mykorrhiza auf Pflanzenkrankheiten. II. Phenolstoffwechsel und Liginifizierung. Phytopatol. Z. 5, 210–216.

    Google Scholar 

  • Dehne H W, Schönbeck F und Baltruschat H 1978 Untersuchungen zum Einfluß der endotrophen Mykorrhiza auf Pflanzenkrankheiten. III. Chitinase-Aktivität und Ornithinzyklus. Z. Pflkrankh. Pflschutz. 85, 666–678.

    Google Scholar 

  • Dehne H W and Backhaus G H 1986 The use of vesicular-arbuscular mycorrhizal fungi in plant production. I. Inoculum production. Z. PflKrankh. PflSchutz 93, 415–424

    Google Scholar 

  • Dixon R A, Harrison M J and Lamb C J 1994 Early events in the activation of plant defense responses. Annu. Rev. Phytopathol. 32, 479–501.

    Article  Google Scholar 

  • Drüge U und Schönbeck F 1992 Effect of vesicular-arbuscular mycorrhizal infection on transpiration, photosynthesis and growth of flax (Linum usitatissimum L.) in relation to cytokinin levels. J. Plant Physiol. 141, 40–48.

    Google Scholar 

  • Dugassa G D 1995 Zum Einfluß der vesikulären-arbuskulären Mycorrhiza auf Wachstum, Entwicklung und Gesundheit von Lein (Linum usitatissimum L.). Dissertation Univ. Hannover, Germany.

  • Dugassa G D, Grunewaldt-Stöcker G and Schönbeck F 1995 Growth ofGlomus intraradices and its effect on linseed (Linum usitatissimum L.) in hydroponic culture. Mycorrhiza 5, 279–281.

    Google Scholar 

  • Fretz T A, Dunham C W and Woodmansee C W 1970 A gas chromathographic procedure for determining soluble carbohydrates extracted from leaf tissue ofllex opaca Ait. Cv. ‘Miss Helen’. J. Am. Soc. Hort. Sci. 95, 99–102.

    Google Scholar 

  • Green D 1983 Soluble sugar changes occumng during cold hardening of spring wheat, fall rye and alfalfa. Can. J. Plant Sci. 63, 415–420.

    Google Scholar 

  • Harley J L and Smith S E 1983 Mycorrhiza Symbiosis. Academic Press, London, UK. 483 p.

    Google Scholar 

  • Hemberg T and Tillberg E 1980 The influence of the extraction procedure on yield of indole-3-acetic acid in plant extracts. Physiol. Plant. 50, 176–182.

    Google Scholar 

  • Hendrix J W 1970 Sterols in growth and reproduction of fungi. Annu. Rev. Phytopathol. 8, 111–130.

    Article  Google Scholar 

  • Howard A 1943 An Agricultural Testament. Oxford University Press, Geoffrey Cumberlege, London, UK.

    Google Scholar 

  • Hunter W T 1993 Ethylene production by root nodules and effect of ethylene on nodulation inGlycine max. Appl. Environ. Microbiol. 59, 1947–1950.

    Google Scholar 

  • Klaas M, Manorama C J, Crowell D N and Amasino R M 1989 Rapid induction of genomic demethylation and T-DNA gene expression in plant cells by 5-azacytosine derivatives. Plant Mol. Biol. 12, 413–423.

    Article  Google Scholar 

  • Koide R T and Schreiner R P 1992 Regulation of the vesicular-arbuscular mycorrhizal symbiosis. Annu. Rev. Plant Physiol. 43, 557–581.

    Article  Google Scholar 

  • Kombrink E and Somssich I E 1995 Defense responses of plants to pathogens. Adv. Bot. Res. 21, 1–34.

    Google Scholar 

  • Kraska T 1996 Vergleichende Untersuchungen von Resistenzinduktoren, deren Wirkungsweisen und dem Einflu\ auf die DNA-Methylierung. Dissertation Univ. Hannover, Germany.

  • Lösel D M 1991 Synthesis and functioning of membrane lipids in fungi and infected plants. Pestic. Sci. 32, 353–362

    Google Scholar 

  • Miyamoto K, Ueda J and Kamisaka S 1993 Gibberellin-enhanced sugar accumulation in growing subhooks of etiolatedPisum sativum seedlings. Effects of gibberellic acid, indoleacetic acid and cycloheximide on invertase activity, sugar accumulation and growth. Physiol. Plant. 88, 301–306.

    Article  Google Scholar 

  • Morandi D, Bailey J A and Gianinazzi-Pearson V 1984 Isoflavonoid accummulation in soybean roots infected with vesicular-arbuscular mycorrhizal fungi. Physiol. Plant Pathol. 24, 357–364.

    Google Scholar 

  • Mucharromah and Kuć J 1995 The effect of sterols on the compatibility or incombatibilty of potato tuber discs and foliage toPhytophthora infestans andHelminthosporium carbonum, and the induction of resistance by arachidonic acid. Physiol. Mol. Plant Pathol. 47, 1–12.

    Article  Google Scholar 

  • Nagy S, Nordby H E and Nemec S 1980 Composition of lipids in roots of six citrus cultivars infected with the vesicular-arbuscular mycorrhizal fungus,Glomus mosseae. New Phytol. 85, 377–384.

    Google Scholar 

  • Newton A C 1989 Measuring the sterol content of barley leaves infected with powdery mildew as a means of assessing partial resistance toErysiphe graminis f.sp.hordei. Plant Pathol. 38, 534–540.

    Google Scholar 

  • Rademacher W and Graebe J E 1984 Isolation and analysis by gasliquid chromatography of auxins, gibberellins, cytokinins, and abscisic acid from a single sample of plant material. Ber. Dtsch. Bot. Ges. 97, 75–85.

    Google Scholar 

  • Rogers S O and Bendich A J 1985 Extraction of DNA from miligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol. Biol. 5, 69–75.

    Article  Google Scholar 

  • Schönbeck F und Schinzer U 1972 Untersuchungen über den Einfluß der endotrophen Mykorrhiza auf die TMV-Läsionenausbildung inNicotiana tabacum L. var. Xanthinc. Phytopath. Z. 73, 78–80.

    Google Scholar 

  • Schönbeck F 1979 Endomycorrhiza in relation to plant diseases.In Soil-Borne Plant Pathogens. Eds. B Schippers and W Gams. pp 272–280. Acadamic Press, London, UK.

    Google Scholar 

  • Sembdner G, Schneider G und Schreiber K 1987 Methoden zur Pflanzenhormonanalyse. Springer Verlag, Berlin, Germany.

    Google Scholar 

  • Smith S E and Gianinazzi-Pearson V 1988 Physiological interactions between symbionts in vesicular-arbuscular mycorrhizal plants. Annu. Rev. Plant Physiol. Mol. Biol. 39, 211–244.

    Article  Google Scholar 

  • Snellgrove R C, Splittstosser W E, Stribley D P and Tinker P B 1982 The distribution of carbon and the demand of the fungal symbiont in leek plants with vesicular-arbuscular mycorrhizas. New Phytol. 92, 75–87.

    Google Scholar 

  • St-Arnaud M, Hamel C, Vimard B, Caron M and Fortin J A 1995 Altered growth ofFusarium oxysporum f. sp.chrysanthemi in an in vitro dual culture system with the vesicular arbuscular mycorrhizal fungusGlomus intraradices growing onDaucus carota transformed roots. Mycorrhiza 5, 431–438

    Google Scholar 

  • Stahl E 1900 Der Sinn der Mykorrhizabildung. Jahrb. Wiss. Bot. 34, 539–667.

    Google Scholar 

  • STSC 1991 Statgraphics-Statistical Graphics System. Stat. Graphics Corp., Rockville, MD, USA.

    Google Scholar 

  • Stoessl A and Venis M A 1970 Determination of submicrogram levels of indole-3-acetic acid: A new, highly specific method. Anal. Biochem. 34, 344–351.

    Article  PubMed  Google Scholar 

  • Thompson J P 1994 Inoculation with vesicular-arbuscular mycorrhizal fungi from cropped soil overcomes long-fallow disorder of linseed (Linum usitatissimum L.) by improving P and Zn uptake. Soil Biol. Biochem. 26, 1133–1143.

    Article  Google Scholar 

  • Tosi L, Giovannetti M, Zazzerini A and Sbrana C 1993 Interactions betweenPlasmopara helianthi and arbuscular mycorrhizal fungi in sunflower seedlings susceptible and resistant to downy mildew. Phytopath. Medit. 32, 106–114.

    Google Scholar 

  • Toth R, Toth D, Starke D and Smith D R 1990 Vesicular-arbuscular mycorrhizal colonization inZea mays affected by breeding for resistance to fungal pathogens. Can. J. Bot. 68, 1039–1044.

    Google Scholar 

  • Von Alten H, von Reichenbach H, Steiner U und Schönbeck F 1992 Möglicher Nutzen der VA-Mykorrhiza im Leinanbau. Mitt. Biol. Bundesanst., Berlin, Heft 283, p 333.

  • Von Reicheribach G H and Schönbeck F 1995 Influence of VA mycorrhiza on drought tolerance of flax (Linum usitatissimum L.). I. Influence of VAM on growth and morphology of flax and on physical parameters of the soil. Angew. Bot. 69, 49–54.

    Google Scholar 

  • West H M 1995 Soil phosphate status modifies response of mycorrhizal and non-mycorrhizalSenecio vulgaris L. to infection by the rust,Puccinia lagenophorae Cooke. New Phytol. 129, 107–116.

    Google Scholar 

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Authors and Affiliations

  1. Institut für Pflanzenkrankheiten und Pflanzenschutz, Universität Hannover, Herrenhäuser Str. 2, D-30419, Hannover, Germany

    G. D. Dugassa, H. von Alten & F. Schönbeck

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  1. G. D. Dugassa
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  2. H. von Alten
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  3. F. Schönbeck
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Correspondence to H. von Alten.

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Dugassa, G.D., von Alten, H. & Schönbeck, F. Effects of arbuscular mycorrhiza (AM) on health ofLinum usitatissimum L. infected by fungal pathogens. Plant Soil 185, 173–182 (1996). https://doi.org/10.1007/BF02257522

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  • DOI: https://doi.org/10.1007/BF02257522

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