Summary
Two distinct groups of granules are present in the chick heterophil leucocytes: a) large spindle shaped granules; b) small elongated granules. The acid hydrolases (acid phosphatase and arylsulfatase) occur only in granules of the first group, which therefore can be regarded as lysosomes, whilst the granules of the second group are always negative. The lead phosphate deposits are found in different zones during various maturation stages of the large spindle shaped granules: at first they occur in their periphery, then in form of clusters throughout the matrix, finally they appear exclusively in the center of the granule. The results obtained have also been discussed in comparison with data on mammalian heterophil leucocytes.
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Baggiolini, M., Hirsch, J. G., Duve, C. de: Resolution of granules from rabbit heterophil leukocytes into distinct populations by zonal sedimentation. J. Cell Biol.40, 529–541 (1969).
Bainton, D. F., Farquhar, M. G.: Enzyme composition of polymorphonuclear leukocyte granules. J. Cell Biol.31, 8A (1966).
—: Differences in enzyme content of azurophil and specific granules of polymorphonuclear leukocytes. I. Histochemical staining of bone marrow smears. J. Cell Biol.39, 286–298 (1968).
—: Differences in enzyme content of azurophil and specific granules of polymorphonuclear leukocytes. II. Cytochemistry and electron microscopy of bone marrow cells. J. Cell Biol.39, 299–317 (1968).
Duve, C. de: The lysosome concept. In: Ciba Foundation Symposium on Lysosomes (A. V. S. de Reuck and M. P. Cameron, eds., 1. Boston: Little, Brown & Co. (1963).
Gomori, G.: Histochemistry of human esterases. J. Histochem. Cytochem.3, 479–484 (1955).
Hirsch, J. G., Cohn, Z. A.: Digestive and autolytic functions of lysosomes in phagocytic cells. Fed. Proc.23, 1023 (1964).
Hopsu-Havu, V. K., Arstila, A. U., Helminen, H. J., Kalimo, H. O., Clenner, G. G.: Improvements in the method for electron microscopic localization of arylsulfatase activity. Histoohemie8, 54–64 (1967).
Novikoff, A. B., Essner, E., Quintana, N.: Golgi apparatus and lysosomes. Fed. Proc.23, 1010–1022 (1964).
Osculati, F., Carosi, G.: Il granulocito eterofilo degli Uccelli al microscopio elettronico. Istituto Lombardo (Rend. Sc.) B101, 415–429 (1967).
Seeman, P. M., Palade, G. E.: Acid phosphatase localization in rabbit eosinophils. J. Cell Biol.34, 745–756 (1967).
Wetzel, B. K., Spicer, S. S., Horn, R. G.: Fine structural localization of acid and alkaline phosphatases in cells of rabbit blood and bone marrow. J. Histochem. Cytochem.15, 311–334 (1967).
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Osculati, F. Fine structural localization of acid phosphatase and arylsulfatase in the chick heterophil leucocytes. Z.Zellforsch 109, 398–406 (1970). https://doi.org/10.1007/BF02226911
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DOI: https://doi.org/10.1007/BF02226911