Abstract
Chlorophyll, protein, DNA and RNA concentrations in cultures ofCyclotella nana (Hustedt) andDunaliella tertiolecta (Butcher) were higher in blue light and lower in green light than in white light of the same intensity. Total carotenoid concentrations were highest in green light. Total pigment concentrations were highest in cells grown in blue light, lowest in those from green light. The relative changes in pigment concentrations associated with differences in spectral composition of the radiation are comparable to those often observed in phytoplankton from various depths in stratified natural water. Light adaptation in algae as a response to light quality is suggested. The chemical composition of plankton algae can be expected to vary considerably with depth.
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Literature cited
Bidwell, R. G. S., R. A. Barr andF. C. Steward: Protein synthesis and turn-over in cultured plant tissue: sources of carbon for synthesis and the fate of the protein breakdown products. Nature, Lond.203, 367–373 (1964).
Brody, M. andR. Emerson: The effect of wavelength and intensity of light on the proportion of pigments inPorphyridium cruentum. Am. J. Bot.46, 433–440 (1959).
Fujita, Y. andA. Hattori: Photochemical interconversion between precursors of phycobilin chromoproteids inTolypothrix tenuis. Pl. Cell Physiol., Tokyo3, 209–220 (1962).
Fulton, J. D., O. D. Kennedy, H. Seki andK. Stephens: Biological, chemical and physical observations in Saanich Inlet, Vancouver Island, British Columbia, 1968. Manuscript Rep. Fish., Res. Bd Can. No.1018, 109 pp. 1969.
Goodwin, T. W.: Carotenoids.In: Modern methods of plant analysis, Vol. 3, pp 272–311. Ed. byK. Paech andM. Tracey. Heidelberg: Springer-Verlag 1955.
Holm-Hansen, O., W. H. Sutcliffe, Jr. andJ. Sharp: Measurement of deoxyribonucleic acid in the ocean and its ecological significance. Limnol. Oceanogr.13, 507–513 (1968).
Jeffrey, S. W.: Paper chromatographic separation of chlorophylls and carotenoids from marine algae. Biochem. J.80, 336–342 (1961).
— Purification and properties of chlorophyllc fromSargassum flavicans. Biochem. J.86, 313–318 (1963).
Jerlov, N. G.: Light. General introduction.In: Marine ecology, pp 95–102. Ed. byO. Kinne. London: Wiley-Interscience 1970.
Jones, L. W. andJ. Meyers: Pigment variations inAnacystis nidulans induced by light of selected wavelengths. J. Phycol.1, 7–14 (1965).
Jorgensen, E. G. andE. Tteemann Nielsen: Adaptation in plankton algae. Memorie Ist. ital. Idrobiol.18 Suppl., 37–46 (1965).
Kowallik, W.: Die Proteinproduktion vonChlorella im Licht verschiedener Wellenlängen. Planta64, 191–200 (1965).
Lowry, O. H., N. J. Rosebrough, A. L. Farr andR. J. Randall: Protein measurement with the Folin phenol reagent. J. biol. Chem.193, 265–275 (1951).
Parsons, T. R. andJ. D. H. Strickland: Discussion of spectrophotometric determination of marine plant pigments with revised equations for ascertaining chlorophylls and carotenoids. J. mar. Res.21, 155–163 (1963).
Pirson, A. andW. Kowallik: Spectral responses to light by unicellular plants. Photochem. Photobiol.3, 489–497 (1964).
Raghavan, V.: Ribonucleic acid and protein changes in the subcellular components of the gametophytes ofPteridium aquiiinum during growth in red and blue light. Physiologia Pl.21, 1020–1028 (1968).
Ruppel, H. G.: Untersuchungen über die Zusammensetzung vonChlorella bei Synchronisation im Licht-Dunkel-Wechsel. Flora, Jena152, 113–138 (1962).
Ryther, J. H. andD. W. Menzel: Light adapation by marine phytoplankton. Limnol. Oceanogr.4, 492–497 (1959).
Saijo, Y. andS. Ichimura: Some considerations on photosynthesis of phytoplankton from the point of view of productivity measurement. J. oceanogr. Soc. Japan20, 687–693 (1962).
Schneider, W. C.: Determination of nucleic acids in tissues by pentose analysis.In: Methods in enzymology, Vol. 3, pp 680–684. Ed. byS. P. Colwick andN. O. Kaplan. New York. Academic Press 1957.
Smith, J. H. C. andA. Benitez: Chlorophylls: analysis in plant materials.In: Modern methods of plant analysis, vol.4, pp 142–196. Ed. byK. Paech andM. Tracey. Heidelberg: Springer-Verlag 1955.
Sokawa, Y. andE. Hase: Effects of light on the deoxyribonucleic acid formation and cellular division inChlorella protothecoides. Pl. Cell Physiol., Tokyo 8, 509–522 (1967).
Steemann Nielsen, E. andV. K. Hansen: Influence of surface illumination on phytoplankton photosynthesis in Danish waters (56° N) throughout the year. Physiologia Pl.14, 593–613 (1961).
— andE. G. Jorgensen: The adaptation of plankton algae. I. General part. Physiologia Pl.21, 401–413 (1968).
Strickland, J. D. H. andT. R. Parsons: A manual of sea-water analysis (with special reference to the more common micronutrients and to particulate organic material). Bull. Fish. Res. Bd Can.125, 1–195 (1965).
Voskresenskaya, N. P. andE. P. Nechaeva: Effect of blue, red, and green light on the content of prtein, nucleic acids and chlorophyll in young barley plants. Fiziologiya Rast.14, 299–308 (1967).
Wallen, D. G., andG. H. Geen: Light quality in relation to growth, photosynthetic rates and carbon metabolism in two species of marine plankton algae. Mar. Biol.10, 34–43 (1971).
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Communicated byT. R. Parsons, Nanaimo
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Wallen, D.G., Geen, G.H. Light quality and concentration of proteins, RNA, DNA and photosynthetic pigments in two species of marine plankton algae. Mar. Biol. 10, 44–51 (1971). https://doi.org/10.1007/BF02026765
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DOI: https://doi.org/10.1007/BF02026765