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Evaluation of an antigen-capture enzyme immunoassay for rapid diagnosis ofMycoplasma pneumoniae infection

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Abstract

A new enzyme immunoassay (EIA; Enzygost) for rapid detection ofMycoplasma pneumoniae antigen was evaluated in 51 young adults with acute respiratory infection. The EIA results using sputa and nasopharyngeal aspirates were compared with those of serological antibody tests, culture and a DNA probe. In sputum the sensitivity of the EIA ranged from 40 % to 81 % and the specificity from 64 % to 100 %, depending on the reference method. In nasopharyngeal aspirates the sensitivity was well below 20 %, but the test was nearly 100 % specific.

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Authors and Affiliations

  1. National Public Health Institute, Mannerheimintie 166, 00300, Helsinki, Finland

    M. Kleemola & R. Räty

  2. Central Military Hospital, Helsinki, Finland

    J. Karjalainen

  3. Behringwerke AG, Marburg, Germany

    W. Schuy

  4. Institute for Medical Microbiology and Hygiene, University Hospital, Freiburg, Germany

    B. Gerstenecker & E. Jacobs

Authors
  1. M. Kleemola
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  2. R. Räty
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  3. J. Karjalainen
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  4. W. Schuy
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  5. B. Gerstenecker
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  6. E. Jacobs
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Kleemola, M., Räty, R., Karjalainen, J. et al. Evaluation of an antigen-capture enzyme immunoassay for rapid diagnosis ofMycoplasma pneumoniae infection. Eur. J. Clin. Microbiol. Infect. Dis. 12, 872–875 (1993). https://doi.org/10.1007/BF02000413

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  • DOI: https://doi.org/10.1007/BF02000413

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