Skip to main content
SpringerLink
Log in

Diagnosis of Lyme borreliosis by an enzyme immunoassay detecting immunoglobulin G reactive to purifiedBorrelia burgdorferi cell components

  • Article
  • Published:
European Journal of Clinical Microbiology and Infectious Diseases Aims and scope Submit manuscript

Abstract

An enzyme immunoassay (EIA) developed for the diagnosis of Lyme borreliosis was tested for its specificity and sensitivity in detecting IgG antibodies in patients at various stages of the disease. The EIA is based on a detergent extract ofBorrelia burgdorferi which contains 12 proteins of defined molecular weights fromBorrelia burgdorferi. The assay showed a specificity of 100 % in control sera from 64 healthy individuals, using a cut-off optical density value of 0.13 (\(\bar x \pm 3SD\)). The sensitivity was 100 % using sera from 22 Swedish patients with late stage Lyme borreliosis and 43 % using sera from 30 patients with the initial stage of the disease. The reactivity of the sera against whole cell preparations, the outer surface proteins OspA and OspB, and the flagella ofBorrelia burgdorferi was also tested and compared with the EIA. No cross-reactivity with treponemal antigens was observed when using the EIA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Burgdorfer W, Barbour AG, Hayes SF, Benach JL, Grunwaldt E, Davis, JP: Lyme disease — a tick borne spirochetosis? Science 1983, 216: 1317–1319.

    Google Scholar 

  2. Steere AC, Grodzicki RL, Kornblatt AN, Craft JE, Barbour AG, Burgdorfer W, Schmid GP, Johnson E, Malawista SE: The spirochetal etiology of Lyme disease. New England Journal of Medicine 1983, 308: 733–740.

    PubMed  Google Scholar 

  3. Johnson RC, Schmid GP, Hyde FW, Steigerwalt AG, Brenner DJ:Borrelia burgdorferi sp. nov. Etiologic agent of Lyme disease. International Journal of Systematic Bacteriology 1984, 4: 496–497.

    Google Scholar 

  4. Steere AC, Bartenhagen NH, Craft JE, Hutchinson GJ, Newman JH, Rahn DW, Sigal LH, Spieler PN, Stenn KS, Malawista SE: The early clinical manifestations of Lyme disease. Annals of Internal Medicine 1983, 99: 76–82.

    PubMed  Google Scholar 

  5. Åsbrink E, Hovmark A: Successful cultivation of spirochetes from skin lesions of patients with erythema chronicum migrans Afzelius and acrodermatitis chronica atrophicans. Acta Pathologica, Microbiologica et Immunologica Scandinavica (B) 1985, 93: 161–163.

    Google Scholar 

  6. Stiernstedt G, Granström M, Hederstedt B, Sköldenberg B: Diagnosis of spirochetal meningitis by enzyme-linked immunosorbent assay and indirect immunosorbent assay in serum and cerebrospinal fluid. Journal of Clinical Microbiology 1985, 21: 819–825.

    PubMed  Google Scholar 

  7. Craft JE, Grodzicki RL, Steere AC: Antibody response in Lyme disease: evaluation of diagnostic tests. Journal of Infectious Diseases 1984, 149: 789–795.

    PubMed  Google Scholar 

  8. Russell H, Sampson JS, Schmid GP, Wilkinson HW, Plikaytis B: Enzyme-linked immunosorbent assay and indirect immunofluorescens assay for Lyme disease. Journal of Infectious Diseases 1984, 149: 465–470.

    PubMed  Google Scholar 

  9. Magnarelli LA, Meegan JM, Anderson JF, Chappell WA: Comparison of an indirect fluorescent-antibody test with an enzyme-linked immunosorbent assay for serological studies of Lyme disease. Journal of Clinical Microbiology 1984, 20: 181–184.

    PubMed  Google Scholar 

  10. Shresta M, Grodzicki RL, Steere AC: Diagnosing early Lyme disease. American Journal of Medicine 1985, 78: 235–240.

    PubMed  Google Scholar 

  11. Magnarelli LA, Anderson JF: Early detection and persistence of antibodies toBorrelia burgdorferi in persons with Lyme disease. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene (A) 1986, 263: 392–400.

    Google Scholar 

  12. Magnarelli LA, Anderson JF, Johnson RC: Cross-reactivity in serologic tests for Lyme disease and other spirochetal infections. Journal of Infectious Diseases 1987, 156: 183–188.

    PubMed  Google Scholar 

  13. Grodzicki RL, Steere AC: Comparison of immunoblotting and indirect enzyme-linked immunosorbent assay using different antigen preparations for diagnosing early Lyme disease. Journal of Infectious Diseases 1988, 157: 790–797.

    PubMed  Google Scholar 

  14. Coleman JL, Benach JL: Isolation of antigenic components from the Lyme disease spirochete: their role in early diagnosis. Journal of Infectious Diseases 1987, 155: 756–765.

    PubMed  Google Scholar 

  15. Hansen K, Hindersson P, Pedersen NS: Measurements of antibodies toBorrelia burgdorferi flagellum improves serodiagnosis in Lyme disease. Journal of Clinical Microbiology 1988, 26: 338–346.

    PubMed  Google Scholar 

  16. Hansen K, Åsbrink E: Serodiagnosis of erythema migrans and acrodermatitis chronica atrophicans by theBorrelia burgdorferi flagellum enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 1989, 27: 545–551.

    PubMed  Google Scholar 

  17. Waaler E: On the occurrence of a factor in human serum activating the specific agglutination of sheep blood corpuscles. Acta Pathologica et Microbiologica 1940, 17: 172–177.

    Google Scholar 

  18. Burnham TK, Bank PW: Antinuclear antibodies. I. Pattern of nuclear immunofluorescens. Journal of Investigative Dermatology 1974, 62: 526–534.

    PubMed  Google Scholar 

  19. Wassermann A, Neisser A, Bruch C: Eine serodiagnostische Reaktion bei Syphilis. Deutsche Medizinische Wochenschrift 1906, 32: 745–746.

    Google Scholar 

  20. Åsbrink E, Hovmark A, Hederstedt B: The spirochetal etiology of acrodermatitis chronicum atrophicans Herxheimer. Acta Dermatologica et Venereologica 1984, 64: 506–512.

    Google Scholar 

  21. Barbour AG: Isolation and cultivation of Lyme disease spirochete. Yale Journal of Biological Medicine 1984, 57: 521–525.

    Google Scholar 

  22. Magnarelli LA, Anderson JF, Barbour AG: Enzymelinked immunosorbent assays for Lyme disease: reactivity of subunits ofBorrelia burgdorferi. Journal of Infectious Diseases 1989, 159: 43–49.

    PubMed  Google Scholar 

  23. Barbour AG, Heiland RA, Howe TR: Heterogeneity of major proteins in Lyme disease borreliae: a molecular analysis of North American and European isolates. Journal of Infectious Diseases 1985, 152: 478–484.

    PubMed  Google Scholar 

  24. Barbour AG: Immunochemical analysis of Lyme disease spirochetes. Yale Journal of Biological Medicine 1984, 57: 581–586.

    Google Scholar 

  25. Barbour AG, Hayes SF, Heiland RA, Schrumpf ME, Tessier SL: ABorrelia specific monoclonal antibody binds to a flagellar epitope. Infection and Immunity 1986, 52: 549–554.

    PubMed  Google Scholar 

  26. Wilske B, Preac-Mursic V, Schierz G, von Busch K: Immunochemical and immunological analysis of EuropeanBorrelia burgdorferi strains. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene (A) 1986, 263: 92–102.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, University of Umeå, S-901 87, Umeå, Sweden

    S. Bergström

  2. Department of Bacteriology, University of Umeå, S-901 87, Umeå, Sweden

    A. Sjöstedt

  3. Department of Bacteriology, Sahlgrenska Sjukhuset, S-413 45, Gothenburg, Sweden

    L. Dotevall & B. Kaijser

  4. Department of Infectious Disease, Östra Sjukhuset, S-416 85, Gothenburg, Sweden

    L. Dotevall

  5. Symbicom AB, S-901 24, Umeå, Sweden

    B. Ekstrand-Hammarström, C. Wallberg & G. Skogman

  6. Departments of Microbiology and Medicine, University of Texas Health Science Center, 78284, San Antonio, Texas, USA

    A. G. Barbour

Authors
  1. S. Bergström
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Sjöstedt
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. Dotevall
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B. Kaijser
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. B. Ekstrand-Hammarström
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. C. Wallberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. G. Skogman
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. A. G. Barbour
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bergström, S., Sjöstedt, A., Dotevall, L. et al. Diagnosis of Lyme borreliosis by an enzyme immunoassay detecting immunoglobulin G reactive to purifiedBorrelia burgdorferi cell components. Eur. J. Clin. Microbiol. Infect. Dis. 10, 422–427 (1991). https://doi.org/10.1007/BF01968022

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01968022

Keywords

Search

Navigation