Summary
Juvenile hormone (JH) is known to act on the membranes of the follicle cells ofRhodnius, activating a specific Na+, K+-ATPase. This leads to a decrease in volume of the cells and the appearance of spaces between them (patency). The addition of an inhibitor of protein kinase C, 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7), to the medium in vitro inhibits the action of JH on the follicle cells. PDBU (phorbol-12,13-dibutyrate) mimics the action of JH in vitro and the response of the follicle cells to, PDBU is blocked by ouabain. It is concluded that the activation of protein kinase C is a required step in the chain of events leading to activation of the JH-dependent ATPase and set in train by the binding of JH to the membrane.
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Sevala, V.L., Davey, K.G. Action of juvenile hormone on the follicle cells ofRhodnius prolixus: Evidence for a novel regulatory mechanism involving protein kinase C. Experientia 45, 355–356 (1989). https://doi.org/10.1007/BF01957476
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DOI: https://doi.org/10.1007/BF01957476