Abstract
The study of ancient DNA offers the possibility of following genetic change over time. However, the field is plagued by a problem which is unique in molecular biology-the difficulty of verifying results by reproduction. Some of the reasons for this are technical and derive from the low copy number and damaged state of ancient DNA molecules. Other reasons are the unique nature of many of the objects from which DNA is extracted. We describe methodological approaches with which these problems can be alleviated in order to ensure that results are scientific in the sense that they can be reproduced by others.
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References
Asimov, I., The Complete Stories. Doubleday, New York 1992.
Boom, R., Sol, C. J. A., Salimans, M. M. M., Jansen, C. L., Wertheim-van Dillen, P. M. E., and van der Noordaa, J., Rapid and simple method for purification for nucleic acids. J. clin. Microbiol.28 (1990) 495–503.
Cooper, A., Mourer-Chauvire, C., Chambers, G. K., von Haeseler, A., Wilson, A.C., and Pääbo, S., Independent origins of New Zealand moas and kiwis. Proc. natl Acad. Sci. USA89 (1992) 8741–8744.
DeSalle, R., Barcia, M., and Wray, C., PCR jumping in clones of 30-million-year-old DNA fragments from amber preserved termites (Mastotermes electrodominicus). Experientia49 (1993) 906–909.
Golenberg, E. M., Giannasi, D. E., Clegg, M. T., Smiley, C. J., Durbin, M., Henderson, D., and Zurawski, G., Chloroplast DNA sequence from a MioceneMagnolia species. Nature344 (1990) 656–658.
Goloubinoff, P., Pääbo, S., and Wilson, A. C., Evolution of maize inferred from sequence diversity of anAdh2 gene segment from archaeological specimens. Proc. natl Acad. Sci. USA90 (1993) 1997–2001.
Handt, O., Richards, M., Trommsdorff, M., Kilger, C., Simanainen, J., Georgiev, O., Bauer, K., Stone, A., Hedges, R., Schaffner, W., Uttermann, G., Sykes, B., and Pääbo, S., Molecular Genetic Analyses of the Tyrolean Ice Man. Science (1994) in press.
Höss, M., Kohn, M., Knauer, F., Schröder, W., and Pääbo, S., Excrement analysis by PCR. Nature359 (1992) 199.
Höss, M., and Pääbo, S., DNA extraction from Pleistocene bones by a silica-based purification method. Nucleic Acids Res21 (1993) 3913–3914.
Janczewski, D. N., Yuhki, N., Gilbert, D. A., Jefferson, G. T., and O'Brien, S. J., Molecular phylogenetic inference from saber-toothed cat fossils of Rancho La Brea. Proc. natl Acad. Sci. USA89 (1992).
Krajewski, C., Driskell, A. C., Baverstock, P. R., and Braun, M. J., Phylogenetic relationships of the thylacine (Mammalia: Thylacinidae) among dasyuroid marsupials: evidence from cytochromeb DNA sequences. Proc. R. Soc. Lond. B250 (1992) 19–27.
Lawlor, D. A., Dickel, C. D., Hauswirth, W. W., and Parham, P. Ancient HLA genes from 7500-year-old archaeological remains. Nature349 (1991) 785–788.
Lindahl, T., Instability and decay of the primary structure of DNA. Nature362 (1993) 709–715.
Pääbo, S., Ancient DNA: extraction, characterization, molecular cloning and enzymatic amplification. Proc. natl Acad. Sci. USA86 (1989) 1939–1943.
Pääbo, S., Irwin, D. M., and Wilson, A. C., DNA damage promotes jumping between templates during enzymatic amplification. J. biol. Chem.265 (1990) 4718–4721.
Pääbo, S., and Wilson, A. C., Miocene DNA sequences—a dream come true? Curr. Biol.1 (1991) 45–46.
Persson, P., A method to recover DNA from ancient bones. Ancient DNA Newsletter1 (1992) 25–27.
Poinar, H. N., Cano, R. J., and Poinar Jr, G. O., DNA from an extinct plant. Nature363 (1993) 677.
Sykes, B., Ancient DNA: the past comes alive. Nature352 (1991) 381–382.
Thomas, R. H., Schaffner, W., Wilson, A. C., and Pääbo, S., DNA phylogeny of the extinct marsupial wolf. Nature340 (1989) 465–467.
Thomas, W. K., Pääbo, S., Villablanca, F. X., and Wilson, A. C., Spatial and temporal continuity of kangaroo rat populations shown by sequencing mitochondrial DNA from museum specimens. J. molec. Evol.31 (1990) 101–112.
Walsh, P. S., Metzger, D. A., and Higuchi, R., Chelex® 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Bio Techniques10 (1991) 506–513.
Wayne, R. K., and Jenks, S. M., Mitochondrial DNA analysis implying extensive hybridization of the endangered red wolfCanis rufus. Nature351 (1991) 565–568.
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Handt, O., Höss, M., Krings, M. et al. Ancient DNA: Methodological challenges. Experientia 50, 524–529 (1994). https://doi.org/10.1007/BF01921720
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DOI: https://doi.org/10.1007/BF01921720