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Polygenic control of antibody production and correlation with vaccine induced resistance to rabies virus in high and low antibody responder mice

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Summary

The amplification of “high” (H) and “low” (L) multispecific antibody responses achieved respectively by H and L lines of selection GP represents a valuable tool in the genetic study of host-infection interactions. These lines were obtained by bidirectional selective breeding for high (HGP) or low (LGP) antibody production to natural complex antigens. HGP and LGP parental lines and reciprocal F1 hybrids, as well as their F2 segregants and backcrosses were submitted to immunization and challenge with rabies virus CVS strain. Acquired resistance was 1000-fold higher in HGP than LGP mice, with a dominance effect to low antibody production observed in F1 hybrids. An association between high antibody response and acquired resistance (P<0.001) in F2 segrgant mice was noticed. The genetic study was performed in these several populations, with a single dose of 104.5-fold LD50 CVS. We could demonstrate 3 independent loci regulating the anti-rabies antibody production, that are distinct, at least in part, from the 10 genes controlling the antigen selection response (sheep erythrocytes) of selection GP.

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Authors and Affiliations

  1. Laboratório de Imunogenética, São Paulo, Brazil

    M. De Franco, S. Massa & O. A. Sant'Anna

  2. Laboratório de Imunologia Viral of Instituto Butantan, São Paulo, Brazil

    R. C. Vassão

Authors
  1. M. De Franco
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  2. S. Massa
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  3. R. C. Vassão
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  4. M. Siqueira
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  5. O. A. Sant'Anna
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De Franco, M., Massa, S., Vassão, R.C. et al. Polygenic control of antibody production and correlation with vaccine induced resistance to rabies virus in high and low antibody responder mice. Archives of Virology 141, 1397–1406 (1996). https://doi.org/10.1007/BF01718243

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  • DOI: https://doi.org/10.1007/BF01718243

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