Summary
InDactylis glomerata L. subsp.lusitanica, triploid and tetraploid plants were obtained by bilateral sexual polyploidization in crosses between diploid parents known to produce 2n gametes. The polyploid and diploid progeny were compared for allozyme diversity (allele number and heterozygosity), phenological (pollen fertility, inflorescence emergence date), cellular (stomatic cell size) and morphological characters (vegetative biomass, seed weight, total seed number per plant, panicle number and seed number per panicle length) in four contrasting growing conditions. Stomatal cell size and the total number of alleles were significantly lower in diploids than in polyploids which sometimes recovered more than two distinct alleles at a locus via 2n gametes possessing heterozygous loci. Pollen fertility was similar in diploids and tetraploids, but significantly lower in the triploids. Seed weight was significantly higher in polyploids, although the average number of seeds per panicle length and the total number of seeds was higher in the diploid sibs. No phenological differences occurred among ploidy levels whereas such differences were observed inDactylis in all the natural areas of sympatry between diploids and their related polyploids. Our results provide evidence for an instantaneous effect of polyploidization on certain traits and suggest that natural selection would act subsequently on several other traits to allow the establishment of new polyploids.
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References
Berdahl, J.D. & R.E. Barker, 1991. Characterization of autotetraploid Russian wildrye produced with nitrous oxide. Crop Sci. 31:1153–1155.
Bingham, E.T., 1980. Maximizing heterozygosity in autopolyploids. p. 471–489. In: W.H. Lewis (Ed). Polyploidy, Biological Relevance. Plenum Press, New York.
Borrill, M., 1978. Evolution and genetic resources in cocksfoot. Ann. Rep. Welsh Plant Breeding Station, 1977. Aberystwyth: 190–209.
Borrill, M. & R. Linder, 1971. Diploid-tetraploid sympatry inDactylis (Gramineae). New Phytol. 70:1111–1124.
Bretagnolle, F., 1993. Etude de quelques aspects des méchanismes de la polyploïdisation et de ses conséquences évolutives dans le complexe polyploïde du dactyle (Dactylis glomerata L.). Thesis, Univ. Paris XI.
Bretagnolle, F. & J.D. Thompson, 1995. Gametes with somatic chromosome number: their mechanisms of formation and role in the evolution of autopolyploid plants. The New Phytol. 129: 1–22.
Casler, M.D. & P.M. Hugessen, 1988. Performance of tetraploid progeny derived from 2x–4x inter-subspecific crosses inDactylis glomerata L. Genome 30:591–596.
De Haan, A., N.O. Maceira, R. Lumaret & J. Delay, 1992. Production of 2n gametes in diploid subspecies ofDactylis glomerata L. 2. Occurrence and frequency of 2n eggs. Ann. Bot 69:345–350.
Den Nijs, T.P.M. & S.J. Peloquin, 1977. 2n gametes in potato species and their function in sexual polyploidization. Euphytica 26:585–600.
Fagerberg, W.R., T.M. Davis & L.J. Matthews, 1990. Comparison of tetraploid and single gene-induced gigas variants in chickpea (Cicer arietinum). II. Morphological and anatomical leaf characters. Am. J. Bot. 77:300–304.
Falconer, D.S., 1989. Quantitative genetics. Longman Inc. New York.
Felber, F., 1991. Establishment of a tetraploid cytotype in a diploid population: Effect of relative fitness of the cytotypes. J. Evol. Biol. 4:195–207.
Fowler, N. & D.A. Levin, 1984. Ecological constraints on the establishment of a novel polyploid in competition with its diploid progenitor. Am. Nat 124:703–711.
Galbraith, D.W., K. Harkins, J.M. Maddox, N.M. Ayres, D.P. Sharma & E. Firrozabady, 1983. Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220:1049–1051.
Hahn, S.K., K.V. Bai & R. Asiedu, 1990. Tetraploids, triploids, and 2n pollen from diploid interspecific crosses with cassava. Theor. Appl. Genet. 79:433–439.
Harlan, J.R. & J.M. De Wet, 1975. On O. Winge and a prayer: the origin of polyploidy. Bot. Rev. 41:361–390.
Jackson, R.C., 1976. Evolution and systematic significance of polyploidy. Ann. Rev. Ecol. Syst. 7:209–234.
Jongedijk, E., M.S. Ramana, Z. Sawor & J.G.T. Hermsen, 1991a. Formation of first division restitution (FDR) 2n-megaspores through pseudohomotypic division in ds-1 (desynapsis) mutants of diploid potato: routine production of tetraploid progeny from 2xFDR X 2xFDR crosses. Theor. Appl. Genet. 82:645–656.
Jongedijk, E., R.C.B. Hutten, J.M.A. van der Wolk & S.I.J. Schuurmans Stekhoven, 1991b. Synaptic mutants in potato,Solanum tuberosum L. III. Effect of the Sd-1/ds-1 locus (desynapsis) on genetic recombination in male and female meiosis. Genome 34:121–130.
Johnston, S.A., T.P.N. Den Nijs, S.J. Peloquin & J.R. Hanneman. 1980. The significance of genetic balance to endosperm development in interspecific crosses. Theor. Appl. Genet. 57:5–9.
Laroche, J., 1982. Nouvelle technique d'empreintes du matériel végétal. I Etude des surfaces épidermiques et des tissus intemes. Rev. Gén. Bot. 89:173–181.
Levin, D.A., 1975. Minority cytotype exclusion in local plant populations. Taxon 24:35–43.
Lumaret, R., 1988a. Cytology, genetics and evolution in the genusDactylis. CRC Crit. Rev. Plant Sci. 7:55–91.
Lumaret, R., 1988b. Adaptative strategies and ploidy levels. Acta Oecol., Oecol. Plant. 9:83–93.
Lumaret, R., 1991. The diplogamete method and ploidy manipulation in fodder crops breeding. p. 95–100. In: A.P.M. Den Nijs & A. Elgersma (Eds). Fodder Crops Breeding: Achievements, novel strategies and biotechnology. Pudoc, Wageningen.
Lumaret, R. & E. Barrientos, 1990. Phylogenetic relationships and gene flow between sympatric diploid and tetraploid plants ofDactylis glomerata (Gramineae). Plant Syst. Evol. 169:81–96.
Lumaret, R., J.L. Guillerm, J. Delay, A. Ait Lhaj Loufti, J. Izco & M. Jay, 1987. Polyploidy and habitat differentiation inDactylis glomerata L. from Galicia (Spain). Oecologia 73:436–446.
Mc Coy, T.J. & D.E. Rowe, 1986. Single cross alfalfa (Medicago sativa L.) hybrids produced via 2n gametes and somatic chromosome doubling: experimental and theoretical comparisons. Theor. Appl. Genet. 72:80–83.
Maceira, N.O., A.A. De Haan, R. Lumaret, M. Billon & J. Delay, 1992. Production of 2n gametes in diploid subspecies ofDactylis glomerata L. 1. Occurrence and frequency of 2n pollen. Ann. Bot. 69:335–343.
Mayr, E., 1974. Populations, espèces et évolution. Hermann.
Mendiburu, A.O. & S.J. Peloquin, 1977. Bilateral sexual polyploidization in potatoes. Euphytica 26:573–583.
Mendiburu, A.O. & S.J. Peloquin, 1979. Gene-centromere mapping by 4x-2x matings in potatoes. Theor. Appl. Genet. 54:177–180.
Parrott, W.A., R.R. Smith & M.M. Smith, 1985. Bilateral sexual tetraploidization in red clover. Canadian J. Genet. Cytol. 27:64–68.
Peloquin, S.J., G.L. Yerk, J.E. Werner & E. Darno, 1989. Potato breeding with haploids and 2n gametes. Genome 32:1052–1059.
Pfeiffer, T.W. & T. Bingham, 1984. Comparisons of alfalfa somaclonal and sexual derivatives from the same genetic source. Theor. Appl. Genet. 67:263–266.
Pundir, R.P.S., N.K. Rao & L.J.G. van der Maesen, 1983. Induced tetraploidy in chickpea (Cicer arietinum L.). Theor. Appl. Genet. 65:119–122.
Ray, M. & M.K. Tokach, 1992. Cytology of 2n pollen formation in diploid crested wheat grass,Agropyron cristatum. Crop Sci. 32:1361–1365.
Rice, W.R., 1989. Analysing tables of statistical tests. Evolution 43:223–225.
Sala, C.A., E.L. Camadro, M.T. Salabury & A.O. Mendiburu, 1989. Cytological mechanism of 2n pollen formation and unilateral sexual polyploidization inLolium. Euphytica 43:1–6.
S.A.S. Institute Inc., 1989. SAS user's guide: Statistics version 5. Cary, North Carolina.
Sgorbati, S., S.M. Masci, V. Soler & M. Marchi, 1989. Rapid cytofluorimetric determination of leaf nuclear DNA content in the polyploid seriesRanunculus narsicus (R. auricomus agg., Ranunculaceae). Pl. Syst. Evol. 167:219–226.
Sokal, R.R. & F.J. Rohlf, 1981. Biometry. Freeman, New York.
Stebbins, G.L., 1985. Polyploidy, hybridisation and the invasion of new habitats. Ann. Missouri Bot. Gard. 72:824–832.
Stebbins, G.L. & D. Zohary, 1959. Cytogenetic and evolutionary studies in the genusDactylis. I. Morphology, distribution and interrelationships of the diploid subspecies. Univ. Calif. Berkeley Publ. Bot. 31:1–40.
Tal, M., 1980. Physiology of polyploids. p. 61–75. In: W.H. Lewis (Ed). Polyploidy, Biological Relevance. Plenum Press, New York.
Tan, G.Y. & G.M. Dunn, 1973. Relationship of stomatal length and frequency and pollen-grain diameter to ploidy level inBromus inermis Lyess. Crop. Sci. 13:332–334.
Tomekpe, K. & R. Lumaret, 1991. Association between quantitative traits and allozyme heterozygosity in a tetrasomic species:Dactylis glomerata L. Evolution 45:350–370.
van Dijk, P.V. & W. van Delden, 1990. Evidence for autotetraploidy inPlantago media and comparisons between natural and artificial cytotypes concerning cell size and fertility. Heredity 65:344–357.
van Dijk, P.V., M. Hartog & W. van Delden, 1992. Single cytotype areas in autopolyploidPlantago media L. Biol. J. Linn. Soc. 46:315–332.
van Santen, E., P.M. Hugessen & M.D. Casler, 1991. Identification and frequency of tetraploid progeny from 2x-4x and 4x-2x crosses inDactylis. Genome 34:273–278.
Veilleux, R., 1985. Diploid and polyploid gametes in crop plants: mechanisms of formation and utilization in plant breeding. Plant Breed. Rev. 3:252–288.
Werner, J.E. & S.J. Peloquin, 1991a. Occurrence and mechanisms of 2n egg formation in 2x potato. Genome 34:975–982.
Werner, J.E. & S.J. Peloquin, 1991b. Significance of allelic diversity and 2n gametes for approaching maximum heterozygosity in 4x potatoes. Euphytica 58:21–29.
Winner, B.J., D.R. Brown & K.M. Michels, 1991. Statistical principles in experimental design. Mc Graw-Hill, Inc.
Yen, Y. & G. Kimber, 1990. Meiotic behaviour of induced autotetraploids inTriticum L. Genome 33:302–307.
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Bretagnolle, F., Lumaret, R. Bilateral polyploidization inDactylis glomerata L. subsp.lusitanica: occurrence, morphological and genetic characteristics of first polyploids. Euphytica 84, 197–207 (1995). https://doi.org/10.1007/BF01681812
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DOI: https://doi.org/10.1007/BF01681812