Abstract
The method of Computer modeling was used to build the three-dimensional structure of Angiogenin, based on its homology to bovine pancreatic ribonuclease A, the X-ray crystal structure of which is known. The positions of the catalytically important residues His-13, Lys-40, and His-114 were considered. The fit of the backbone of angiogenin to that of bovine pancreatic ribonuclease A (bpr) was then optimized by taking account of residue deletions. The X-ray structure of bovine pancreatic ribonuclease A with the bound inhibitor, (3′–5′)Cytidinephosphorylguanosine (CpG) was used as a template to fit the active site of angiogenin. Finally the model of angiogenin was energy minimized under a single layer of explicit water molecules representing the first solvation shell.
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Chen, J., Howlin, B., Tomkinson, N.P. et al. Construction of a preliminary three-dimensional structure of angiogenin. J Chem Crystallogr 24, 27–35 (1994). https://doi.org/10.1007/BF01665343
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DOI: https://doi.org/10.1007/BF01665343