Abstract
In this study we have demonstrated the presence of permeability factors (PF) in the extracellular products (ECP) of 16 strains of the bacterial fish pathogenYersinia ruckeri. The production and detection of these factors were dependent on the growth media and the ECP extraction procedure. The best results were obtained with a cellophane plate method and use of brain-heart infusion agar (BHIA) medium. The loss of necrotizing activity after heating the samples at 80°C for 15 min indicates the thermolabile nature of the toxin. In addition, this activity was not correlated with the production of hemolysins and cytotoxins by theY. ruckeri strains.
Similar content being viewed by others
Literature Cited
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Cruz JA de la, Rodriguez A, Tejedor C, de Lucas E, Orozco LR (1986) Isolation and identification ofYersinia ruckeri, causal agent of enteric redmouth disease (ERM), for the first time in Spain. Bull Eur Ass Fish Pathol 6:43–44
Furhmann H, Bohm KH, Schlotfeldt HJ (1983) An outbreak of enteric redmouth disease in West Germany. J Fish Dis 6:309–311
Krovacek K, Faris A, Mansson I (1987) Cytotoxic and skin permeability factors produced byVibrio anguillarum. Aquaculture 67:87–91
Lallier R, Bernard F, Lalonde G (1984) Difference in the extracellular products of two strains ofAeromonas hydrophila virulent and weakly virulent for fish. Can J Microbiol 30:900–904
Lesel R, Lesel M, Gavini F, Villaume A (1983) Outbreak of enteric redmouth disease in rainbow trout,Salmo gairdneri Richardson, in France. J Fish Dis 6:385–387
Liu PV (1957) Survey of hemolysin production among species of pseudomonas. J Bacteriol 74:718–727
Llewellyn LC (1980) A bacterium with similarities to the redmouth andSerratia liquefaciens (Grimes and Hennerty) causing mortalities in hatchery reared salmonids in Australia. J Fish Dis 3:29–39
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory
Olivier G, Lallier R, Lariviere S (1981) A toxigenic profile ofAeromonas hydrophila andAeromonas sobria isolated from fish. Can J Microbiol 27:330–333
Romalde JL, Barja JL, Toranzo AE (1990) Estudio de las actividades enzimáticas y toxigénicas de los productos extracelulares deYersinia ruckeri. In: Landín A, Cerviño A (eds) Actas del III Congreso Nacional de Acuicultura. Santiago de Compostela, pp 767–772
Romalde JL, Lemos ML, Conchas RF, Bandín I, Toranzo AE (1990) Adhesive properties and other virulence factors inYersinia ruckeri. In: Perkins FO, Cheng TC (eds) Pathology in marine science. New York: Academic Press, pp 123–132
Romalde JL, Conchas RF, Toranzo AE (1991) Evidence thatYersinia ruckeri possesses a high affinity iron uptake system. FEMS Microbiol Lett 80:121–126
Rucker RR (1966) Redmouth disease in rainbow trout (Salmo gairdneri). Bull Off Int Epizoot 65:825–830
Santos Y, Lallier R, Bandín I, Lamas J, Toranzo AE (1991) Susceptibility of turbot (Scophthalmus maximus), coho salmon (Oncorhynchus kisutch), and rainbow trout (O. mykiss) to strains ofVibrio anguillarum and their exotoxins. J Appl Ichthyol 7:160–167
Stevenson RMW, Daly JG (1982) Biochemical and serological characteristics of Ontario isolates ofYersinia ruckeri. Can J Fish Aqua Sci 39:870–876
Ullah A, Arai T (1983) Pathological activities of the naturally occurring strains ofEdwarsiella tarda. Fish Pathol 18:65–70
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Romalde, J.L., Santos, Y. & Toranzo, A.E. Presence of skin permeability factors in the extracellular products ofYersinia ruckeri . Current Microbiology 24, 263–267 (1992). https://doi.org/10.1007/BF01577330
Issue Date:
DOI: https://doi.org/10.1007/BF01577330