Abstract
The NADP+-specific glutamate dehydrogenase inEscherichia coli K12 has been shown to be phosphorylated in vivo when the cells are grown in a low-phosphate minimal salts medium containing32P inorganic phosphate. The amount of radioactivity incorporated into the enzyme is different depending on the growth phase of the culture, with the highest level of32P incorporation occurring during the mid-exponential growth phase. Previously reported studies have demonstrated that the enzyme is also phosphorylated in vitro in an ATP-dependent reaction.
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Literature Cited
Brenchley JE, Magasanik B (1973) Regulation of the synthesis of enzymes responsible for glutamate formation inKlebsiella aerogenes. J Biol Chem 248:6122–6128
Cortay JC, Rieul LC, Duclos B, Cozzone AJ (1986) Characterization of the phosphorproteins ofEscherichia coli cells by electrophoretic analysis. Eur J Biochem 159: 227–237
Garnak M, Reeves HC (1979) Purification and properties of phosphorylated isocitrate dehydrogenase ofEscherichia coli. J Biol Chem 254:7915–7920
Halpern YS, Umbarger HE (1961) Utilization ofl-glutamic and 2-oxoglutaric acid as sole sources of carbon byEscherichia coli. J Gen Microbiol 26:175–183
Kinghorn JR, Pateman JA (1973) NAD and NADPl-glutamate dehydrogenase activity and ammonium regulation inAspergillus nidulans. J Gen Microbiol 78: 39–46
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Lin H-PP, Reeves HC (1991a) Phosphorylation ofEscherichia coli NADP+-specific glutamate dehydrogenase. Curr Microbiol 22:181–184
Lin H-PP, Reeves HC (1991b) Purification and characterization of NADP+-specific glutamate dehydrogenase fromEscherichia coli. Curr Microbiol 22:371–376
Magasanik B (1982) Genetic control of nitrogen assimilation in bacteria. Annu Rev Genet 16:135
Reitzer LJ, Magasanik B (1987) Ammonium assimilation and the biosynthesis of glutamine, glutamate, aspartate, asparagine,l-alanine, andd-alanine. In:Neidhardt FC, Ingraham JL, Low KB, Magasanik B, Schaechter M, Umbarger HE (eds)Echerichia coli andSalmonella typhimurium: cellular and molecular biology. Washington DC: American Society for Microbilogy, pp 302–320
Robinson SA, Slade AP, Fox GG, Philips R, Ratcliffe RG, Stewart GR (1991) The role of glutamate dehydrogenase in plant nitrogen metabolism. Plant Physiol 95:509–516
Robinson SA, Stewart GR, Phillips R (1992) Regulation of glutamate dehydrogenase activity in relation to carbon limitation and protein catabolism in carrot cell suspension cultures. Plant Physiol 98:1190–1195
Stock JB, Alexander JN, Stock AM (1989) Protein phosphoryllation and regulation of adaptive responses in bacteria. Microbiol Rev 53:450–490
Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354
Tyler B (1978) Regulation of the assimilation of nitrogen compounds. Annu Rev Biochem 47:1127–1162
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Lin, HP.P., Reeves, H.C. In vivo phosphorylation of NADP+ glutamate dehydrogenase inEscherichia coli . Current Microbiology 28, 63–65 (1994). https://doi.org/10.1007/BF01569047
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DOI: https://doi.org/10.1007/BF01569047