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The possession of coli surface antigen CS6 by enterotoxigenicEscherichia coli of serogroups O25, O27, O148, and O159: a possible colonization factor?

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Abstract

A total of 134 enterotoxigenicEscherichia coli (ETEC) of serogroups O25, O27, O148, and O159 were tested in the enzyme-linked immunosorbent assays for the colonization factor antigens I (CFA/I), CFA/II (coli surface antigens CS1, 2 and 3) and putative colonization factor (PCF) 8775 (CS4, 5 and 6). CS6 was detected without CS4 or CS5 in 94% of the strains of serogroup O25, 86% of strains of serogroup O27, 87% of strains of serogroup O148, and 29% of strains of serogroup, O159. The frequency with which CS6 occurs in ETEC of common serotypes without the antigens CS4 or CS5 suggests that it might be a colonization factor.

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Literature Cited

  1. Cravioto A, Scotland SM, Rowe B (1982) Haemagglutination activity and colonization factor antigens I and II in enterotoxigenic and non-enterotoxigenic strains ofEscherichia coli isolated from humans. Infect Immun 36: 189–197

    PubMed  Google Scholar 

  2. Dupont HL, Formal SB, Hornick RB, Snyder MJ, Libonati JP, Sheahan DG, LaBrec EH, Kalas JP (1971) Pathogenesis ofEscherichia coli diarrhea. N Engl J Med 285: 1–9

    PubMed  Google Scholar 

  3. Echeverria P, Ørskov F, Ørskov I, Pliantrangchang D (1982) Serotypes of enterotoxigenicEscherichia coli in Thailand and the Philippines. Infect Immun 36: 851–856

    PubMed  Google Scholar 

  4. Evans DG, Evans DJ (1978) New surface-associated heatlabile colonization factor antigen (CFA/II) produced by enterotoxigenicEscherichia coli of serogroups O6 and O8. Infect Immun 21: 638–647

    PubMed  Google Scholar 

  5. Evans DG, Evans DJ, Tjoa WS, DuPont HL (1978) Detection and characterisation of colonization factors of enterotoxigenicEscherichia coli isolated from adults with diarrhea. Infect Immun 19: 727–736

    PubMed  Google Scholar 

  6. Gross RJ, Rowe B, Henderson A, Byatt ME, McLaurin JC (1976) A newEscherichia coli O-group, O159, associated with outbreaks of enteritis in infants. Scand J Infect Dis 8: 195–198

    PubMed  Google Scholar 

  7. Hobbs BC, Rowe B, Kendall M, Turnbull PCB, Ghosh AC (1976)Escherichia coli 027 in adult diarrhoea. J Hyg Camb 77: 393–400

    PubMed  Google Scholar 

  8. Honda T, Arita M, Miwatani T (1984) Characterisation of new hydrophobic pili of human enterotoxigenicEscherichia coli: a possible new colonization factor. Infect Immun 43: 959–965

    PubMed  Google Scholar 

  9. Kudoh Y, Zen-Yoji H, Matsushita S, Sakai S, Maruyama T (1977) Outbreaks of acute enteritis due to heat-stable enterotoxin-producing strains ofEscherichia coli. Microbiol Immunol 21: 175–178

    PubMed  Google Scholar 

  10. Levine MM, Nalin DR, Hoover DL, Bergquist EJ, Hornick RB, Young CR (1979) Immunity to enterotoxigenicEscherichia coli. Infect Immun 23: 729–736

    PubMed  Google Scholar 

  11. Levine MM, Ristaino P, Marley G, Smyth C, Knutton S, Boedeker E, Black R, Young C, Clements ML, Cheney C, Patnaik R (1984) Coli surface antigens 1 (CS1) and 3 (CS3) of colonization factor antigen II positive enterotoxigenicEscherichia coli: morphology, purification and immune responses in man. Infect Immun 44: 409–420

    PubMed  Google Scholar 

  12. MacDonald KL, Eidson M, Strohmeyer C, Levy ME, Wells JG, Puhr ND, Wachsmuth K, Hargrett NT, Cohen ML (1985) A multistate outbreak of gastrointestinal illness caused by enterotoxigenicEscherichia coli in imported semisoft cheese. J Infect Dis 151: 716–720

    PubMed  Google Scholar 

  13. McConnell MM, Thomas LV, Day NP, Rowe B (1985) Enzyme-linked immunosorbent assays for the detection of adhesion factor antigens of enterotoxigenicEscherichia coli. J Infect Dis 152: 1120–1127

    PubMed  Google Scholar 

  14. Merson MH, Ørskov F, Ørskov I, Sack RB, Huq I, Koster FT (1979) Relationship between enterotoxin production and serotype inEscherichia coli. Infect Immun 23: 325–329

    PubMed  Google Scholar 

  15. Merson MH, Black RE, Khan MU, Huq I (1980) Epidemiology of cholera and enterotoxigenicEscherichia coli diarrhoea. In: Ouchterlony O, Holmgren J (eds) Cholera and related diseases: 43rd Nobel symposium, Stockholm. Basel: S Karger, pp 34–45

    Google Scholar 

  16. Morris GK, Merson MH, Sack DA, Wells JG, Martin WT, DeWitt WE, Feeley JC, Sack RB, Bessudo DM (1976) Laboratory investigation of diarrhea in travelers to Mexico: an evaluation of methods for detecting enterotoxigenicE. coli. J Clin Microbiol 3: 486–495

    PubMed  Google Scholar 

  17. Ørskov F, Ørskov I, Evans DJ, Sack RB, Sack DA, Wadstrom T (1976) SpecialEscherichia coli serotypes among enterotoxigenic strains from diarrhoea in adults and children. Med Microbiol Immunol 162: 73–80

    PubMed  Google Scholar 

  18. Reis MHL, Matos DP, Pestana de Castro AF, Toledo MRF, Trabulsi LR (1980) Relationship among enterotoxigenic phenotype, serotypes, and sources of strains in enterotoxigenicEscherichia coli. Infect Immun 28: 24–27

    PubMed  Google Scholar 

  19. Riordan T, Gross RJ, Rowe B, Scotland SM, Johnston SM (1985) An outbreak of food-borne enterotoxigenicEscherichia coli diarrhoea in England. J Infect 11: 167–171

    PubMed  Google Scholar 

  20. Rowe B, Taylor J, Bettelheim KA (1970) An investigation of traveller's diarrhoea. Lancet 1: 1–5

    PubMed  Google Scholar 

  21. Rowe B, Gross R, Takeda Y (1983) Serotyping of enterotoxigenicEscherichia coli isolated from diarrhoeal travellers from various Asian countries. FEMS Microbiol Lett 20: 187–189

    Google Scholar 

  22. Sack RB (1975) Human diarrheal disease caused by enterotoxigenicEscherichia coli. Annu Rev Microbiol 29: 333–353

    PubMed  Google Scholar 

  23. Scotland SM, Richmond JE, Rowe B (1983) Adhesion of enteropathogenic strains ofEscherichia coli (EPEC) to HEp-2 cells is not dependent on the presence of fimbriae. FEMS Microbiol Lett. 20: 191–195

    Google Scholar 

  24. Smyth CJ (1982) Two mannose-resistant haemagglutinins of enterotoxigenicEscherichia coli of serotype O6:K15-H16 or H− isolated from travellers and infantile diarrhoea. J Gen Microbiol 128: 2081–2096

    PubMed  Google Scholar 

  25. Thomas LV, Rowe B (1982) The occurrence of colonisation factors (CFA/I, CFA/II and E8775) in enterotoxigenicEscherichia coli from various countries in South East Asia. Med Microbiol Immunol 171: 85–90

    PubMed  Google Scholar 

  26. Thomas LV, Rowe B (1982) An investigation of the adhesion of enterotoxigenicEscherichia coli to human intestinal epithelial cells in vitro. Soc Gen Microbiol Q 9: 7

    Google Scholar 

  27. Thomas LV, McConnell MM, Rowe B, Field AM (1985) The possession of three novel coli surface antigens by enterotoxigenicEscherichia coli strains positive for the putative colonisation factor PCF8775. J Gen Microbiol 131: 2319–2326

    PubMed  Google Scholar 

  28. Van den Bosch J, Verboom Sohmer U, Postma P, De Graaf J, McLaren DM (1980) Mannose-senstive and mannose-resistant adherence to human uroepithelial cells and urinary virulence ofEscherichia coli. Infect Immun 29: 226–233

    PubMed  Google Scholar 

  29. Wachsmuth K, Wells J, Shipley P, Ryder R (1979) Heatlabile enterotoxin production in isolates from a shipboard outbreak of human diarrheal illness. Infect Immun 24: 793–797

    PubMed  Google Scholar 

  30. Wadstrom T, Faris A, Ljungh A, Habte D (1980) Attachment of enterotoxigenicEscherichia coli with CFA/I and CFA/II to human intestinal cells. In: 15th US-Japan conference on cholera. United States Department of Health, Education and Welfare, Public Health Service publication NIH 80-2003, pp 459–470

  31. Wood LV, Wolfe WH, Ruiz-Palacios G, Foshee WS, Corman LI, McCleskey F, Wright JA, DuPont HL (1983) An outbreak of gastroenteritis due to a heat-labile enterotoxin-producing strain ofEscherichia coli. Infect Immun 41: 931–934

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Division of Enteric Pathogens, Central Public Health Laboratory, Colindale Avenue, NW9 5HT, London, UK

    Moyra M. McConnell, Linda V. Thomas, Sylvia M. Scotland & Bernard Rowe

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  1. Moyra M. McConnell
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  2. Linda V. Thomas
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  3. Sylvia M. Scotland
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  4. Bernard Rowe
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McConnell, M.M., Thomas, L.V., Scotland, S.M. et al. The possession of coli surface antigen CS6 by enterotoxigenicEscherichia coli of serogroups O25, O27, O148, and O159: a possible colonization factor?. Current Microbiology 14, 51–54 (1986). https://doi.org/10.1007/BF01568102

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