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Comparison of maize root mucilages isolated from root exudates and root surface extracts by complementary cytological and biochemical investigations

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Summary

A strategy to obtain fractions enriched in mucilages secreted by root caps or produced by the rhizodermis of axenicallygrown maize seedlings is proposed. It involves a two-step procedure allowing the successive collection of root exudates and surface extracts from the same set of intact, sterile maize plants. Cytological controls were performed at each phase of collection. Whereas root cap mucilage is easily collected in water after one day's extraction, under conditions favouring secretory activity, rhizodermal mucilage remains tightly adherent to the root surface. It can be better extracted using neutral saline buffer assisted by gentle shaking at low temperature. Acidic saline buffer is unsuitable as it induces cell lysis and release of cell wall components.

Biochemical analyses confirm that fractions enriched in root cap mucilage contain very high levels of fucose and galactose, high levels of arabinose, xylose and glucose and trace amounts of mannose. Fractions enriched in rhizodermal mucilage contain large amounts of glucose, moderate amounts of arabinose, xylose, mannose and galactose and trace levels of fucose. Isoelectric focusing and SDS-PAGE indicate that there are numerous similarities in the protein composition of materials enriched in root cap mucilages from root exudates or aqueous root surface extracts. However, specific protein bands that could be characteristic of rhizodermal mucilage are obtained using neutral saline buffer extracts. According to these biochemical data, the two-step procedure used in the present study appears to be useful for further biochemical characterization of both types of mucilages.

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Abbreviations

BSA:

bovine serum albumin

BSTFA:

N,O-bis (trimethylsilyl)-trifluoroacetamide

DTT:

dithiothreitol

i. d.:

internal diameter

MW:

molecular weight

PATAg:

periodic acid-thiosemicarbazide-silver proteinate

PVPP:

polyvinylpolypyrrolidone

RE:

root exudates

RSE:

root surface extracts

TMCS:

trimethylchlorosilane

TMS:

trimethylsilyl

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Authors and Affiliations

  1. Laboratoire de Reconnaissance Cellulaire et Amélioration des Plantes, Université Lyon I, Bât. 741, Lyon

    Annie Chaboud & Mireille Rougier

  2. Centre de Microscopic Electronique Appliqué à la Biologie et la Géologie, Université Lyon I, Lyon

    Annie Chaboud & Mireille Rougier

Authors
  1. Annie Chaboud
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  2. Mireille Rougier
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Chaboud, A., Rougier, M. Comparison of maize root mucilages isolated from root exudates and root surface extracts by complementary cytological and biochemical investigations. Protoplasma 156, 163–173 (1990). https://doi.org/10.1007/BF01560654

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  • DOI: https://doi.org/10.1007/BF01560654

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