Summary
The absolute requirement of oxygen for a potato tuber (cv. Pentland Crown) to display a resistant response toErwinia carotovora has been demonstrated in EPR measurements. These show that a relatively stable free radical is formed in inoculated hosts only after exposure to air. In an attempt to isolate and identify unstable free radical precursors, experiments have been conducted in which the chemical spin trap α-(4-pyridyl 1-oxide)-N-tert-butyl-nitrone (POBN) was incorporated into the tissue either at the time of inoculation or upon exposure to air. A single radical adduct was observed with spectral parameters that resemble those of a lipid-derived radical.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bowler C, van Montagu M, Inze D (1992) Superoxide dismutase and stress tolerance. Annu Rev Plant Physiol Plant Mol Biol 43: 83–116
Buettner GR (1987) Spin trapping: ESR parameters of spin adducts. Free Radicals Biol Med 3: 259–303
Connor HD, Fischer V, Mason RP (1986) A search for oxygen-centred free radicals in the lipoxygenase/linoleic acid system. Biochem Biophys Res Comm 141: 614–621
Das DK, Essman WB (eds) (1990) Oxygen radicals: systemic events and disease processes. Karger, Basel
Doke N (1983) Involvement of Superoxide anion generation in the hypersensitive response of potato tuber tissues to infection with an incompatible race ofPhytophthora infestans and to the hyphal wall components. Physiol Plant Pathol 23: 345–357
Elstner EF (1982) Oxygen activation and oxygen toxicity. Annu Rev Plant Physiol 33: 73–96
Gestetner B, Conn EE (1974) The 2-hydroxylation of transcinnamic acid by chloroplasts fromMelilotus alba. Arch Biochem Biophys 163: 617–624
Klebanoff SJ (1980) Oxygen metabolism and the toxic properties of phagocytes. Ann Int Med 93: 480–489
Lyon GD (1989) The biochemical basis of resistance of potatoes to soft rotErwinia spp. — a review. Plant Pathol 38: 313–339
Moerschbacher BM, Noll UM, Flott BE, Reisner HJ (1988) Lignin biosynthetic enzymes in stem rust infected resistant and susceptible near-isogenic wheat lines. Physiol Mol Plant Pathol 33: 33–46
Monk LS, McPhail DB, Goodman BA, Davies HV (1989) An electron spin resonance investigation of internal rust spot, a physiological disorder of the potato tuber. Free Radicals Res Comm 5: 345–350
Peters JW, Foote CS (1976) Chemistry of Superoxide ion. II. Reaction with hydroperoxides. J Am Chem Soc 98: 873–875
Rosen GM, Rauckman EJ (1981) The spin trapping of biologically generated free radicals. In: Rodgers MAJ, Powers EL (eds) Oxygen and oxyradicals in chemistry and biology. Academic, London, pp 97–108
Symons MCR (1978) Chemical and biological aspects of electron spin resonance spectroscopy. Van Norstrand Reinhold, London
Sutherland MW (1991) The generation of oxygen radicals during host plant responses to infection. Physiol Mol Plant Pathol 39: 79–93
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Deighton, N., Johnston, D.J., Glidewell, S.M. et al. The involvement of oxygen-derived free radicals in the resistant response of potato tubers toErwinia carotovora . Protoplasma 171, 167–169 (1992). https://doi.org/10.1007/BF01403731
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01403731