Summary
Plants of bean (Phaseolus vulgaris) inoculated first on one primary leaf with strain NY15 of bean common mosaic virus, as inducer, and after three days, on the opposite leaf, with the strain NL3 of bean black root virus, as challenger, did not show systemic necrosis characteristic of the latter strain. This interference phenomenon was studied by determining the amount, distribution and localization of both strains in the part of stem between primary leaves and first trifoliolate leaf in both challenge-inoculated and singly inoculated (control) plants. In dot-blot immunoassay, NL3 was detected seven days after its inoculation as challenger, whereas in control plants its presence was established on day four. Immunostained thick sections revealed a large accumulation of NL3 antigen on day eight in both phloem and cambium, but not yet in the xylem and cortex, contrasted with the controls. In immunogoldsilver stained semi-thin sections, most of the NL3 label was present in the companion cells and other phloem parenchyma cells, while in the control plants this virus was also present in xylem vessels and xylem parenchyma cells. Inducer strain NY15 was abundantly present in practically all the cells, including xylem vessels, from day two after challenge inoculation onwards. It is concluded that inducer strain NY15 hampers transport of NL3 to, and its spread in, the stem and prevents the latter strain from exerting its deleterious influence on the water conducting elements.
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References
Drijfhout E (1978) Genetic interaction betweenPhaseolus vulgaris and bean common mosaic virus with implications for strain identification and breeding for resistance. Agricultural Research Reports 872, PUDOC, Wageningen
Hibi T, Saito Y (1985) A dot-blot immunobinding assay for the detection of tobacco mosaic virus in infected tissue. J Gen Virol 66: 1191–1194
Khan JA, Lohuis H, Goldbach RW, Dijkstra J (1990) Distinction of strains of bean common mosaic virus and blackeye cowpea mosaic virus using antibodies to N- and C-or N-terminal peptide domains of coat protein. Ann Appl Biol 117: 583–593
Khan JA, Lohuis H, Bakardjieva N, Peters D, Goldbach RW, Dijkstra J (1994) Interference between two strains of bean common mosaic virus is accompanied by suppression of symptoms without affecting replication of the challenging virus. J Phytopathol (in press)
Khan JA, Lohuis H, Goldbach RW, Dijkstra J (1993) Sequence data to settle the taxonomic position of bean common mosaic virus and blackeye cowpea mosaic virus isolates. J Gen Virol 74: 2243–2249
Lent JWM van, Verduin BJM (1987) Detection of viral antigen in semi-thin sections of plant tissue by immunogold-silver staining and light microscopy. Neth J Plant Pathol 93: 261–272
McKern NM, Mink GI, Barnett OW, Mishra A, Whittaker LA, Silbernagel MJ, Ward CW, Shukla DD (1992a) Isolates of bean common mosaic virus comprising two distinct potyviruses. Phytopathology 82: 923–929
McKern NM, Ward CW, Shukla DD (1992b) Strains of bean common mosaic virus consist of at least two distinct potyviruses. In: Barnett OW (ed) Potyvirus taxonomy. Springer, Wien New York, pp 407–414 (Arch Virol (Suppl] 5)
Vetten HJ, Lesemann D-E, Maiss E (1992) Serotype A and B strains of bean common mosaic virus are two distinct potyviruses. In: Barnett OW (ed) Potyvirus taxonomy. Springer, Wien New York, pp 415–431 (Arch Virol [Suppl] 5)
Wang W-Y (1983) Serology of bean common mosaic virus strains. M.Sc. Thesis, Washington State University, Pullman, Washington
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Khan, J.A., Lohuis, H., Goldbach, R.W. et al. Distribution and localization of bean common mosaic virus and bean black root virus in stems of doubly infected bean plants. Archives of Virology 138, 95–104 (1994). https://doi.org/10.1007/BF01310041
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DOI: https://doi.org/10.1007/BF01310041