Abstract
In the present investigation, suitability of nuclear magnetic resonance (NMR) spectrometry for total stool fat quantification in patients with normal or impaired exocrine pancreatic function (chronic pancreatitis) has been analyzed in comparison with a conventional chloroform-methanol extraction technique. Basic temperature-dependence studies of NMR spectrometry (90°/180° radiofrequency pulse sequence) on 21 chloroform-methanol extracted pure total stool lipid standards (weight range: 0.05–1.6 g) revealed significantly (P<0.05) improving correlations between NMR signal amplitudes and corresponding weights at increasing temperatures (r=0.952/40° C,r=0.965/60° C,r=0.988/80° C), thus indicating 80° C as optimal temperature for NMR spectrometric total stool fat quantification. In subsequent comparative measureemnts of lyophilized stool samples, NMR spectrometry (at 80° C) and conventional chloroform-methanol extraction provided significantly (P<0.001) correlated results with respect to total fecal fat contents/day of quantitatively collected and homogenized stools in 93 patients with known exocrine pancreatic function (secretin-pancreozymin test), irrespective of whether correlations were determined for all 93 patients (r=0.983) or separately for patients with normal (N=45;r=0.867), moderately reduced (N=31;r=0.946), or highly reduced (N=17;r=0.992) exocrine pancreatic function and correspondingly increased total fecal fat excretions.
Similar content being viewed by others
References
Van de Kamer JH, Ten Bokkel Huinink H, Weyers HA: Rapid method for the determination of fat in the feces. J Biol Chem 177:347–355, 1949
Bowers MA, Lund PK, Mathies JC: A rapid, reliable procedure for the determination of total fecal lipids, with observation of the lipids excreted by human subjects in normal and pathological states. Clin Chim Acta 9:344–347, 1964
Kuchel PW:In Critical Reviews in Analytical Chemistry. B Campbell, L Waites (eds). Boca Raton, Florida, CRC Press, 1981, pp 155–231
Farrar TC, Becker ED: Pulse and Fourier Transform NMR. New York, Academic Press, 1971
Casey JC, Miles CA: Determination of the fat content of meat using nuclear magnetic resonance. J Sci Food Agric 25:1151–1161, 1974
Nilsson R, Kolar K: Rapid physical methods for the determination of fat content in meat products. Livsmedelsteknik 16:258–262, 1974
Moisio T, Timonen E, Krevla M: A rapid method for the determination of the dry matter and fat content of cheese and processed cheese. Milchwirtschaft 27:73–75, 1972
Kern P, Sieber R, Rüst P: Versuche zur Bestimmung der Fett- und Wassergehalte in Käse unter Verwendung eines Kernresonanzgerätes für die Fettbestimmung. Schweiz Milchwirtsch Forsch 3:6–8, 1974
Chapman D, Richards RE, Yorke RW: A nuclear magnetic resonance study of the liquid/solid content of margarine fat. J Am Oil Soc 37:243–246, 1960
Suzuki T, Kanno O, Toyama H: Determination of the solid fat and water content in margarine by means of highresolution NMR. J Jpn Oil Chem Soc 19:1019–1024, 1970
Tympner F, Domschke S, Domschke W, Classen M, Demling L: Reproducibility of the response to secretin and secretin plus pancreozymin in man. Scand J Gastroenterol 9:377–381, 1974
Troll U, Berg G: Zur Methodik der Stuhlfettbestimmung. Med Ernaehr 9:172–174, 1968
Snedecor GW, Cochran WG: Statistical Methods, 6th ed. Ames, Iowa, Iowa State University Press, 1967
Fukushima E, Roeder SBW:In Experimental Pulse NMR. Reading, Massachusetts, Addison-Wesley, 1981, p 126
Kelleher J, Walker BE, Losowsky MS: An assessment of the Van de Kamer method for estimation of fecal fat. Clin Chim Acta 30:267–271, 1970
Pimparkar BD, Tulsky EG, Kalser MH, Bockus HL: Correlation of radioactive and chemical fecal fat determinations in various malabsorption syndromes. II. Results in idiopathic steatorrhea and diseases of the pancreas. Am J Med 30:927–939, 1961
Thompson JB, Su CK, Ringrose RE, Welsh JD: Fecal triglycerides. II. Digestive vs absorptive steatorrhea. J Lab Clin Med 73:521–530, 1969
Bo-Linn GW, Fordtran JS: Fecal fat concentration in patients with steatorrhea. Gastroenterology 87:319–322, 1984
Skala I, Krondl A, Vulterinova M, Stastna R, Vavrinkova H, Horackova J, Parikova V: Composition of feces in steatorrhea of different etiology. Mutual relationship between the volume of feces, water, dry matter, nitrogen and fat content. Am J Dig Dis 13:209–212, 1968
Walker BE, Kelleher J, Davies T, Losowsky MS: Chemical faecal fat using single stools. Scand J Gastroenterol 6:277–280, 1971
Fourman LPR, Higgins G, Quelch P, O'Brien JPR, Witts LJ: The investigation and diagnosis of steatorrhea. Clin Sci 7:121–134, 1948
Annegers JH, Boutwell JW, Ivy AC: The effect of dietary fat on fecal fat excretion and subjective symptoms in man. Gastroenterology 10:486–495, 1948
Bär U: Stuhluntersuchung bei exokriner Pankreasinsuffizienz.In Pankreas. MM Forell (ed). Berlin, Springer, 1976, pp 435–439
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Schneider, M.U., Demling, L., Jones, S.A. et al. NMR spectrometry. Digest Dis Sci 32, 494–499 (1987). https://doi.org/10.1007/BF01296032
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01296032