Abstract
Helicobacter pylori seems, to be involved in the etiology of peptic ulcer and chronic gastritis. Histamine is fundamental in gastric secretion modulation, and some features ofH. pylori-associated gastritis (edema, vasodilatation, inflammatory cell infiltration) are typical of the histamine-mediated response Thisin vitro study has been undertaken as a preliminary step, in order to find a possible link betweenH Pylori and histamine release.H. pylori isolated from gastric biopsies, has been tested as whole washed bacterium, whole formalin-killed bacterium, and crude cell wall preparation with serosal mast cells obtained by density gradient centrifugation or elutriation from peritoneal, and pleural washings of male Wistar albino rats. Histamine release was assayed fluorimetrically. No significant histamine release was obtained by testing the various bacterial preparations alone with mast cells. On the contrary, whole washed cells, whole formalin-killed cells and crude cell walls fromH. pylori have been found to potentiate, compound 48/80 or calcium ionophore A23187-induced histamine release. Crude cell walls showed the highest activity, whereas filtered supernatants from broth cultures constantly appeared inactive. The enhancement in histamine release, differed between the different strains. The presentin vitro study, which shows the potentiating effect ofH. pylori on histamine release, may prove interesting; however, at present, clinical implications cannot be inferred and further studies as well asin vivo demonstrations are needed.
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This work was supported in part by grants from the Ministero dell'Università e della Ricerca Scientifica e Tecnologica and from the Regione Toscana.
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Bechi, P., Dei, R., Di Bello, M.G. et al. Helicobacter pylori potentiates histamine release from serosal rat mast cellsin vitro . Digest Dis Sci 38, 944–949 (1993). https://doi.org/10.1007/BF01295925
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DOI: https://doi.org/10.1007/BF01295925